• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. Sep 15, 1998; 102(6): 1125–1131.
PMCID: PMC509095

Coordinated clearance of periciliary liquid and mucus from airway surfaces.

Abstract

Airway surface liquid is comprised of mucus and an underlying, watery periciliary liquid (PCL). In contrast to the well-described axial transport of mucus along airway surfaces via ciliary action, theoretical analyses predict that the PCL is nearly stationary. Conventional and confocal microscopy of fluorescent microspheres and photoactivated fluorescent dyes were used with well-differentiated human tracheobronchial epithelial cell cultures exhibiting spontaneous, radial mucociliary transport to study the movements of mucus and PCL. These studies showed that the entire PCL is transported at approximately the same rate as mucus, 39.2+/-4.7 and 39.8+/-4.2 micrometer/sec, respectively. Removing the mucus layer reduced PCL transport by > 80%, to 4.8+/-0.6 micrometer/sec, a value close to that predicted from theoretical analyses of the ciliary beat cycle. Hence, the rapid movement of PCL is dependent upon the transport of mucus. Mucus-dependent PCL transport was spatially uniform and exceeded the rate expected for pure frictional coupling with the overlying mucus layer; hence, ciliary mixing most likely accelerates the diffusion of momentum from mucus into the PCL. The cephalad movement of PCL along airway epithelial surfaces makes this mucus-driven transport an important component of salt and water physiology in the lung in health and disease.

Full Text

The Full Text of this article is available as a PDF (2.7M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Yoneda K. Mucous blanket of rat bronchus: an ultrastructural study. Am Rev Respir Dis. 1976 Nov;114(5):837–842. [PubMed]
  • Sanderson MJ, Sleigh MA. Ciliary activity of cultured rabbit tracheal epithelium: beat pattern and metachrony. J Cell Sci. 1981 Feb;47:331–347. [PubMed]
  • Boucher RC. Human airway ion transport. Part one. Am J Respir Crit Care Med. 1994 Jul;150(1):271–281. [PubMed]
  • Boucher RC. Human airway ion transport. Part two. Am J Respir Crit Care Med. 1994 Aug;150(2):581–593. [PubMed]
  • Quinton PM. Viscosity versus composition in airway pathology. Am J Respir Crit Care Med. 1994 Jan;149(1):6–7. [PubMed]
  • Smith JJ, Travis SM, Greenberg EP, Welsh MJ. Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface fluid. Cell. 1996 Apr 19;85(2):229–236. [PubMed]
  • Kilburn KH. A hypothesis for pulmonary clearance and its implications. Am Rev Respir Dis. 1968 Sep;98(3):449–463. [PubMed]
  • Satir P, Sleigh MA. The physiology of cilia and mucociliary interactions. Annu Rev Physiol. 1990;52:137–155. [PubMed]
  • Blake JR, Sleigh MA. Mechanics of ciliary locomotion. Biol Rev Camb Philos Soc. 1974 Feb;49(1):85–125. [PubMed]
  • Fulford GR, Blake JR. Muco-ciliary transport in the lung. J Theor Biol. 1986 Aug 21;121(4):381–402. [PubMed]
  • King M, Agarwal M, Shukla JB. A planar model for mucociliary transport: effect of mucus viscoelasticity. Biorheology. 1993 Jan-Feb;30(1):49–61. [PubMed]
  • Gray TE, Guzman K, Davis CW, Abdullah LH, Nettesheim P. Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am J Respir Cell Mol Biol. 1996 Jan;14(1):104–112. [PubMed]
  • Wu R, Yankaskas J, Cheng E, Knowles MR, Boucher R. Growth and differentiation of human nasal epithelial cells in culture. Serum-free, hormone-supplemented medium and proteoglycan synthesis. Am Rev Respir Dis. 1985 Aug;132(2):311–320. [PubMed]
  • Kaartinen L, Nettesheim P, Adler KB, Randell SH. Rat tracheal epithelial cell differentiation in vitro. In Vitro Cell Dev Biol Anim. 1993 Jun;29A(6):481–492. [PubMed]
  • Shah PL, Scott SF, Knight RA, Marriott C, Ranasinha C, Hodson ME. In vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosis. Thorax. 1996 Feb;51(2):119–125. [PMC free article] [PubMed]
  • Vasconcellos CA, Allen PG, Wohl ME, Drazen JM, Janmey PA, Stossel TP. Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin. Science. 1994 Feb 18;263(5149):969–971. [PubMed]
  • Yguerabide J, Schmidt JA, Yguerabide EE. Lateral mobility in membranes as detected by fluorescence recovery after photobleaching. Biophys J. 1982 Oct;40(1):69–75. [PMC free article] [PubMed]
  • Rahmoune H, Shephard KL. State of airway surface liquid on guinea pig trachea. J Appl Physiol (1985) 1995 Jun;78(6):2020–2024. [PubMed]
  • Eliezer N, Sadé J, Silberberg A, Nevo AC. The role of mucus in transport by cilia. Am Rev Respir Dis. 1970 Jul;102(1):48–52. [PubMed]
  • Wills PJ, Garcia Suarez MJ, Rutman A, Wilson R, Cole PJ. The ciliary transportability of sputum is slow on the mucus-depleted bovine trachea. Am J Respir Crit Care Med. 1995 Apr;151(4):1255–1258. [PubMed]
  • Gatto LA. Cholinergic and adrenergic stimulation of mucociliary transport in the rat trachea. Respir Physiol. 1993 May;92(2):209–217. [PubMed]
  • Mercer RR, Russell ML, Roggli VL, Crapo JD. Cell number and distribution in human and rat airways. Am J Respir Cell Mol Biol. 1994 Jun;10(6):613–624. [PubMed]
  • Serafini SM, Michaelson ED. Length and distribution of cilia in human and canine airways. Bull Eur Physiopathol Respir. 1977 Jul-Aug;13(4):551–559. [PubMed]
  • Yeates DB, Aspin N, Levison H, Jones MT, Bryan AC. Mucociliary tracheal transport rates in man. J Appl Physiol. 1975 Sep;39(3):487–495. [PubMed]
  • Jiang C, Finkbeiner WE, Widdicombe JH, McCray PB, Jr, Miller SS. Altered fluid transport across airway epithelium in cystic fibrosis. Science. 1993 Oct 15;262(5132):424–427. [PubMed]
  • Folkesson HG, Matthay MA, Hasegawa H, Kheradmand F, Verkman AS. Transcellular water transport in lung alveolar epithelium through mercury-sensitive water channels. Proc Natl Acad Sci U S A. 1994 May 24;91(11):4970–4974. [PMC free article] [PubMed]
  • TOREMALM NG. The daily amoung of tracheo-bronchial secretions in man. A method for continuous tracheal aspiration in laryngectomized and tracheotomized patients. Acta Otolaryngol Suppl. 1960;158:43–53. [PubMed]
  • Folkesson HG, Matthay MA, Frigeri A, Verkman AS. Transepithelial water permeability in microperfused distal airways. Evidence for channel-mediated water transport. J Clin Invest. 1996 Feb 1;97(3):664–671. [PMC free article] [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...