Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex

doi:10.1101/gad.1256804

Journal List > Genes Dev > v.18(23); Dec 1, 2004

Genes Dev. 2004 December 1; 18(23): 2893–2904.

doi: 10.1101/gad.1256804.

PMCID: PMC534650

Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex

James Brugarolas,¹ Kui Lei,² Rebecca L. Hurley,³ Brendan D. Manning,⁴ Jan H. Reiling,⁵ Ernst Hafen,⁵ Lee A. Witters,³ Leif W. Ellisen,² and William G. Kaelin, Jr.^1,^6,⁷

¹Dana-Farber Cancer Institute and Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA; ²Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, Massachusetts 02116, USA; ³Departments of Medicine and Biochemistry, Dartmouth Medical School and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA; ⁴Department of Genetics & Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA; ⁵Zoologisches Institut, Universitaet Zuerich, Winterthurerstr. 190, CH-8057 Zuerich, Switzerland; ⁶Howard Hughes Medical Institute, Chevy Chase, Maryland 20815, USA

⁷Corresponding author.E-MAIL william_kaelin/at/dfci.harvard.edu; FAX (617) 632-4760.

Received August 31, 2004; Accepted October 6, 2004.

This article has been cited by other articles in PMC.

Abstract

Mammalian target of rapamycin (mTOR) is a central regulator of protein synthesis whose activity is modulated by a variety of signals. Energy depletion and hypoxia result in mTOR inhibition. While energy depletion inhibits mTOR through a process involving the activation of AMP-activated protein kinase (AMPK) by LKB1 and subsequent phosphorylation of TSC2, the mechanism of mTOR inhibition by hypoxia is not known. Here we show that mTOR inhibition by hypoxia requires the TSC1/TSC2 tumor suppressor complex and the hypoxia-inducible gene REDD1/RTP801. Disruption of the TSC1/TSC2 complex through loss of TSC1 or TSC2 blocks the effects of hypoxia on mTOR, as measured by changes in the mTOR targets S6K and 4E-BP1, and results in abnormal accumulation of Hypoxia-inducible factor (HIF). In contrast to energy depletion, mTOR inhibition by hypoxia does not require AMPK or LKB1. Down-regulation of mTOR activity by hypoxia requires de novo mRNA synthesis and correlates with increased expression of the hypoxia-inducible REDD1 gene. Disruption of REDD1 abrogates the hypoxia-induced inhibition of mTOR, and REDD1 overexpression is sufficient to down-regulate S6K phosphorylation in a TSC1/TSC2-dependent manner. Inhibition of mTOR function by hypoxia is likely to be important for tumor suppression as TSC2-deficient cells maintain abnormally high levels of cell proliferation under hypoxia.

Keywords: Tuberous Sclerosis Complex, TSC1, TSC2, REDD1/RTP801, mTOR, Hypoxia

Tuberous sclerosis complex, a disease characterized by benign tumors in multiple tissues, results from mutations in either Tuberous Sclerosis Complex 1 (Tsc1) or 2 (Tsc2) (Cheadle et al. 2000). Tsc1 (also called hamartin) and Tsc2 (also called tuberin) form a protein complex (van Slegtenhorst et al. 1998) that integrates signals from a variety of sources, including growth factors (Gao and Pan 2001; Potter et al. 2001; Tapon et al. 2001) and energy stores (Inoki et al. 2003b), with the protein translation apparatus. Tsc2 functions as a GTPase-activating protein (GAP) toward the small G protein Rheb, which through a poorly understood mechanism controls mammalian target of rapamycin (mTOR), a central regulator of protein translation (Castro et al. 2003; Garami et al. 2003; Inoki et al. 2003a; Saucedo et al. 2003; Stocker et al. 2003; Tee et al. 2003; Y. Zhang et al. 2003).

Regulation of mTOR by growth factors has been intensively studied. In response to growth factor stimulation, phosphatidylinositol 3-kinase (PI3K) is activated, leading to the generation of phosphatidylinositol-3,4,5-triphosphate and the recruitment of Akt to the plasma membrane where it is activated by phosphorylation (Cantley 2002). Akt is a serine/threonine kinase that phosphorylates many effectors including Tsc2 (Dan et al. 2002; Inoki et al. 2002; Manning et al. 2002; Potter et al. 2002). The mechanism whereby Akt phosphorylation regulates Tsc2 function is controversial, but it is thought ultimately to lead to its inactivation, thereby allowing the accumulation of Rheb-GTP and the activation of mTOR. In support of the idea that Tsc1/Tsc2 plays a critical role in mTOR regulation by growth factors, cells deficient for Tsc1 or Tsc2 fail to down-regulate mTOR function in response to growth factor deprivation (Gao et al. 2002; Inoki et al. 2002; Jaeschke et al. 2002; Kwiatkowski et al. 2002; H. Zhang et al. 2003).

