• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Nov 1, 1991; 88(21): 9690–9694.
PMCID: PMC52784

Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning.

Abstract

The complete primary structure of Fel dI (International Union of Immunological Societies nomenclature), the major allergen produced by the domestic cat, Felis domesticus, was determined by protein sequence analysis and cDNA cloning. Protein sequencing of Fel dI from an immunoaffinity-purified extract of house dust revealed that the allergen is composed of two polypeptide chains. Degenerate oligonucleotides derived from the protein sequence were used in polymerase chain reaction amplification of cat salivary gland cDNA to demonstrate that the two chains are encoded by different genes. Chain 1 of Fel dI shares amino acid homology with rabbit uteroglobin, while chain 2 is a glycoprotein with N-linked oligosaccharides.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Freidhoff LR, Meyers DA, Marsh DG. A genetic-epidemiologic study of human immune responsiveness to allergens in an industrial population. II. The associations among skin sensitivity, total serum IgE, age, sex, and the reporting of allergies in a stratified random sample. J Allergy Clin Immunol. 1984 Apr;73(4):490–499. [PubMed]
  • Bryant DH, Burns MW. Skin-prick test reactions to inhalant allergens in asthmatic patients. Med J Aust. 1976 Jun 12;1(24):918–924. [PubMed]
  • Sarsfield JK, Boyle AG, Rowell EM, Moriarty SC. Pet sensitivities in asthmatic children. Arch Dis Child. 1976 Mar;51(3):186–189. [PMC free article] [PubMed]
  • Pollart SM, Chapman MD, Fiocco GP, Rose G, Platts-Mills TA. Epidemiology of acute asthma: IgE antibodies to common inhalant allergens as a risk factor for emergency room visits. J Allergy Clin Immunol. 1989 May;83(5):875–882. [PubMed]
  • Sears MR, Herbison GP, Holdaway MD, Hewitt CJ, Flannery EM, Silva PA. The relative risks of sensitivity to grass pollen, house dust mite and cat dander in the development of childhood asthma. Clin Exp Allergy. 1989 Jul;19(4):419–424. [PubMed]
  • Ohman JL, Jr, Lowell FC. IgE antibody to cat allergens in an allergic population. J Allergy Clin Immunol. 1977 Nov;60(5):317–323. [PubMed]
  • Anderson MC, Baer H. Allergenically active components of cat allergen extracts. J Immunol. 1981 Sep;127(3):972–975. [PubMed]
  • Ohman JL, Jr, Lowell FC, Bloch KJ. Allergens of mammalian origin. III. Properties of a major feline allergen. J Immunol. 1974 Dec;113(6):1668–1677. [PubMed]
  • Løwenstein H, Lind P, Weeke B. Identification and clinical significance of allergenic molecules of cat origin. Part of the DAS 76 Study. Allergy. 1985 Aug;40(6):430–441. [PubMed]
  • Duffort O, Carreira J, Lombardero M. Characterization of the main IgE-binding components of cat dander. Int Arch Allergy Appl Immunol. 1987;84(4):339–344. [PubMed]
  • Leitermann K, Ohman JL., Jr Cat allergen 1: Biochemical, antigenic, and allergenic properties. J Allergy Clin Immunol. 1984 Aug;74(2):147–153. [PubMed]
  • de Groot H, van Swieten P, van Leeuwen J, Lind P, Aalberse RC. Monoclonal antibodies to the major feline allergen Fel d I. I. Serologic and biologic activity of affinity-purified Fel d I and of Fel d I-depleted extract. J Allergy Clin Immunol. 1988 Nov;82(5 Pt 1):778–786. [PubMed]
  • Van Metre TE, Jr, Marsh DG, Adkinson NF, Jr, Fish JE, Kagey-Sobotka A, Norman PS, Radden EB, Jr, Rosenberg GL. Dose of cat (Felis domesticus) allergen 1 (Fel d 1) that induces asthma. J Allergy Clin Immunol. 1986 Jul;78(1 Pt 1):62–75. [PubMed]
  • Luczynska CM, Li Y, Chapman MD, Platts-Mills TA. Airborne concentrations and particle size distribution of allergen derived from domestic cats (Felis domesticus). Measurements using cascade impactor, liquid impinger, and a two-site monoclonal antibody assay for Fel d I. Am Rev Respir Dis. 1990 Feb;141(2):361–367. [PubMed]
  • Ohman JL, Jr, Findlay SR, Leitermann KM. Immunotherapy in cat-induced asthma. Double-blind trial with evaluation of in vivo and in vitro responses. J Allergy Clin Immunol. 1984 Sep;74(3 Pt 1):230–239. [PubMed]
  • Sundin B, Lilja G, Graff-Lonnevig V, Hedlin G, Heilborn H, Norrlind K, Pegelow KO, Løwenstein H. Immunotherapy with partially purified and standardized animal dander extracts. I. Clinical results from a double-blind study on patients with animal dander asthma. J Allergy Clin Immunol. 1986 Mar;77(3):478–487. [PubMed]
  • Brown PR, Leitermann K, Ohman JL., Jr Distribution of cat allergen 1 in cat tissues and fluids. Int Arch Allergy Appl Immunol. 1984;74(1):67–70. [PubMed]
  • Anderson MC, Baer H, Ohman JL., Jr A comparative study of the allergens of cat urine, serum, saliva, and pelt. J Allergy Clin Immunol. 1985 Oct;76(4):563–569. [PubMed]
