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Proc Natl Acad Sci U S A. 1991 Sep 15; 88(18): 8052–8056.

The initiator directs the assembly of a transcription factor IID-dependent transcription complex.


Highly purified RNA polymerase II was found to be able to weakly recognize the initiator (Inr) present in the adenovirus IVa2 and major late promoters. The association of RNA polymerase II with the Inr was enhanced by the general transcription factors. The Inr was capable of directing the formation of a DNA-protein complex. Transcription competent complexes on the adenovirus major late and IVa2 promoters appear to be formed by alternative pathways mediated through the Inr and/or "TATA" motif. The presence of both motifs, however, is required for efficient transcription utilizing a discrete start site. Complexes formed at either site required transcription factor TFIID, the TATA binding protein. Consistent with this observation, a TFIID requirement was demonstrated for transcription from a mutant adenovirus major late promoter construct lacking a functional TATA motif.

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  • McKnight S, Tjian R. Transcriptional selectivity of viral genes in mammalian cells. Cell. 1986 Sep 12;46(6):795–805. [PubMed]
  • Dynan WS, Tjian R. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. Nature. 316(6031):774–778. [PubMed]
  • Corden J, Wasylyk B, Buchwalder A, Sassone-Corsi P, Kedinger C, Chambon P. Promoter sequences of eukaryotic protein-coding genes. Science. 1980 Sep 19;209(4463):1406–1414. [PubMed]
  • Breathnach R, Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. [PubMed]
  • Smale ST, Baltimore D. The "initiator" as a transcription control element. Cell. 1989 Apr 7;57(1):103–113. [PubMed]
  • Mermelstein FH, Flores O, Reinberg D. Initiation of transcription by RNA polymerase II. Biochim Biophys Acta. 1989 Sep 21;1009(1):1–10. [PubMed]
  • Saltzman AG, Weinmann R. Promoter specificity and modulation of RNA polymerase II transcription. FASEB J. 1989 Apr;3(6):1723–1733. [PubMed]
  • Davison BL, Egly JM, Mulvihill ER, Chambon P. Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences. Nature. 1983 Feb 24;301(5902):680–686. [PubMed]
  • Nakajima N, Horikoshi M, Roeder RG. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID. Mol Cell Biol. 1988 Oct;8(10):4028–4040. [PMC free article] [PubMed]
  • Buratowski S, Hahn S, Guarente L, Sharp PA. Five intermediate complexes in transcription initiation by RNA polymerase II. Cell. 1989 Feb 24;56(4):549–561. [PubMed]
  • Maldonado E, Ha I, Cortes P, Weis L, Reinberg D. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. Mol Cell Biol. 1990 Dec;10(12):6335–6347. [PMC free article] [PubMed]
  • Reinberg D, Roeder RG. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE. J Biol Chem. 1987 Mar 5;262(7):3310–3321. [PubMed]
  • Reinberg D, Horikoshi M, Roeder RG. Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site. J Biol Chem. 1987 Mar 5;262(7):3322–3330. [PubMed]
  • Baker CC, Ziff EB. Promoters and heterogeneous 5' termini of the messenger RNAs of adenovirus serotype 2. J Mol Biol. 1981 Jun 25;149(2):189–221. [PubMed]
  • Carcamo J, Maldonado E, Cortes P, Ahn MH, Ha I, Kasai Y, Flint J, Reinberg D. A TATA-like sequence located downstream of the transcription initiation site is required for expression of an RNA polymerase II transcribed gene. Genes Dev. 1990 Sep;4(9):1611–1622. [PubMed]
  • Concino MF, Lee RF, Merryweather JP, Weinmann R. The adenovirus major late promoter TATA box and initiation site are both necessary for transcription in vitro. Nucleic Acids Res. 1984 Oct 11;12(19):7423–7433. [PMC free article] [PubMed]
  • Inostroza J, Flores O, Reinberg D. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE. J Biol Chem. 1991 May 15;266(14):9304–9308. [PubMed]
  • Flores O, Ha I, Reinberg D. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF. J Biol Chem. 1990 Apr 5;265(10):5629–5634. [PubMed]
  • Lee RF, Concino MF, Weinmann R. Genetic profile of the transcriptional signals from the adenovirus major late promoter. Virology. 1988 Jul;165(1):51–56. [PubMed]
  • Smale ST, Schmidt MC, Berk AJ, Baltimore D. Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4509–4513. [PMC free article] [PubMed]
  • Hu SL, Manley JL. DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2. Proc Natl Acad Sci U S A. 1981 Feb;78(2):820–824. [PMC free article] [PubMed]
  • Ruden DM, Ma J, Ptashne M. No strict alignment is required between a transcriptional activator binding site and the "TATA box" of a yeast gene. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4262–4266. [PMC free article] [PubMed]
  • Stucka R, Feldmann H. An element of symmetry in yeast TATA-box binding protein transcription factor IID. Consequence of an ancestral duplication? FEBS Lett. 1990 Feb 26;261(2):223–225. [PubMed]
  • Means AL, Farnham PJ. Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site. Mol Cell Biol. 1990 Feb;10(2):653–661. [PMC free article] [PubMed]
  • Farnham PJ, Means AL. Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter. Mol Cell Biol. 1990 Apr;10(4):1390–1398. [PMC free article] [PubMed]
  • Beaupain D, Eléouët JF, Roméo PH. Initiation of transcription of the erythroid promoter of the porphobilinogen deaminase gene is regulated by a cis-acting sequence around the cap site. Nucleic Acids Res. 1990 Nov 25;18(22):6509–6515. [PMC free article] [PubMed]
  • Hariharan N, Perry RP. Functional dissection of a mouse ribosomal protein promoter: significance of the polypyrimidine initiator and an element in the TATA-box region. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1526–1530. [PMC free article] [PubMed]

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