Recent studies have also shed light on how Tsc1/Tsc2 regulates mTOR function in response to changes in energy availability. AMP-activated protein kinase (AMPK) is a master regulator of energy metabolism that is activated in response to energy deprivation (Carling 2004). AMPK functions as a serine/threonine kinase and directly phosphorylates Tsc2 (Inoki et al. 2003b). Cells deficient for Tsc1/Tsc2, or producing a TSC2 variant that can not be phosphorylated by AMPK, fail to down-regulate mTOR in situations of energy deprivation (Inoki et al. 2003b). Signaling by energy depletion also involves the Lkb1 tumor suppressor protein, which is inactivated in Peutz-Jeghers syndrome. Lkb1 is a serine/threonine kinase that phosphorylates a variety of substrates, including AMPK (Hawley et al. 2003; Woods et al. 2003; Shaw et al. 2004b). AMPK activation with consequent Tsc1/Tsc2-mediated inhibition of mTOR by energy depletion requires Lkb1 (Corradetti et al. 2004; Shaw et al. 2004b). Thus under conditions that are adverse for growth, such as in the presence of reduced energy stores, mTOR function is inhibited, thereby down-regulating protein synthesis and conserving energy.

mTOR function is also regulated by amino acid availability (Gingras et al. 2001). Whereas mTOR regulation by growth factors and energy stores requires an intact Tsc1/Tsc2 complex, amino acids seem to regulate mTOR function through both Tsc1/Tsc2-dependent and independent pathways (Gao et al. 2002; H. Zhang et al. 2003).

mTOR is a conserved serine/threonine kinase that phosphorylates a series of substrates involved in protein translation including 4E-BP1 and S6K (Gingras et al. 2001). 4E-BP1 binds the translation initiation factor eIF-4E, preventing its interaction with other members of the eIF-4 complex and thereby inhibiting translation initiation of 5′ cap (7-methyl GTP) mRNAs. 4E-BP1 phosphorylation by mTOR, as well as other kinases in the PI3K pathway, relieves this inhibition, thereby promoting mRNA translation (Gingras et al. 2001).

In mammals, there are two S6K genes, S6K1 and S6K2, and each has two different splice forms. While S6K2 and the long form (~85 kDa) of S6K1 localize to the nucleus, the short form of S6K1 (~70 kDa) is cytoplasmic. S6K is thought to exist in an inactive, closed conformation, resulting from an intramolecular interaction between the catalytic domain and a pseudosubstrate domain (Fingar and Blenis 2004). There are at least eight phosphorylation sites in S6K1 and its activation requires a complex process of phosphorylation involving mTOR as well as other PI3K effectors. Phosphorylation in the linker region, at Ser 371 and Thr 389, is essential for S6K1 activation (Dufner and Thomas 1999). Thr 389 is the major rapamycin-sensitive site and can be phosphorylated by mTOR in vitro, suggesting that it is a direct mTOR target (Dufner and Thomas 1999). Phosphorylation at this site has also been shown to be regulated in response to changes in Tsc1/Tsc2 levels (Inoki et al. 2002; Jaeschke et al. 2002; Kwiatkowski et al. 2002; Manning et al. 2002; H. Zhang et al. 2003).

The best-characterized substrate of S6K1 is the ribosomal protein S6. S6 is an integral component of the 40S subunit that is required for cell proliferation but whose precise function is unclear (Volarevic et al. 2000). S6 is thought to be regulated primarily through successive phosphorylation events beginning at S236 and S235. Phosphorylation at these sites is rapamycin-sensitive and largely mediated by S6K (Dufner and Thomas 1999). Indeed, cells deficient for both S6K1 and S6K2 have profoundly reduced levels of S6 S235/236 phosphorylation (Pende et al. 2004). Thus, S235/236 phosphorylation provides an accurate readout for endogenous S6K activity.

The Hypoxia-inducible factor (Hif) has also been shown to be regulated by mTOR (Hudson et al. 2002). Hif is a heterodimeric transcription factor composed of a stable β and a labile α subunit whose levels are controlled by oxygen tension (Semenza 2000). In the presence of oxygen, Hif-α subunits are hydroxylated at specific prolyl residues and targeted for degradation by an E3 ubiquitin ligase that contains the von Hippel-Lindau tumor suppressor protein (pVHL) (Kaelin 2002). pVHL inactivation in patients with von Hippel-Lindau disease results in Hif up-regulation and the development of tumors. As in pVHL-deficient cells, Tsc2-deficient cells harbor increased levels of Hif-α relative to wild-type cells, especially under growth factor poor conditions (Brugarolas et al. 2003). Hif up-regulation in Tsc2-deficient cells is likely to result from increased mTOR activity as mTOR increases Hif stability and increased Hif levels in Tsc2-deficient cells can be normalized by treatment with rapamycin (Hudson et al. 2002; Brugarolas et al. 2003).

It was recently shown that mTOR function is regulated by hypoxia (Arsham et al. 2003). Hypoxia down-regulates 4E-BP1 phosphorylation and increases 4E-BP1 binding to eIF-4E at 5′ cap structures. Similarly, hypoxia down-regulates S6K phosphorylation at multiple sites including T389 and inhibits S6 phosphorylation. Hypoxia-induced inhibition of mTOR is dominant over mTOR activating signals from growth factors and nutrients and occurs independently of Hif-1α (Arsham et al. 2003). However, how mTOR function is regulated by hypoxia is not known.

Here we show that mTOR inhibition by hypoxia requires an intact Tsc1/Tsc2 complex. Furthermore, down-regulation of mTOR function by hypoxia requires de novo transcription and the expression of the hypoxiainducible Redd1/RTP801 gene.

Results

Tsc1/Tsc2 complex is required for mTOR regulation by hypoxia

To examine the contribution of the Tsc1/Tsc2 complex to the regulation of mTOR function by hypoxia, the effects of hypoxia on MEFs derived from Tsc2-deficient embryos were analyzed. Loss of Tsc2 results in lethality at approximately day 10.5 of gestation when it is difficult to obtain a large number of fibroblasts (Rennebeck et al. 1998). To extend the life span of the few fibroblasts that can be obtained from these embryos, mice were intercrossed with mice carrying mutations in p53, which enhances the life span of the limited number of MEFs that can be obtained (H. Zhang et al. 2003). In the experiments that follow, Tsc2^-/-;p53^-/- MEFs were compared with Tsc2^+/+;p53^-/- MEFs. For simplicity, these MEFs are referred to as “Tsc2^-/-” and “Tsc2^+/+,” respectively.

