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Proc Natl Acad Sci U S A. Mar 15, 1991; 88(6): 2093–2097.
PMCID: PMC51175

Dendritic location of neural BC1 RNA.

Abstract

In nerve cells, a specialized protein synthetic machinery is thought to operate in local compartments of dendrites, in particular beneath synaptic junctions, and thereby to facilitate swift adjustments of the postsynaptic protein repertoire in situ. This notion has been supported by the identification of polyribosomes and selected mRNAs in those compartments. In this study, we report the discovery of a specific RNA polymerase III transcript in dendrites. This RNA, a noncoding, 152-nucleotide-long, single-gene transcript known as BC1 RNA, is expressed almost exclusively in the nervous system. In adult rats as well as in immature rats in late developmental stages, BC1 RNA has been located in the dendrites and somata of a subset of neurons in the central and peripheral nervous system. The colocalization of BC1 RNA with dendritic mRNAs and polyribosomes may indicate a role--possibly within the functional unit of a high molecular mass ribonucleoprotein particle--in specific pre- or posttranslational processes in postsynaptic compartments of neurons.

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Selected References

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  • Davis L, Banker GA, Steward O. Selective dendritic transport of RNA in hippocampal neurons in culture. Nature. 1987 Dec 3;330(6147):477–479. [PubMed]
  • Steward O, Levy WB. Preferential localization of polyribosomes under the base of dendritic spines in granule cells of the dentate gyrus. J Neurosci. 1982 Mar;2(3):284–291. [PubMed]
  • Steward O. Alterations in polyribosomes associated with dendritic spines during the reinnervation of the dentate gyrus of the adult rat. J Neurosci. 1983 Jan;3(1):177–188. [PubMed]
  • Steward O, Falk PM. Protein-synthetic machinery at postsynaptic sites during synaptogenesis: a quantitative study of the association between polyribosomes and developing synapses. J Neurosci. 1986 Feb;6(2):412–423. [PubMed]
  • Steward O, Reeves TM. Protein-synthetic machinery beneath postsynaptic sites on CNS neurons: association between polyribosomes and other organelles at the synaptic site. J Neurosci. 1988 Jan;8(1):176–184. [PubMed]
  • Garner CC, Tucker RP, Matus A. Selective localization of messenger RNA for cytoskeletal protein MAP2 in dendrites. Nature. 1988 Dec 15;336(6200):674–677. [PubMed]
  • Kleiman R, Banker G, Steward O. Differential subcellular localization of particular mRNAs in hippocampal neurons in culture. Neuron. 1990 Dec;5(6):821–830. [PubMed]
  • Bruckenstein DA, Lein PJ, Higgins D, Fremeau RT., Jr Distinct spatial localization of specific mRNAs in cultured sympathetic neurons. Neuron. 1990 Dec;5(6):809–819. [PubMed]
  • Burgin KE, Waxham MN, Rickling S, Westgate SA, Mobley WC, Kelly PT. In situ hybridization histochemistry of Ca2+/calmodulin-dependent protein kinase in developing rat brain. J Neurosci. 1990 Jun;10(6):1788–1798. [PubMed]
  • Gordon-Weeks PR. RNA transport in dendrites. Trends Neurosci. 1988 Aug;11(8):342–343. [PubMed]
  • Black MM, Baas PW. The basis of polarity in neurons. Trends Neurosci. 1989 Jun;12(6):211–214. [PubMed]
  • Cleveland DW. Microtubule MAPping. Cell. 1990 Mar 9;60(5):701–702. [PubMed]
  • Kennedy MB. Regulation of synaptic transmission in the central nervous system: long-term potentiation. Cell. 1989 Dec 1;59(5):777–787. [PubMed]
  • Jones OT, Kunze DL, Angelides KJ. Localization and mobility of omega-conotoxin-sensitive Ca2+ channels in hippocampal CA1 neurons. Science. 1989 Jun 9;244(4909):1189–1193. [PubMed]
  • Walter P, Blobel G. Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum. Nature. 1982 Oct 21;299(5885):691–698. [PubMed]
  • Sutcliffe JG, Milner RJ, Gottesfeld JM, Reynolds W. Control of neuronal gene expression. Science. 1984 Sep 21;225(4668):1308–1315. [PubMed]
  • DeChiara TM, Brosius J. Neural BC1 RNA: cDNA clones reveal nonrepetitive sequence content. Proc Natl Acad Sci U S A. 1987 May;84(9):2624–2628. [PMC free article] [PubMed]
  • Owens GP, Chaudhari N, Hahn WE. Brain "identifier sequence" is not restricted to brain: similar abundance in nuclear RNA of other organs. Science. 1985 Sep 20;229(4719):1263–1265. [PubMed]
  • Neve RL, Finch EA, Bird ED, Benowitz LI. Growth-associated protein GAP-43 is expressed selectively in associative regions of the adult human brain. Proc Natl Acad Sci U S A. 1988 May;85(10):3638–3642. [PMC free article] [PubMed]
  • Murase K, Ryu PD, Randic M. Excitatory and inhibitory amino acids and peptide-induced responses in acutely isolated rat spinal dorsal horn neurons. Neurosci Lett. 1989 Aug 14;103(1):56–63. [PubMed]
  • Tiedge H. The use of UV light as a cross-linking agent for cells and tissue sections in in situ hybridization. DNA Cell Biol. 1991 Mar;10(2):143–147. [PubMed]
  • Alho H, Fremeau RT, Jr, Tiedge H, Wilcox J, Bovolin P, Brosius J, Roberts JL, Costa E. Diazepam binding inhibitor gene expression: location in brain and peripheral tissues of rat. Proc Natl Acad Sci U S A. 1988 Sep;85(18):7018–7022. [PMC free article] [PubMed]
  • De Camilli P, Miller PE, Navone F, Theurkauf WE, Vallee RB. Distribution of microtubule-associated protein 2 in the nervous system of the rat studied by immunofluorescence. Neuroscience. 1984 Apr;11(4):817–846. [PubMed]
  • Dowling JE, Boycott BB. Organization of the primate retina: electron microscopy. Proc R Soc Lond B Biol Sci. 1966 Nov 15;166(2):80–111. [PubMed]
  • Monaghan DT, Holets VR, Toy DW, Cotman CW. Anatomical distributions of four pharmacologically distinct 3H-L-glutamate binding sites. Nature. 1983 Nov 10;306(5939):176–179. [PubMed]
  • Skene JH. Axonal growth-associated proteins. Annu Rev Neurosci. 1989;12:127–156. [PubMed]
  • Goslin K, Schreyer DJ, Skene JH, Banker G. Development of neuronal polarity: GAP-43 distinguishes axonal from dendritic growth cones. Nature. 1988 Dec 15;336(6200):672–674. [PubMed]
  • Oestreicher AB, Gispen WH. Comparison of the immunocytochemical distribution of the phosphoprotein B-50 in the cerebellum and hippocampus of immature and adult rat brain. Brain Res. 1986 Jun 11;375(2):267–279. [PubMed]
  • Benowitz LI, Apostolides PJ, Perrone-Bizzozero N, Finklestein SP, Zwiers H. Anatomical distribution of the growth-associated protein GAP-43/B-50 in the adult rat brain. J Neurosci. 1988 Jan;8(1):339–352. [PubMed]
  • McKinnon RD, Shinnick TM, Sutcliffe JG. The neuronal identifier element is a cis-acting positive regulator of gene expression. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3751–3755. [PMC free article] [PubMed]

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