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Proc Natl Acad Sci U S A. Nov 1, 1991; 88(21): 9770–9774.
PMCID: PMC52802

Polydnavirus DNA is integrated in the DNA of its parasitoid wasp host.

Abstract

The polydnavirus Campoletis sonorensis virus (CsV) is present in the oviducts of all adult C. sonorensis female wasps and appears to be required for these wasps to parasitize hosts successfully. Physical mapping, Southern blot analysis, and nucleotide sequence analysis demonstrate that the viral DNA B-specific sequences in cloned wasp DNA are colinear with viral genomic segment DNA B from nucleocapsids and are covalently linked to nonviral wasp sequences. Integrated DNA B terminates in 59-nucleotide imperfect direct repeats, but a single repeat exists in the extrachromosomal superhelical viral DNA B. Sequences near each junction form imperfect inverted repeats with sequences near the ends of an internal viral 540-base-pair repeat element gene. CsV appears to be the first documented integrated, nonretroviral DNA virus of insects and probably is vertically transmitted as a provirus.

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Selected References

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  • Norton WN, Vinson SB. Correlating the initiation of virus replication with a specific pupal developmental phase of an ichneumonid parasitoid. Cell Tissue Res. 1983;231(2):387–398. [PubMed]
  • Fleming JA, Blissard GW, Summers MD, Vinson SB. Expression of Campoletis sonorensis Virus in the Parasitized Host, Heliothis virescens. J Virol. 1983 Oct;48(1):74–78. [PMC free article] [PubMed]
  • Blissard GW, Vinson SB, Summers MD. Identification, Mapping, and In Vitro Translation of Campoletis sonorensis Virus mRNAs from Parasitized Heliothis virescens Larvae. J Virol. 1986 Jan;57(1):318–327. [PMC free article] [PubMed]
  • Stoltz DB, Vinson SB. Viruses and parasitism in insects. Adv Virus Res. 1979;24:125–171. [PubMed]
  • Edson KM, Vinson SB, Stoltz DB, Summers MD. Virus in a parasitoid wasp: suppression of the cellular immune response in the parasitoid's host. Science. 1981 Feb 6;211(4482):582–583. [PubMed]
  • Krell PJ, Stoltz DB. Unusual Baculovirus of the Parasitoid Wasp Apanteles melanoscelus: Isolation and Preliminary Characterization. J Virol. 1979 Mar;29(3):1118–1130. [PMC free article] [PubMed]
  • Krell PJ, Summers MD, Vinson SB. Virus with a Multipartite Superhelical DNA Genome from the Ichneumonid Parasitoid Campoletis sonorensis. J Virol. 1982 Sep;43(3):859–870. [PMC free article] [PubMed]
  • Jones D, Sreekrishna S, Iwaya M, Yang JN, Eberely M. Comparison of viral ultrastructure and DNA banding patterns from the reproductive tracts of Eastern and Western hemisphere Chelonus sp. (Braconidae: Hymenoptera). J Invertebr Pathol. 1986 Jan;47(1):105–115. [PubMed]
  • Vinson SB. Microplitis croceipes: inhibitions of the Heliothis zea defense reaction to Cardiochiles nigriceps. Exp Parasitol. 1977 Feb;41(1):112–117. [PubMed]
  • Stoltz DB, Guzo D, Cook D. Studies on polydnavirus transmission. Virology. 1986 Nov;155(1):120–131. [PubMed]
  • Fleming JA, Summers MD. Campoletis sonorensis Endoparasitic Wasps Contain Forms of C. sonorensis Virus DNA Suggestive of Integrated and Extrachromosomal Polydnavirus DNAs. J Virol. 1986 Feb;57(2):552–562. [PMC free article] [PubMed]
  • Blissard GW, Smith OP, Summers MD. Two related viral genes are located on a single superhelical DNA segment of the multipartite Campoletis sonorensis virus genome. Virology. 1987 Sep;160(1):120–134. [PubMed]
  • Theilmann DA, Summers MD. Physical Analysis of the Campoletis sonorensis Virus Multipartite Genome and Identification of a Family of Tandemly Repeated Elements. J Virol. 1987 Aug;61(8):2589–2598. [PMC free article] [PubMed]
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Holmes DS, Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Stoltz DB. Evidence for chromosomal transmission of polydnavirus DNA. J Gen Virol. 1990 May;71(Pt 5):1051–1056. [PubMed]
  • Leung PC, Teplow DB, Harshey RM. Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Nature. 1989 Apr 20;338(6217):656–658. [PubMed]

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