Tsc1/Tsc2 complex regulates mTOR in response to growth factors. As shown before, serum deprivation inhibits mTOR, as evidenced by decreased phosphorylation of the mTOR effector S6K (T389) (Fig. 1A

). S6K T389 is the major rapamycin-sensitive site and is required for S6K activity (Dufner and Thomas 1999). The down-regulation of S6K phosphorylation is associated with a decrease in S6 phosphorylation (S235/236). We also confirmed that mTOR inhibition by serum deprivation requires an intact Tsc1/Tsc2 complex and is abrogated in Tsc2^-/- MEFs (Fig. 1A

) (Jaeschke et al. 2002; H. Zhang et al. 2003). Rapamycin inhibits S6K phosphorylation and activity regardless of Tsc2 status, supporting the concept that Tsc1/Tsc2 functions upstream of mTOR (Fig. 1A

Figure 1.

Tsc2 regulates mTOR in response to hypoxia. (A) Western blot analysis of Tsc2^+/+ and Tsc2^-/- MEFs. (Hif) Hif-1α and/or Hif-2α; (S6K-P) S6K phosphorylated T389; (S6-P) S6 phosphorylated on S235/236. Left panel shows MEF in 0.05% serum or (more ...)

Tsc2^+/+ MEFs down-regulated S6K phosphorylation and activity in response to hypoxia (Fig. 1A

), in keeping with an earlier study using transformed human embryonic kidney (HEK293) cells (Arsham et al. 2003). Down-regulation of S6 phosphorylation in response to hypoxia was also demonstrable in mice exposed to 6% oxygen, suggesting that this phenomenon is not restricted to tissue culture (Supplementary Fig. 1). In contrast, hypoxia failed to down-regulate S6K and S6 phosphorylation in Tsc2^-/- MEFs (Fig. 1A

) and Tsc1^-/- mouse 3T3 cells (Fig. 1B

), indicating that mTOR inhibition by hypoxia requires an intact Tsc1/Tsc2 complex. Likewise hypoxia promoted the binding of 4E-BP1 to 7-methyl GTP (7mGTP) in Tsc2^+/+ cells but not Tsc2^-/- cells, consistent with Tsc2-dependent inhibition of 4E-BP1 phosphorylation by mTOR in response to hypoxia (Fig. 1C

). Taken together, these data indicate that mTOR inhibition by hypoxia requires a functional Tsc1/Tsc2 complex.

We previously showed that Hif-α (hereafter referred to as Hif) levels are regulated in response to growth factors through a mechanism that involves the Tsc1/Tsc2 complex and mTOR (Brugarolas et al. 2003; see also Fig. 1A

). As Tsc2^-/- MEFs failed to down-regulate mTOR activity in response to hypoxia, we postulated that Hif levels might also be affected. Tsc2^-/- MEFs were not impaired in the up-regulation of Hif in response to hypoxia. However, whereas Tsc2^+/+ MEFs down-regulated Hif with prolonged hypoxia, Hif levels remained elevated in Tsc2^-/- MEFs (Fig. 1D

). Consistent with the idea that increased Hif levels in Tsc2^-/- cells under prolonged hypoxia result from increased mTOR activity, treatment with rapamycin restored the down-regulation of Hif normally observed after prolonged hypoxia (Fig. 1E

The importance of Tsc2 in the regulation of mTOR by hypoxia is further supported by reconstitution experiments. Tsc2^-/- MEFs were infected with a retrovirus encoding epitope-tagged human Tsc2. Retrovirally transduced Tsc2^-/- MEFs achieved Tsc2 protein levels that were similar to endogenous levels in Tsc2^+/+ MEFs (Fig. 2A

). Reintroduction of Tsc2 into Tsc2^-/- MEFs restored the down-regulation of S6 phosphorylation by hypoxia (Fig. 2A

Figure 2.

Tsc2 is both necessary and sufficient for the regulation of S6 phosphorylation by hypoxia. (A) Western blot analysis of Tsc2^-/- MEFs retrovirally transduced with either a Tsc2 expression vector or an empty vector and treated with hypoxia for the indicated (more ...)

To determine the generalizability of our observations, the role of Tsc2 in mTOR regulation by hypoxia was examined in other cell types. Tsc2 knockdown with two different Tsc2 siRNAs in HEK293 and HeLa cervical carcinoma cells blocked the down-regulation of S6 phosphorylation by hypoxia (Fig. 2B

; Supplementary Fig. 2), indicating that Tsc2 is required for mTOR regulation by hypoxia in multiple cell types.

Tsc2 inactivation confers a proliferative advantage under hypoxia

Taken together, these data establish the importance of Tsc1/Tsc2 in the regulation of mTOR by hypoxia. As Tsc1/Tsc2 functions as a tumor suppressor, we asked whether disruption of this complex would affect cell proliferation under hypoxic conditions. In keeping with previous observations, the rates of cell proliferation of Tsc2^-/- and Tsc2^+/+ MEFs were very similar under normoxic conditions for several days (Fig. 3A

; H. Zhang et al. 2003). After 4 d in culture the rate of proliferation of Tsc2^+/+ cells began to decline relative to Tsc2^-/- cells, possibly due to depletion of nutrients and growth factors from the media. Consistent with this idea, Tsc2^-/- but not Tsc2^+/+ MEFs proliferate under conditions of serum deprivation (H. Zhang et al. 2003). We next measured the proliferation of Tsc2^-/- and Tsc2^+/+ cells under hypoxic conditions. To avoid confounding effects from media depletion, the media was changed daily. Under hypoxic conditions Tsc2^-/- MEFs exhibited a marked proliferative advantage compared with Tsc2^+/+ MEFs (Fig. 3B

). Increased proliferation of Tsc2^-/- MEFs under hypoxic conditions correlated with persistently elevated levels of S6 phosphorylation (Fig. 3C

) and was abrogated by treatment with rapamycin (Fig. 3B

). These data suggest that failure to down-regulate mTOR in response to hypoxia in Tsc2^-/- MEFs confers a growth advantage that might contribute to tumor formation in TSC patients. It should be noted that the growth inhibitory effect of hypoxia on Tsc2^+/+ MEFs in these assays is p53 independent since both the Tsc2^+/+ and Tsc2^-/- MEFs used here lack p53.

