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Proc Natl Acad Sci U S A. Oct 1, 1991; 88(19): 8539–8543.
PMCID: PMC52544

Vitamin D3 binding protein (group-specific component) is a precursor for the macrophage-activating signal factor from lysophosphatidylcholine-treated lymphocytes.

Abstract

A brief (30 min) treatment of mouse peritoneal cells (mixture of nonadherent lymphocytes and adherent macrophages) with 1-20 micrograms of lysophosphatidylcholine (lyso-PC) per ml in serum-supplemented RPMI medium 1640, followed by a 3-hr cultivation of the adherent cells alone, results in a greatly enhanced Fc receptor-mediated phagocytic activity of macrophages. This rapid process of macrophage activation was found to require a serum factor, the vitamin D3 binding protein (the human protein is known as group-specific component; Gc). Efficient activation of macrophages was achieved by using medium containing purified human Gc protein. Analysis of intercellular signal transmission among nonadherent (B and T) cells revealed that lyso-PC-treated B cells modify Gc protein to yield a proactivating factor, which can be converted by T cells to the macrophage-activating factor. This rapid generation process of the macrophage-activating factor was also demonstrated by stepwise incubation of Gc protein with lyso-PC-treated B-cell ghosts and untreated T-cell ghosts, suggesting that Gc protein is modified by preexisting membranous enzymes to yield the macrophage-activating factor. Incubation of Gc protein with a mixture of beta-galactosidase and sialidase efficiently generated the macrophage-activating factor. Stepwise incubation of Gc protein with B- or T-cell ghosts and sialidase or beta-galactosidase revealed that Gc protein is modified by beta-galactosidase of B cells and sialidase of T cells to yield the macrophage-activating factor. Administration to mice of a minute amount (4-10 pg per mouse) of in vitro, enzymatically generated macrophage-activating factor resulted in a greatly enhanced (3- to 7-fold) ingestion activity of macrophages.

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  • Ngwenya BZ, Yamamoto N. Activation of peritoneal macrophages by lysophosphatidylcholine. Biochim Biophys Acta. 1985 Mar 29;839(1):9–15. [PubMed]
  • Yamamoto N, Ngwenya BZ. Activation of mouse peritoneal macrophages by lysophospholipids and ether derivatives of neutral lipids and phospholipids. Cancer Res. 1987 Apr 15;47(8):2008–2013. [PubMed]
  • Yamamoto N, Ngwenya BZ, Sery TW, Pieringer RA. Activation of macrophages by ether analogues of lysophospholipids. Cancer Immunol Immunother. 1987;25(3):185–192. [PubMed]
  • Snyder F, Wood R. Alkyl and alk-1-enyl ethers of glycerol in lipids from normal and neoplastic human tissues. Cancer Res. 1969 Jan;29(1):251–257. [PubMed]
  • Howard BV, Morris HP, Bailey JM. Ether-lipids, -glycerol phosphate dehydrogenase, and growth rate in tumors and cultured cells. Cancer Res. 1972 Jul;32(7):1533–1538. [PubMed]
  • Yamamoto N, St Claire DA, Jr, Homma S, Ngwenya BZ. Activation of mouse macrophages by alkylglycerols, inflammation products of cancerous tissues. Cancer Res. 1988 Nov 1;48(21):6044–6049. [PubMed]
  • Ngwenya BZ, Yamamoto N. Contribution of lysophosphatidylcholine-treated nonadherent cells to mechanism of macrophage activation. Proc Soc Exp Biol Med. 1990 Feb;193(2):118–124. [PubMed]
  • Homma S, Yamamoto N. Activation process of macrophages after in vitro treatment of mouse lymphocytes with dodecylglycerol. Clin Exp Immunol. 1990 Feb;79(2):307–313. [PMC free article] [PubMed]
  • Homma S, Millman I, Yamamoto N. A serum factor for macrophage activation after in vitro dodecylglycerol treatment of mouse lymphocytes. Immunol Cell Biol. 1990 Apr;68(Pt 2):137–142. [PubMed]
  • Yamamoto N, Homma S, Millman I. Identification of the serum factor required for in vitro activation of macrophages. Role of vitamin D3-binding protein (group specific component, Gc) in lysophospholipid activation of mouse peritoneal macrophages. J Immunol. 1991 Jul 1;147(1):273–280. [PubMed]
  • Kimura H, Saheki S, Yoshida K, Ohsawa M. Characterization of purified group-specific components (Gc 1 and Gc 2 proteins) from human plasma. Nihon Hoigaku Zasshi. 1985 Apr;39(2):113–123. [PubMed]
  • Bianco C, Griffin FM, Jr, Silverstein SC. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. [PMC free article] [PubMed]
  • Svasti J, Bowman BH. Human group-specific component. Changes in electrophoretic mobility resulting from vitamin D binding and from neuraminidase digestion. J Biol Chem. 1978 Jun 25;253(12):4188–4194. [PubMed]
  • Viau M, Constans J, Debray H, Montreuil J. Isolation and characterization of the O-glycan chain of the human vitamin-D binding protein. Biochem Biophys Res Commun. 1983 Nov 30;117(1):324–331. [PubMed]
  • AIDS and the Gc protein. Lancet. 1987 Jun 13;1(8546):1377–1378. [PubMed]
  • Potier M, Lu Shun Yan D, Womack JE. Neuraminidase deficiency in the mouse. FEBS Lett. 1979 Dec 15;108(2):345–348. [PubMed]
  • Landolfi NF, Leone J, Womack JE, Cook RG. Activation of T lymphocytes results in an increase in H-2-encoded neuraminidase. Immunogenetics. 1985;22(2):159–167. [PubMed]
  • Taira S, Nariuchi H. Possible role of neuraminidase in activated T cells in the recognition of allogeneic Ia. J Immunol. 1988 Jul 15;141(2):440–446. [PubMed]
  • Coppenhaver DH, Sollenne NP, Bowman BH. Post-translational heterogeneity of the human vitamin D-binding protein (group-specific component). Arch Biochem Biophys. 1983 Oct 1;226(1):218–223. [PubMed]

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