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Proc Natl Acad Sci U S A. Oct 1, 1991; 88(19): 8735–8739.

Induction of plasmacytomas secreting antigen-specific monoclonal antibodies with a retrovirus expressing v-abl and c-myc.


ABL-MYC, a recombinant murine retrovirus that expresses v-abl and c-myc, rapidly induces transplantable mono- or oligoclonal plasmacytomas in BALB/c mice. To determine if the targets for transformation of this retrovirus are antigen-committed B lymphocytes and to explore this system as an alternative technique for producing antigen-specific monoclonal antibodies, plasmacytomas were induced in mice that had been immunized with two different types of immunogens, hen egg white lysozyme and sheep red blood cells. The majority of these plasmacytomas secreted immunogen-specific antibodies. Plasmacytomas induced in unimmunized mice did not react with hen egg white lysozyme or sheep red blood cells. The specific antibodies were comparable in concentration, specificity, and affinity to monoclonal antibodies obtained with conventional hybridoma technology, but, in addition to IgGs and IgMs, they included specific IgA antibodies, which are rare among splenic-derived hybridomas. Our results demonstrate that a principal target for ABL-MYC is an antigen-committed B lymphocyte. In addition this procedure provides an alternative method for the production of monoclonal antibodies, without a requirement for hetero-caryon formation by cell fusion techniques.

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  • Potter M, Sklar MD, Rowe WP. Rapid viral induction of plasmacytomas in pristane-primed BALB-c mice. Science. 1973 Nov 9;182(4112):592–594. [PubMed]
  • Ohno S, Migita S, Wiener F, Babonits M, Klein G, Mushinski JF, Potter M. Chromosomal translocations activating myc sequences and transduction of v-abl are critical events in the rapid induction of plasmacytomas by pristane and abelson virus. J Exp Med. 1984 Jun 1;159(6):1762–1777. [PMC free article] [PubMed]
  • Clynes R, Wax J, Stanton LW, Smith-Gill S, Potter M, Marcu KB. Rapid induction of IgM-secreting murine plasmacytomas by pristane and an immunoglobulin heavy-chain promoter/enhancer-driven c-myc/v-Ha-ras retrovirus. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6067–6071. [PMC free article] [PubMed]
  • Troppmair J, Potter M, Wax JS, Rapp UR. An altered v-raf is required in addition to v-myc in J3V1 virus for acceleration of murine plasmacytomagenesis. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9941–9945. [PMC free article] [PubMed]
  • Kurie JM, Morse HC, 3rd, Principato MA, Wax JS, Troppmair J, Rapp UR, Potter M, Mushinski JF. v-myc and v-raf act synergistically to induce B-cell tumors in pristane-primed adult BALBC mice. Oncogene. 1990 Apr;5(4):577–582. [PubMed]
  • Rosenbaum H, Harris AW, Bath ML, McNeall J, Webb E, Adams JM, Cory S. An E mu-v-abl transgene elicits plasmacytomas in concert with an activated myc gene. EMBO J. 1990 Mar;9(3):897–905. [PMC free article] [PubMed]
  • Largaespada D, Kaehler D, Weissinger E, Mischak H, Mushinski F, Risser R. The activity of an ABL-MYC retrovirus in fibroblast cell lines and in lymphocytes. Curr Top Microbiol Immunol. 1990;166:91–96. [PubMed]
  • Weissinger EM, Largaespada D, Smith-Gill SJ, Risser R, Mushinski JF, Mischak H. A retrovirus expressing v-abl and c-myc induces plasmacytomas in 100% of adult pristane-primed BALB/c mice. Curr Top Microbiol Immunol. 1990;166:121–127. [PubMed]
  • Mann R, Mulligan RC, Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell. 1983 May;33(1):153–159. [PubMed]
  • Rowe WP, Pugh WE, Hartley JW. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. [PubMed]
  • Jolley ME, Wang CH, Ekenberg SJ, Zuelke MS, Kelso DM. Particle concentration fluorescence immunoassay (PCFIA): a new, rapid immunoassay technique with high sensitivity. J Immunol Methods. 1984 Feb 24;67(1):21–35. [PubMed]
  • Rousseau PG, Mallett CP, Smith-Gill SJ. A substantial proportion of the adult BALB/c available B cell repertoire consists of multireactive B cells. Mol Immunol. 1989 Oct;26(10):993–1006. [PubMed]
  • Friguet B, Chaffotte AF, Djavadi-Ohaniance L, Goldberg ME. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J Immunol Methods. 1985 Mar 18;77(2):305–319. [PubMed]
  • Stevens FJ. Modification of an ELISA-based procedure for affinity determination: correction necessary for use with bivalent antibody. Mol Immunol. 1987 Oct;24(10):1055–1060. [PubMed]
  • Mischak H, Kolch W, Hofer F, Weissinger E, Gessl A, Davidson WF, Aiello FB, Blaas D, Rapp UR. A raf/myc virus immortalized macrophage cell line which supports the growth of B-cell and B-cell hybridomas. Oncogene. 1990 Sep;5(9):1377–1382. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Mushinski JF, Bauer SR, Potter M, Reddy EP. Increased expression of myc-related oncogene mRNA characterizes most BALB/c plasmacytomas induced by pristane or Abelson murine leukemia virus. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1073–1077. [PMC free article] [PubMed]
  • Goff SP, Gilboa E, Witte ON, Baltimore D. Structure of the Abelson murine leukemia virus genome and the homologous cellular gene: studies with cloned viral DNA. Cell. 1980 Dec;22(3):777–785. [PubMed]
  • Stanton LW, Watt R, Marcu KB. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas. Nature. 1983 Jun 2;303(5916):401–406. [PubMed]
  • Max EE, Seidman JG, Leder P. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450–3454. [PMC free article] [PubMed]
  • Harper M, Lema F, Boulot G, Poljak RJ. Antigen specificity and cross-reactivity of monoclonal anti-lysozyme antibodies. Mol Immunol. 1987 Feb;24(2):97–108. [PubMed]
  • Miller DG, Adam MA, Miller AD. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol Cell Biol. 1990 Aug;10(8):4239–4242. [PMC free article] [PubMed]
  • Metzger DW, Ch'ng LK, Miller A, Sercarz EE. The expressed lysozyme-specific B cell repertoire. I. Heterogeneity in the monoclonal anti-hen egg white lysozyme specificity repertoire, and its difference from the in situ repertoire. Eur J Immunol. 1984 Jan;14(1):87–93. [PubMed]

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