Figure 3.

Tsc2 loss confers a proliferative advantage under hypoxic conditions. (A) Proliferation rates of Tsc2^-/- and Tsc2^+/+ MEFs under normoxic conditions. (B) Proliferation rates under hypoxic conditions of Tsc2^+/+ and Tsc2^-/- MEFs (treated or not with rapamycin). (more ...)

Tsc1/Tsc2 regulation by hypoxia is AMPK and Lkb1 independent

Energy depletion results in Tsc1/Tsc2-mediated mTOR inhibition through a mechanism that involves Lkb1 and AMPK (Inoki et al. 2003b; Corradetti et al. 2004; Shaw et al. 2004a). Since hypoxia might also affect cellular energy stores, we sought to determine whether hypoxia regulated mTOR through a similar pathway. AICAR (5-aminoomidazole-4-carboxyamide), a cell-permeable AMPK agonist, inhibited mTOR and down-regulated S6 phosphorylation (Fig. 4A

; Kimura et al. 2003). As previously reported (Shaw et al. 2004b), Tsc2^-/- cells failed to down-regulate S6 phosphorylation in response to AICAR despite robust activation of AMPK as determined by AMPK phosphorylation in the T loop and the phosphorylation of its substrate acetyl-CoA carboxylase (ACC) (Fig. 4A

), thereby justifying the use of AICAR as an AMPK perturbant in the experiments described below.

Figure 4.

mTOR regulation by hypoxia is both AMPK and Lkb1 independent. (A) Western blot analysis of extracts of Tsc2^+/+ and Tsc2^-/- MEFs treated with AICAR for the indicated periods of time. (ACC-P) Acetyl-CoA carboxylase phosphorylated at S79; (AMPK-P) AMPK phosphorylated (more ...)

To ask whether hypoxia signals are transduced through an energy depletion pathway in our system, we first examined the effects of hypoxia on AMPK activity. After 4 h of hypoxia, S6 phosphorylation was markedly down-regulated without a detectable increase in AMPK activity, as measured by ACC phosphorylation (Fig. 4B

). The ability to detect ACC phosphorylation in response to AMPK activation was confirmed by studying cells treated with AICAR in parallel (Fig. 4B

). Furthermore, in vitro kinase assays with AMPK immunoprecipitates from the same lysates used for Figure 4B

indicated that hypoxia, in contrast to AICAR, did not increase and may have decreased AMPK kinase activity (Fig. 4C

). These results are consistent with a recent study of HEK293 cells exposed to hypoxia for 30 min (Arsham et al. 2003).

We next asked whether AMPK activity is necessary for the hypoxia-induced down-regulation of mTOR function. Pharmacological inhibition of AMPK with compound C (Inoki et al. 2003b) at doses sufficient to block the down-regulation of S6 phosphorylation by AICAR did not block the down-regulation of S6 phosphorylation by hypoxia (Fig. 4D

). Thus AMPK is required for AICAR signaling but not for hypoxia signaling.

Next we examined the requirements for the AMPK kinase Lkb1. Consistent with previous studies, Lkb1^-/- MEFs failed to down-regulate S6 phosphorylation in response to AICAR (Fig. 4E

; Shaw et al. 2004b). In contrast, we found that Lkb1 loss did not affect the down-regulation of S6 phosphorylation by hypoxia (Fig. 4F

). In addition, HeLa cells, which are defective for Lkb1 (Tiainen et al. 1999), also down-regulated S6 phosphorylation in response to hypoxia but not to AICAR (Supplementary Fig. 3) indicating that Lkb1, like AMPK, is dispensable for hypoxia signaling. We noted, however, that ACC and AMPK do become phosphorylated in HeLa cells after prolonged hypoxia, possibly due to a non-Lkb1 AMPK kinase (Supplementary Fig. 3). Nonetheless, inhibition of S6 phosphorylation occurred prior to appreciable AMPK activation in these cells, again in keeping with the idea that the acute down-regulation of mTOR in response to hypoxia is AMPK independent. It is possible that signaling to Tsc1/Tsc2 in response to chronic hypoxia involves both Lkb1/AMPK-dependent and independent pathways since prolonged hypoxia would predictably lead to ATP depletion and accumulation of AMP.

Regulation of mTOR by hypoxia requires Redd1 induction

To begin to unravel the signaling pathway whereby hypoxia regulates Tsc1/Tsc2, we asked whether this mechanism required de novo transcription. Provocatively, inhibition of transcription with actinomycin D blocked the down-regulation of S6 phosphorylation by hypoxia, indicating that de novo transcription is necessary for hypoxia signaling (Fig. 5A

). As a control actinomycin D alone did not affect baseline S6 phosphorylation (Fig. 5A

Figure 5.

Down-regulation of mTOR function by hypoxia requires Redd1. (A) Western blot analysis of Tsc2^+/+ MEFs pretreated (30 min prior to initiation of the hypoxia time course) or not, with Actinomycin D and exposed to hypoxia for the indicated periods of time. (more ...)

