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J Virol. Aug 1977; 23(2): 234–239.
PMCID: PMC515825

5'-Terminal and internal methylated nucleosides in herpes simplex virus type 1 mRNA.


RNA labeled with [methyl-3H]methionine and/or [32P]orthophosphate was isolated from the polyribosomes of herpes simplex virus (HSV) types 1-infected cells and separated into polyadenylylated [poly(A+)]and non-polyadenylylated [poly(A-)] fractions. Virus-specific RNA was obtained by hybridization in liquid to either excess HSV DNA or filters containing immobilized HSV DNA. Analysis in denaturing sucrose gradients indicated that HSV-specific poly(A+) RNA sedimented in a broad peak, with a modal S value of 20. The ratio of [3H]methyl to 32P decreased with increasing size of RNA, suggesting that each RNA chain contains a similar sumber of methyl groups. Further analysis indicated an average of one RNase-resistant structure of the type m7G(5')pppNmpNp or m7G(5')pppNmpNmpNp per 2,780 nucleotides. The following components were identified in the 5'-terminal oligonucleotides of polyribosome-associated HSV-specific poly(A+) and poly(A-) RNA: 7-methylguanosine, N6,2'-O-dimethyladenosine, and the 2'-O-methyl derivatives of guanosine, adenosine, uridine, and denosine, and the 2'-O-methyl derivatives of guanosine, adenosine, uridine, and cytidine. The most common 5'-terminal sequences were m7G(5')pppm6Am and m7G(5')pppGm. An additional modified nucleoside, N6-methyladenosine, was present in an internal position of HSV-specific RNA.

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Selected References

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  • Bachenheimer SL, Roizman B. Ribonucleic acid synthesis in cells infected with herpes simplex virus. VI. Polyadenylic acid sequences in viral messenger ribonucleic acid. J Virol. 1972 Oct;10(4):875–879. [PMC free article] [PubMed]
  • Bartkoski M, Roizman B. RNA synthesis in cells infected with herpes simple virus. XIII. Differences in the methylation patterns of viral RNA during the reproductive cycle. J Virol. 1976 Dec;20(3):583–588. [PMC free article] [PubMed]
  • Desrosiers R, Friderici K, Rottman F. Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3971–3975. [PMC free article] [PubMed]
  • Dubin DT, Taylor RH. The methylation state of poly A-containing messenger RNA from cultured hamster cells. Nucleic Acids Res. 1975 Oct;2(10):1653–1668. [PMC free article] [PubMed]
  • EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. [PubMed]
  • Furuichi Y, Morgan M, Shatkin AJ, Jelinek W, Salditt-Georgieff M, Darnell JE. Methylated, blocked 5 termini in HeLa cell mRNA. Proc Natl Acad Sci U S A. 1975 May;72(5):1904–1908. [PMC free article] [PubMed]
  • Harada F, Kimura F, Nishimura S. Nucleotide sequence of oligonucleotides derived from Escherichia coli valine transfer RNA by ribonuclease T1 digestion: comparison of the sequences neighboring 3'- and 5'-terminals and anticodon region of Escherichia coli valine transfer RNA with those of yeast valine transfer RNA. Biochim Biophys Acta. 1969 Jun 17;182(2):590–592. [PubMed]
  • Hashimoto SI, Green M. Multiple methylated cap sequences in adenovirus type 2 early mRNA. J Virol. 1976 Nov;20(2):425–435. [PMC free article] [PubMed]
  • Lavi S, Shatkin AJ. Methylated simian virus 40-specific RNA from nuclei and cytoplasm of infected BSC-1 cells. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2012–2016. [PMC free article] [PubMed]
  • Lewis JB, Atkins JF, Anderson CW, Baum PR, Gesteland RF. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344–1348. [PMC free article] [PubMed]
  • Moss B, Gershowitz A, Weber LA, Baglioni C. Histone mRNAs contain blocked and methylated 5' terminal sequences but lack methylated nucleosides at internal positions. Cell. 1977 Jan;10(1):113–120. [PubMed]
  • Moss B, Koczot F. Sequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA. J Virol. 1976 Feb;17(2):385–392. [PMC free article] [PubMed]
  • Palmiter RD. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. [PubMed]
  • Perry RP, Kelley DE, Friderici K, Rottman F. The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus. Cell. 1975 Apr;4(4):387–394. [PubMed]
  • Perry RP, Scherrer K. The methylated constituents of globin mRNA. FEBS Lett. 1975 Sep 1;57(1):73–78. [PubMed]
  • Silvertien S, Millette R, Jones P, Roizman B. RNA synthesis in cells infected with herpes simplex virus. XII. Sequence complexity and properties of RNA differing in extent of adenylation. J Virol. 1976 Jun;18(3):977–991. [PMC free article] [PubMed]
  • Sommer S, Salditt-Georgieff M, Bachenheimer S, Darnell JE, Furuichi Y, Morgan M, Shatkin AJ. The methylation of adenovirus-specific nuclear and cytoplasmic RNA. Nucleic Acids Res. 1976 Mar;3(3):749–765. [PMC free article] [PubMed]
  • Stringer JR, Holland LE, Swanstrom RI, Pivo K, Wagner EK. Quantitation of herpes simplex virus type 1 RNA in infected HeLa cells. J Virol. 1977 Mar;21(3):889–901. [PMC free article] [PubMed]
  • Surrey S, Nemer M. Methylated blocked 5' terminal sequences of sea urchin embryo messenger RNA classes containing and lacking poly(A). Cell. 1976 Dec;9(4 Pt 1):589–595. [PubMed]
  • Swanstrom RI, Pivo K, Wagner EK. Restricted transcription of the herpes simplex virus genome occurring early after infection and in the presence of metabolic inhibitors. Virology. 1975 Jul;66(1):140–150. [PubMed]
  • Wagner EK, Roizman B. Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells. J Virol. 1969 Jul;4(1):36–46. [PMC free article] [PubMed]
  • Wagner EK, Swanstrom RI, Stafford MG. Transcription of the herpes simplex virus genome in human cells. J Virol. 1972 Oct;10(4):675–682. [PMC free article] [PubMed]
  • Wei CM, Gershowitz A, Moss B. Methylated nucleotides block 5' terminus of HeLa cell messenger RNA. Cell. 1975 Apr;4(4):379–386. [PubMed]
  • Wei CM, Gershowitz A, Moss B. 5'-Terminal and internal methylated nucleotide sequences in HeLa cell mRNA. Biochemistry. 1976 Jan 27;15(2):397–401. [PubMed]
  • Wei CM, Moss B. Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014–3018. [PMC free article] [PubMed]
  • Wei CM, Moss B. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA. Proc Natl Acad Sci U S A. 1975 Jan;72(1):318–322. [PMC free article] [PubMed]
  • Wei CM, Moss B. Nucleotide sequences at the N6-methyladenosine sites of HeLa cell messenger ribonucleic acid. Biochemistry. 1977 Apr 19;16(8):1672–1676. [PubMed]
  • Yaniv M, Barrell BG. Nucleotide sequence of E. coli B tRNA1-Val. Nature. 1969 Apr 19;222(5190):278–279. [PubMed]

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