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Nucleic Acids Res. 2005 January 1; 33(Database Issue): D294–D296.
Published online 2004 December 17. doi: 10.1093/nar/gki038.
PMCID: PMC539992
The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis
J. R. Cole,1* B. Chai,1 R. J. Farris,1 Q. Wang,1 S. A. Kulam,1 D. M. McGarrell,1 G. M. Garrity,1,2 and J. M. Tiedje1,2
1Center for Microbial Ecology and 2Department of Microbiology and Molecular Genetics, Biomedical Physical Sciences, Michigan State University, East Lansing, MI 48824-4320, USA
*To whom correspondence should be addressed. Tel: +1 517 432 4998; Fax: +1 517 353 8957; Email: rdpstaff/at/msu.edu
aThe online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions/at/oupjournals.org.
a© 2005, the authors
Received September 14, 2004; Revised September 24, 2004; Accepted September 24, 2004.
Abstract
The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along with analysis services and a phylogenetically consistent taxonomic framework for these data. Updated monthly, these services are made available through the RDP-II website (http://rdp.cme.msu.edu/). RDP-II release 9.21 (August 2004) contains 101 632 bacterial small subunit rRNA gene sequences in aligned and annotated format. High-throughput tools for initial taxonomic placement, identification of related sequences, probe and primer testing, data navigation and subalignment download are provided. The RDP-II email address for questions or comments is rdpstaff/at/msu.edu.