Recently, the Drosophila ortholog of the hypoxia-inducible Redd1 gene was isolated in a screen for suppressors of insulin signaling (Reiling and Hafen 2004). Redd1 was originally identified by several groups as a gene that is induced at the mRNA level in response to stresses such as hypoxia or DNA damage (Ellisen et al. 2002; Shoshani et al. 2002). Redd1 encodes a protein with a predicted MW of 25 kDa that lacks any known functional domains (Ellisen et al. 2002; Shoshani et al. 2002). Redd1 belongs to a family of highly conserved proteins that includes Redd2, which might also be regulated by hypoxia (Cuaz-Perolin et al. 2004). Redd orthologs have been shown to inhibit insulin signaling in Drosophila and epistasis analysis suggests that they act upstream of Tsc1/Tsc2 (Reiling and Hafen 2004).

To ascertain whether Redd1 was involved in the regulation of the mTOR by hypoxia, we first examined Redd1 induction in response to hypoxia in MEFs. Consistent with previous data, Redd1 mRNA levels were induced by hypoxia (Fig. 5B

; Shoshani et al. 2002). Redd2 expression, however, was not detected in MEFs (data not shown).

To ask whether Redd1 is necessary for hypoxia-induced down-regulation of mTOR function, we obtained Redd1^-/- MEFs (K. Lei and L.W. Ellisen, in prep.). In contrast to Redd1^+/+ MEFs, Redd1^-/- MEFs failed to down-regulate phosphorylation of S6K and S6 in response to hypoxia (Fig. 5C

). These data indicate that Redd1, like Tsc1 and Tsc2, is necessary for the hypoxia-induced down-regulation of mTOR signaling. This requirement was specific because Redd1^-/- MEFs, in contrast to Tsc2^-/- MEFs, down-regulated S6 phosphorylation in response to serum deprivation (Fig. 6A,B

). These data indicate that Redd1 regulates mTOR signaling only in response to certain stimuli, such as hypoxia.

Figure 6.

Tsc2 but not Redd1 is required for growth factor signaling. Western blot analysis of Tsc2^+/+ and Tsc2^-/- MEFs (A) or Redd1^+/+ and Redd1^-/- MEFs (B) after serum deprivation for the indicated periods of time.

Redd1 is sufficient to down-regulate S6K1 phosphorylation

We next asked whether Redd1 is sufficient to down-regulate mTOR function. For this purpose, the effects of Redd1 expression on S6K phosphorylation were determined. HEK293 cells were transfected with HA-tagged human Redd1 (R1) or a mutant lacking its evolutionarily conserved central region (R1dC; amino acids 96-153 out of 232). To exclude signal from untransfected cells, and thereby increase signal/noise ratio, a plasmid encoding HA-S6K1 (S6K) was included in the transfection mix. HA-S6K1 was then recovered by anti-HA immunoprecipitation and immunoblotted with an antibody specific for phospho-S6K1. Wild-type Redd1, but not mutant Redd1, down-regulated S6K1 phosphorylation (Fig. 7A

). As a control, S6K1 phosphorylation was abrogated in cells treated with rapamycin (Fig. 7A

). These data indicate that Redd1 is sufficient to down-regulate S6K1 phosphorylation and that this function requires the Redd1 central domain.

Figure 7.

Down-regulation of S6K1 phosphorylation by Redd1 and Redd2. (A) Western blot analysis of anti-HA immunoprecipitates (top) or whole-cell extracts (bottom) from HEK293 cells transfected with HA-tagged expression vectors encoding Redd1 (R1), Redd1 mutant (more ...)

Simultaneous overexpression of two Redd orthologs in Drosophila, Scylla and Charybdis, results in additive effects with respect to cell size (Reiling and Hafen 2004). Since cell size in Drosophila is controlled by S6K, we asked whether Redd2 also down-regulated S6K1 phosphorylation. For this purpose, we repeated these experiments with HA-Redd2 (R2), alone or in combination with Redd1. Redd2, like Redd1, inhibited S6K1 phosphorylation and the effects of Redd1 and Redd2 together were possibly additive (Fig. 7A

). While the same epitope tag in Redd1 (or 2) and S6K1, combined with immunoprecipitation, could theoretically confound our results, qualitatively similar effects were observed when S6K1 phosphorylation was analyzed directly from cell lysates (data not shown).

Down-regulation of S6K phosphorylation by Redd1 requires Tsc2

To ask whether Redd1 functions upstream of Tsc2, similar assays were performed in HeLa cells in which Tsc2 function was impaired using siRNA. The effect of Redd1 and Redd2 on S6K1 phosphorylation in HeLa cells transfected with irrelevant siRNA (Sc; scrambled) mirrored those observed in HEK293 cells (Fig. 7, cf. B and A

). In stark contrast, the effects of Redd1 and Redd2, alone or in combination, on S6K1 phosphorylation were completely abrogated in cells treated with either of two different Tsc2 siRNAs (a and b) (Fig. 7B

To evaluate the effects of Redd1 on endogenous (rather than ectopic) S6K as well as S6 phosphorylation, and its dependency on Tsc1/Tsc2 for signaling, we generated a HA-Redd1-inducible cell line (Fig. 7C

). These cells were transfected with siRNA (as above; Sc, Tsc2 a and b) and subsequently placed in media with tetracycline to induce HA-Redd1. Redd1 induction resulted in a profound down-regulation of endogenous S6K and S6 phosphorylation unless Tsc2 was down-regulated by siRNA (Fig. 7C

). Therefore down-regulation of S6K phosphorylation by Redd1 and Redd2 requires Tsc2. In addition, S6K phosphorylation in Redd1^-/- cells was down-regulated by treatment with rapamycin (data not shown), in keeping with the idea that Redd1 acts upstream of mTOR. While we cannot formally exclude that Redd1 acts in a parallel pathway, our data suggest that Redd1 acts upstream of the Tsc1/Tsc2 complex to down-regulate mTOR function in response to hypoxia.