  • Dabrowski AJ, Van der Brempt X, Soler M, Seguret N, Lucciani P, Charpin D, Vervloet D. Cat skin as an important source of Fel d I allergen. J Allergy Clin Immunol. 1990 Oct;86(4 Pt 1):462–465. [PubMed]
  • Chapman MD, Aalberse RC, Brown MJ, Platts-Mills TA. Monoclonal antibodies to the major feline allergen Fel d I. II. Single step affinity purification of Fel d I, N-terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d I exposure. J Immunol. 1988 Feb 1;140(3):812–818. [PubMed]
  • Duffort OA, Carreira J, Nitti G, Polo F, Lombardero M. Studies on the biochemical structure of the major cat allergen Felis domesticus I. Mol Immunol. 1991 Apr-May;28(4-5):301–309. [PubMed]
  • O'Hehir RE, Garman RD, Greenstein JL, Lamb JR. The specificity and regulation of T-cell responsiveness to allergens. Annu Rev Immunol. 1991;9:67–95. [PubMed]
  • Berzofsky JA, Brett SJ, Streicher HZ, Takahashi H. Antigen processing for presentation to T lymphocytes: function, mechanisms, and implications for the T-cell repertoire. Immunol Rev. 1988 Dec;106:5–31. [PubMed]
  • Rothbard JB, Gefter ML. Interactions between immunogenic peptides and MHC proteins. Annu Rev Immunol. 1991;9:527–565. [PubMed]
  • Bjorkman PJ, Saper MA, Samraoui B, Bennett WS, Strominger JL, Wiley DC. Structure of the human class I histocompatibility antigen, HLA-A2. Nature. 1987 Oct 8;329(6139):506–512. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Morel AM, Delaage MA. Immunoanalysis of histamine through a novel chemical derivatization. J Allergy Clin Immunol. 1988 Oct;82(4):646–654. [PubMed]
  • Andrews PC, Dixon JE. A procedure for in situ alkylation of cystine residues on glass fiber prior to protein microsequence analysis. Anal Biochem. 1987 Mar;161(2):524–528. [PubMed]
  • Brauer AW, Oman CL, Margolies MN. Use of o-phthalaldehyde to reduce background during automated Edman degradation. Anal Biochem. 1984 Feb;137(1):134–142. [PubMed]
  • Simpson RJ, Nice EC. In situ cyanogen bromide cleavage of N-terminally blocked proteins in a gas-phase sequencer. Biochem Int. 1984 Jun;8(6):787–791. [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Gubler U, Hoffman BJ. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Gyllensten UB, Erlich HA. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652–7656. [PMC free article] [PubMed]
  • Collins JF, Coulson AF, Lyall A. The significance of protein sequence similarities. Comput Appl Biosci. 1988 Mar;4(1):67–71. [PubMed]
  • Lee CC, Wu XW, Gibbs RA, Cook RG, Muzny DM, Caskey CT. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science. 1988 Mar 11;239(4845):1288–1291. [PubMed]
  • Frohman MA, Dush MK, Martin GR. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998–9002. [PMC free article] [PubMed]
  • Roux KH, Dhanarajan P. A strategy for single site PCR amplification of dsDNA: priming digested cloned or genomic DNA from an anchor-modified restriction site and a short internal sequence. Biotechniques. 1990 Jan;8(1):48–57. [PubMed]
  • Rafnar T, Griffith IJ, Kuo MC, Bond JF, Rogers BL, Klapper DG. Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen. J Biol Chem. 1991 Jan 15;266(2):1229–1236. [PubMed]
  • Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. [PubMed]
  • Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. [PubMed]
  • Singh G, Katyal SL, Brown WE, Phillips S, Kennedy AL, Anthony J, Squeglia N. Amino-acid and cDNA nucleotide sequences of human Clara cell 10 kDa protein. Biochim Biophys Acta. 1988 Sep 7;950(3):329–337. [PubMed]
  • Mornon JP, Fridlansky F, Bally R, Milgrom E. X-ray crystallographic analysis of a progesterone-binding protein. The C2221 crystal form of oxidized uteroglobin at 2.2 A resolution. J Mol Biol. 1980 Mar 15;137(4):415–429. [PubMed]
  • Mukherjee DC, Agrawal AK, Manjunath R, Mukherjee AB. Suppression of epididymal sperm antigenicity in the rabbit by uteroglobin and transglutaminase in vitro. Science. 1983 Feb 25;219(4587):989–991. [PubMed]
  • Beato M, Baier R. Binding of progesterone to the proteins of the uterine luminal fluid. Identification of uteroglobin as the binding protein. Biochim Biophys Acta. 1975 Jun 12;392(2):346–356. [PubMed]
  • Miele L, Cordella-Miele E, Mukherjee AB. Uteroglobin: structure, molecular biology, and new perspectives on its function as a phospholipase A2 inhibitor. Endocr Rev. 1987 Nov;8(4):474–490. [PubMed]
  • Miele L, Cordella-Miele E, Facchiano A, Mukherjee AB. Novel anti-inflammatory peptides from the region of highest similarity between uteroglobin and lipocortin I. Nature. 1988 Oct 20;335(6192):726–730. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...