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J Virol. Dec 1978; 28(3): 865–876.
PMCID: PMC525811

DNA of Bacillus subtilis bacteriophage SPP1: physical mapping and localization of the origin of replication.

Abstract

The genome of Bacillus subtilis bacteriophage SPP1, a linear, 28.5-megadalton DNA duplex, was mapped by analysis with the restriction endonucleases endo R.Sal I, Sma I, Xba I, Bgl I, Bgl II, and EcoRI. The SPP1 genome, like that of the Salmonella typhimurium phage, P22, was found to be a terminally repetitious, circularly permuted molecule. 6-(p-Hydroxyphenylazo)uracil, a selective, reversible inhibitor of SPP1 DNA synthesis, was exploited to synchronize the initiation of genome replication and to selectively label the site of its initiation with radioactive thymidine. Restriction endonuclease analysis of the distribution of the label located the origin of replicative synthesis at an area approximately 0.2 genome length from one molecular terminus.

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Selected References

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  • Arrand JR, Keller W, Roberts RJ. Extent of terminal repetition in adenovirus 2 DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):401–407. [PubMed]
  • Danna KJ, Sack GH, Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. [PubMed]
  • Englund PT. The 3'-terminal nucleotide sequences of T7 DNA. J Mol Biol. 1972 May 14;66(2):209–224. [PubMed]
  • FARMER JL, ROTHMAN F. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS. J Bacteriol. 1965 Jan;89:262–263. [PMC free article] [PubMed]
  • Flock JI. Deletion mutants of temperate Bacillus subtilis bacteriophage phi105. Mol Gen Genet. 1977 Oct 24;155(3):241–247. [PubMed]
  • Jackson EN, Jackson DA, Deans RJ. EcoRI analysis of bacteriophage P22 DNA packaging. J Mol Biol. 1978 Jan 25;118(3):365–388. [PubMed]
  • Jackson EN, Miller HI, Adams ML. EcoRI restriction endonuclease cleavage site map of bacteriophage P22DNA. J Mol Biol. 1978 Jan 25;118(3):347–363. [PubMed]
  • Klotz G. Direction of SPP1 DNA replication in transfected B. subtilis cells. Mol Gen Genet. 1973 Jan 18;120(1):95–100. [PubMed]
  • Mulder C, Delius H. Specificity of the break produced by restricting endonuclease R 1 in Simian virus 40 DNA, as revealed by partial denaturation mapping. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3215–3219. [PMC free article] [PubMed]
  • Mulder C, Sharp PA, Delius H, Pettersson U. Specific fragmentation of DNA of adenovirus serotypes 3, 5, 7, and 12, and adeno-simian virus 40 hybrid virus Ad2+ND1 by restriction endonuclease R.EcoRI. J Virol. 1974 Jul;14(1):68–77. [PMC free article] [PubMed]
  • Riva S, Polsinelli M, Falaschi A. A new phage of Bacillus subtilis with infectious DNA having separable strands. J Mol Biol. 1968 Jul 28;35(2):347–356. [PubMed]
  • Rowley SD, Brown NC. Bacillus subtilis DNA polymerase III is required for the replication of DNA of bacteriophages SPP-1 and phi 105. J Virol. 1977 Feb;21(2):493–496. [PMC free article] [PubMed]
  • Schleif R, Greenblatt J, Davis RW. Dual control of arabinose genes on transducing phage lambda-dara. J Mol Biol. 1971 Jul 14;59(1):127–150. [PubMed]
  • Smith HO, Nathans D. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973 Dec 15;81(3):419–423. [PubMed]
  • Spatz HC, Trautner TA. One way to do experiments on gene conversion? Transfection with heteroduplex SPP1 DNA. Mol Gen Genet. 1970;109(1):84–106. [PubMed]
  • Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. [PMC free article] [PubMed]
  • Tye BK, Chan RK, Botstein D. Packaging of an oversize transducing genome by Salmonella phage P22. J Mol Biol. 1974 Jan 5;85(4):485–500. [PubMed]
  • Tye BK, Huberman JA, Botstein D. Non-random circular permutation of phage P22 DNA. J Mol Biol. 1974 Jan 5;85(4):501–528. [PubMed]
  • Wensink PC, Finnegan DJ, Donelson JE, Hogness DS. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell. 1974 Dec;3(4):315–325. [PubMed]
  • Yamamoto KR, Alberts BM, Benzinger R, Lawhorne L, Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. [PubMed]
  • Yasbin RE, Young FE. Transduction in Bacillus subtilis by bacteriophage SPP1. J Virol. 1974 Dec;14(6):1343–1348. [PMC free article] [PubMed]

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