Discussion

The data presented here indicate that the down-regulation of mTOR function by hypoxia requires Tsc1/Tsc2. Under conditions of hypoxia, Tsc2-deficient cells fail to down-regulate S6K phosphorylation (and activity) and accumulate Hif abnormally. Disruption of the Tsc1/Tsc2 complex results in increased cell proliferation under hypoxic conditions. Hypoxia signaling requires neither AMPK nor Lkb1 but does require de novo transcription and the expression of Redd1. Redd1 is necessary and sufficient for the down-regulation of mTOR by hypoxia.

Our data reveal that mTOR regulation by hypoxia requires Tsc1/Tsc2 in multiple cell types. Tsc2 was essential for hypoxia-induced mTOR down-regulation in three different cell types, MEFs, HEK293 cells, and HeLa cells. The requirement of Tsc2 for the down-regulation of mTOR in multiple diverse cell types suggests that mTOR regulation by hypoxia universally requires Tsc2. The regulation of mTOR function by hypoxia is likely to be important physiologically, as it occurs not only in cells grown in tissue culture, but also in vivo (Supplementary Fig. 1), and is likely impaired in tumors from TSC patients.

Exposure of Tsc2-deficient cells to hypoxia, in contrast to glucose (energy) depletion (Inoki et al. 2003b), did not result in a marked increase in cell death (determined by trypan blue exclusion; data not shown). Instead, our studies clearly demonstrate that loss of Tsc2, which functions as a tumor suppressor, confers a growth advantage to hypoxic cells. This phenomenon might contribute to the development of tumors in patients with tuberous sclerosis complex and lymphangioleiomyomatosis (LAM), which is also linked to Tsc2 mutations (Carsillo et al. 2000). Moreover, it raises the possibility that physiologically relevant levels of hypoxia in normal tissues, in a Tsc2-dependent manner, affect cell proliferation and the establishment of a post-mitotic state.

Tsc2 is involved in the down-regulation of Hif, especially under low serum conditions (Brugarolas et al. 2003) and, as shown here, in response to prolonged hypoxia. We and others have previously shown that increased Hif levels in Tsc2-deficient cells are associated with increased expression of Hif regulated genes including VEGF-A, which encodes for a potent angiogenic peptide (Nguyen-Vu et al. 2001; Brugarolas et al. 2003; El-Hashemite et al. 2003; Liu et al. 2003). Thus, prolonged hypoxia in Tsc2-deficient cells will further increase VEGF-A. Overproduction of VEGF-A almost certainly explains the angiogenic nature of neoplasms (hamartomas) observed in tuberous sclerosis complex disease and may play a causal role in LAM, which is a disease characterized by respiratory failure. The disease affects young women and arises from increased proliferation of smooth muscle and lymphatics in the lung parenchyma. While VEGF-A functions primarily on the blood vessel endothelium (Scavelli et al. 2004), it might also promote pulmonary lymphangiogenesis, thereby contributing to the pathogenesis of LAM. Increased pulmonary lymphangiogenesis would theoretically exacerbate hypoxia and establish a vicious cycle characterized by increased Hif accumulation in Tsc2-deficient pulmonary cells, leading to increased VEGF-A and lymphangiogenesis.

In our assays, changes in the phosphorylation of S6 (S235/236) in response to hypoxia were more robust than changes in the phosphorylation of S6K (T389) or changes in the amount of 4E-BP1 bound to ⁷mGTP. While the importance of S6K in the phosphorylation of S6 is well documented (Pende et al. 2004), Tsc1/Tsc2 might conceivably also regulate S6 phosphorylation in an mTOR-independent/S6K-independent manner. There is precedence for Tsc1/Tsc2 having rapamycin-insensitive, and hence probably mTOR-independent, activities (Brugarolas et al. 2003; Karbowniczek et al. 2004). The analysis of S6 phosphorylation is, however, biologically relevant, as S6 but not S6K (1 and 2) is essential for cell proliferation (Volarevic et al. 2000; Pende et al. 2004).

Our data indicate that hypoxia signaling to Tsc1/Tsc2 requires de novo transcription and the expression of the Redd1 gene. In keeping with published observations, we found that Redd1 mRNA is induced by hypoxia. The Redd1 gene contains a hypoxia response element in the promoter, which can be recognized by Hif-1 complexes (Shoshani et al. 2002). On the other hand, Redd1 is also induced in response to hypoxia in Hif-1α^-/- ES cells, albeit to a lesser extent (Shoshani et al. 2002). In addition, Hif-1α is dispensable for the down-regulation of mTOR in response to hypoxia (Arsham et al. 2003). These data suggest that induction of Redd1 by hypoxia can also be mediated by other Hif-α family members (such as Hif-2α) or perhaps by a Hif-independent mechanism.

Redd1 expression is sufficient to down-regulate S6K1 phosphorylation. This effect is abrogated by deleting the conserved central region of the protein (amino acids 96-153) and requires Tsc2. While it cannot be excluded that Redd1 functions in a parallel pathway, the simplest model is that Redd1 functions upstream of, and activates, Tsc1/Tsc2, which in turn inhibits mTOR. Genetic experiments in Drosophila are consistent with this model (Reiling and Hafen 2004).

