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Proc Natl Acad Sci U S A. May 1, 1991; 88(9): 3671–3675.
PMCID: PMC51514

Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.

Abstract

By using a potential-dependent J-aggregate-forming delocalized lipophilic cation, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine++ + iodide (JC-1), we find that membrane potentials across mitochondria in a living cell can be heterogeneous. Remarkably, even within a long contiguous mitochondrion, regional heterogeneity in membrane potentials appears to be possible.

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Selected References

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  • Mitchell P. Keilin's respiratory chain concept and its chemiosmotic consequences. Science. 1979 Dec 7;206(4423):1148–1159. [PubMed]
  • Chen LB. Mitochondrial membrane potential in living cells. Annu Rev Cell Biol. 1988;4:155–181. [PubMed]
  • Bereiter-Hahn J. Behavior of mitochondria in the living cell. Int Rev Cytol. 1990;122:1–63. [PubMed]
  • Davis S, Weiss MJ, Wong JR, Lampidis TJ, Chen LB. Mitochondrial and plasma membrane potentials cause unusual accumulation and retention of rhodamine 123 by human breast adenocarcinoma-derived MCF-7 cells. J Biol Chem. 1985 Nov 5;260(25):13844–13850. [PubMed]
  • Chen LB. Fluorescent labeling of mitochondria. Methods Cell Biol. 1989;29:103–123. [PubMed]
  • Reers M, Kelly RA, Smith TW. Calcium and proton activities in rat cardiac mitochondria. Effect of matrix environment on behaviour of fluorescent probes. Biochem J. 1989 Jan 1;257(1):131–142. [PMC free article] [PubMed]
  • Ion transport in liver mitochondria. Energy barrier and stoicheometry of aerobic K+ translocation. Eur J Biochem. 1969 Jan;7(3):418–426. [PubMed]
  • Lee C, Chen LB. Dynamic behavior of endoplasmic reticulum in living cells. Cell. 1988 Jul 1;54(1):37–46. [PubMed]

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