It will be important to determine how, mechanistically, Redd1 affects Tsc1/Tsc2 function. We have thus far been unable to detect complex formation between Tsc1/Tsc2 and Redd1 (J. Brugarolas, unpubl.). In theory, Redd1 might, at least indirectly, affect Tsc1/Tsc2 GAP activity. In pilot experiments, we confirmed that anti-Tsc2 immunoprecipitates from Tsc2^+/+ cells, but not from Tsc2^-/- cells, display Rheb GAP activity. However, no difference in GAP activity was found when Tsc2 immunoprecipitates from normoxic and hypoxic cells were compared (B. Manning and J. Brugarolas, unpubl.). These data suggest that either hypoxia does not alter the intrinsic GAP activity of Tsc2 or that this assay is not sensitive enough to pick up small, but physiologically relevant, changes in Tsc2 GAP activity.

While our data indicate that Tsc1/Tsc2 is necessary for the down-regulation of mTOR after prolonged hypoxia (several days; see Fig. 3C

), it remains to be established whether Redd1 is also required in this setting. Indeed the early peak in Redd1 expression in response to hypoxia suggests that Redd1 might not play a role in mTOR regulation by chronic hypoxia. It is also possible that AMPK and Lkb1, although dispensable for the acute down-regulation of mTOR in response to acute hypoxia, play important roles in maintaining mTOR inhibition with prolonged hypoxia. Indeed, AMPK appears to be activated in HeLa cells with prolonged hypoxia, although this activation seems to be Lkb1 independent (Supplementary Fig. 3).

Pathways that regulate Tsc1/Tsc2 function are disrupted in cancer (Brugarolas and Kaelin 2004). Consequently, Redd1 might also function as a tumor suppressor and be mutated in human cancers. As Redd1 is involved in hypoxic signaling, but not growth factor signaling, the discovery of Redd1 mutations in tumors would emphasize the importance of hypoxia in the physiological regulation of Tsc1/Tsc2 tumor suppressor function.

Redd1 is induced by DNA damage, heat shock, and the p53 family member p63, in addition to being induced by hypoxia. This suggests that Redd1 may play a role in the regulation of mTOR by other forms of cellular stress. DNA damage can down-regulate mTOR signaling (Tee et al. 2003) but it is not known whether this requires Tsc1/Tsc2 and Redd1. Since overexpression of Redd2 can also down-regulate mTOR, situations that induce Redd2 expression would also predictably lead to the down-regulation of the mTOR pathway. Both Redd1 and Redd2 are expressed during development (Ellisen et al. 2002). However, while inactivation of the Drosophila Redd orthologs does not affect survival during normal development (Reiling and Hafen 2004), it diminishes survival under conditions of hypoxia and starvation, suggesting that Redd1 and Redd2 might both regulate cell growth in response to a variety of stresses.

Materials and methods

Reagents

p53^-/-;Tsc2^-/- and p53^-/-;Tsc2^+/+ MEFs, as well as Tsc1^-/- and Tsc1^+/+ 3T3 mouse cells, were a gift from H. Onda and D.J. Kwiatkowski (Brigham and Women's Hospital, Harvard Medical School, Boston, MA); Lkb1^+/+ and Lkb1^-/- MEFs were a gift from N. Bardeesy and R. DePinho (Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA); pcDNA3/FLAGHA-Tsc2 was a gift from F. Vazquez (Johns Hopkins University, Baltimore, MD); and pRK7/HA-S6K1 (rat αII isoform) was a gift from J. Blenis (Harvard Medical School, Boston, MA). The Hif antibody, which recognizes both Hif-1α and Hif-2α (J. Brugarolas, unpubl.) was a gift from J. Pouyssegur (Institute of Signaling, Developmental Biology and Cancer Research, Nice, France) (Richard et al. 1999). The AMPK antibody was described previously (Hamilton et al. 2001). The remaining antibodies were purchased from the following vendors: Sigma: Tubulin-α (clone B-5-1-2); Santa Cruz: Tsc2 (C20), HA (Y11); Covance: HA (HA11); or Cell Signaling: phosphorylated S6 S235/236, phosphorylated ACC S79, phosphorylated AMPK T172, p70 S6K, 4E-BP1, and eIF-4E. 7-Methyl GTP-sepharose was from Amersham. Rapamycin (Calbiochem, cat. 553210) was used at 25 nM from a 25-μM stock solution in methanol. AICAR (Calbiochem, cat. 123040) was used at 1 mM from a 100-mM stock solution in water. AMPKi, compound C (Merck) was used at 5 μM from a 10-mM stock in DMSO. Actinomycin D (Sigma) was used at 5 μg/mL from a 0.5-mg/mL stock in water. Tetracycline (Sigma) was used at 10 μg/mL from a 10 mg/mL stock in water.

Cloning

Human Tsc2 cDNA (exon 31 deletion isoform) was excised from pcDNA3/FLAG-HA-Tsc2 with BamH1 and XbaI. The XbaI site was blunt ended with Klenow prior to insert excision. The insert was ligated into pBabe-puro/HA previously cut with BamH1 and SnaBI to make pBabe/HA-Tsc2, which was confirmed by restriction digestion and cDNA end-sequencing. Redd1 and Redd2 cDNAs were isolated by PCR from primary human and murine fibroblasts, respectively, cloned into the pcDNA3 expression vector, and sequence verified. Redd1dC carries a deletion of amino acids 96-153 and was made by overlap PCR.

Tissue culture

Passage 10-18 p53^-/-;Tsc2^+/+ and p53^-/-;Tsc2^-/-, as well as 3T3 immortalized Tsc1^+/+ and Tsc1^-/- MEFs, were cultured in DMEM with high glucose (Invitrogen), 10% heat-inactivated FBS (Hyclone), and 1% P/S (Gibco), and exposed to hypoxia (In vivo2; Biotrace) where indicated (1% oxygen, 10% carbon dioxide at 37°C). Growth curves were determined by counting cells in triplicate wells sequentially for 4 d. Cell death was determined by scoring trypan blue positivity after staining with 0.4% trypan blue (Sigma). Lbk1^-/- and Redd1^-/- MEFs (and respective littermate controls) were used at an early passage (<10). For hypoxia experiments, Lbk1^-/- and Redd1^-/- MEFs were cultured in MEMα (Invitrogen) or DMEM, with 2% FBS and 1% P/S. The Tet-inducible Redd1 line was created by transfecting the T-REx-U2OS founder line (Invitrogen) with pcDNA4/TO (Invitrogen) carrying a Redd1 cDNA followed by clonal selection. Cells were transfected with siRNA, split 48 h later, and treated with tetracycline for 24 h prior to harvest.

Retroviral transduction

Phoenix amphotropic cells were transfected with empty pBabe/HA, or pBabe/HA-Tsc2 using Lipofectamine Plus (Invitrogen) following manufacturer's recommendations. Supernatant was collected at several intervals 36 h after transfection and used to infect exponentially growing Tsc2^-/- MEFs (passage 10) in the presence of polybrene (4 μg/mL). Cells were selected in 2 μg/mL puromycin for 6 d and utilized for experiments for two additional passages.

SiRNA transfections

siRNA transfections were as previously described (Brugarolas et al. 2003). For experiments involving transfection of both siRNA and plasmids, HeLa cells were seeded at very low density and transfected with siRNA using Oligofectamine (Invitrogen) per manufacturer's recommendations except that the amount of siRNA was tripled. Approximately 48 h later, when ~80% confluent, the cells were transfected with plasmid DNA using Lipofectamine Plus (Invitrogen) per the manufacturer's recommendations. SiRNA sequences: Sc (5′-CAAUGAGUAACAAUCCAUUGA-3′); Tsc2 (a) (5′-CAAUGAGUCACAGUCCUUUGA-3′) (Inoki et al. 2002); Tsc2 (b) (5′-AAAGUUCACCUACUGCUGGCA-3′).

Western blot and immunoprecipitation assays

Western blot analysis was as previously described (Brugarolas et al. 2003). For immunoprecipitations, cells were lysed in IP buffer (50 mM Tris HCl at pH 7.5, 150 mM NaCl, and 0.5% Igepal CA-630 [Sigma] supplemented with Complete Mini [Roche] and phosphatase inhibitors [Calbiochem]) for 20 min at 4°C. The lysates were precleared with protein A sepharose (Amersham), normalized for protein content, and immunoprecipitated with 2-3 μg anti-HA (Y11)/mg of protein overnight at 4°C. Immunoprecipitates were recovered on Protein A sepharose, washed three times with IP buffer, eluted with hot SDS sample buffer, and analyzed by SDS PAGE.

⁷mGTP binding assays

For ⁷mGTP binding assays, MEFs were lysed in IP buffer. The lysates were precleared by centrifugation, normalized for protein content, and incubated with ⁷mGTP-sepharose for 2 h at 4°C. The sepharose was recovered by centrifugation, washed with IP buffer four times, and boiled in 1× SDS loading buffer. Eluted proteins were analyzed by SDS-PAGE on a 12% gel.

Kinase assay

AMPK kinase assays were as described with minor modifications (Hamilton et al. 2001). Briefly, exponentially growing Tsc2^+/+ MEFs were lysed in IP buffer. Lysates were normalized for protein content prior to immunoprecipitation with AMPK antibody (Hamilton et al. 2001) or rabbit serum IgG under antibody excess conditions. Immunoprecipitates were incubated with ATP γ ³²P (NEN) in the presence or absence of SAMS peptide for 25 min at 30°C. No AMP was added. Reactions were spotted onto p81 paper and thoroughly washed with 0.75% phosphoric acid and acetone. Radioactivity of the filters was determined using a scintillation counter (Beckman LS 6500).

Northern blot

Total RNA was prepared from primary MEFs using RNA STAT-60 (Tel-Test). Ten micrograms total RNA per lane was electro-phoresed through a 6% formaldehyde/1% agarose gel, transferred to Hybond-N nylon membrane (Amersham), and hybridized to ³²P-labeled murine Redd1, Redd2, and GAPDH cDNA probes.

Animal hypoxia experiments

C57BL/6 mice 2-4 mo old were placed in the hypoxia chamber with abundant food and drink. Oxygen concentration was slowly decreased from 21% to 6% over 1 h. CO₂ was 1% and temperature 25°C.

Acknowledgments

We thank J. Cifuni, R. Pietravalle, and A. Sofer for technical assistance; H. Onda and D.J. Kwiatkowski for p53^-/-;Tsc2^-/- and p53^-/-;Tsc2^+/+, as well as immortalized Tsc1^+/+ and Tsc1^-/- MEFs; J. Pouyssegur for Hif antibody; F. Vazquez for Tsc2 expression vector; J. Blenis for S6K vector; and G. Zhou (Merck) for compound C. This work was supported by the Claudia Adams Barr Foundation (J.B.), the Mittleman Foundation (J.B.), the DOD (J.B. and W.G.K.), and NIH DK35712 (L.A.W.). W.G.K. is an HHMI Investigator. Redd1 reagents described in this paper should be requested from L.W.E.

Notes

Supplemental material is available at http://www.genesdev.org.

Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1256804.

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