• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jclinpathJournal of Clinical PathologyCurrent TOCInstructions for authors
J Clin Pathol. Apr 1995; 48(4): 364–367.
PMCID: PMC502557

Tissue and serum pepsinogen I and II in gastric cancer identified using immunohistochemistry and rapid ELISA.


AIMS--To investigate the immunohistochemical expression and the serum concentrations of pepsinogen I and II in different histological types of gastric cancer as compared with other gastric disorders. METHODS--Formalin fixed, paraffin wax embedded tissue specimens of 38 gastric cancers obtained from surgical cases were used for the immunohistochemical studies performed with the avidin-biotin complex method using monoclonal antibodies against purified pepsinogen I and II. Pepsinogen concentrations from serum obtained from the above patients, from patients with various other gastric disorders, and from normal controls were measured with a rapid non-radioactive one step enzyme linked immunosorbent assay (ELISA). RESULTS--Eight of 38 (21%) and seven of 38 (18%) gastric carcinomas showed immunoreactivity to pepsinogen I and pepsinogen II, respectively, without any correlation to histological classification or differentiation. Decreased pepsinogen I concentrations and low pepsinogen I:II ratios were found specifically in cases of gastric carcinoma and polyp, in good accordance with the immunohistochemical results. CONCLUSIONS--Low serum pepsinogen I concentrations and a low pepsinogen I:II ratio are predictive of gastric neoplasia, correlating with low tissue immunoreactivity to monoclonal antibodies raised against pepsinogen I and II. For mass screening of gastric disease including carcinoma, ELISA using a one step immunoassay performed in the present study is a rapid and reliable non-radioactive method of detecting serum pepsinogen. In addition, immunohistochemical studies showed that pepsinogen production may be increased or diminished as a result of tumour histogenesis, depending on the area of origin and the processes of cell transformation and dedifferentiation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Samloff IM. Pepsinogens, pepsins, and pepsin inhibitors. Gastroenterology. 1971 Apr;60(4):586–604. [PubMed]
  • Samloff IM, Secrist DM, Passaro E., Jr A study of the relationship between serum group I pepsinogen levels and gastric acid secretion. Gastroenterology. 1975 Dec;69(6):1196–1200. [PubMed]
  • Huang SC, Miki K, Furihata C, Ichinose M, Shimizu A, Oka H. Enzyme-linked immunosorbent assays for serum pepsinogens I and II using monoclonal antibodies--with data on peptic ulcer and gastric cancer. Clin Chim Acta. 1988 Jun 30;175(1):37–50. [PubMed]
  • Miki K, Ichinose M, Kawamura N, Matsushima M, Ahmad HB, Kimura M, Sano J, Tashiro T, Kakei N, Oka H, et al. The significance of low serum pepsinogen levels to detect stomach cancer associated with extensive chronic gastritis in Japanese subjects. Jpn J Cancer Res. 1989 Feb;80(2):111–114. [PubMed]
  • Huang SC, Miki K, Sano J, Ichinose M, Kawamura N, Oka H, Hirano K, Furihata C, Masugi Y, Takahashi K. Pepsinogens I and II in gastric cancer: an immunohistochemical study using monoclonal antibodies. Jpn J Cancer Res. 1988 Oct;79(10):1139–1146. [PubMed]
  • Samloff IM. Pepsinogens I and II: purification from gastric mucosa and radioimmunoassay in serum. Gastroenterology. 1982 Jan;82(1):26–33. [PubMed]
  • Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. [PubMed]
  • Konishi N, Kitahori Y, Shimoyama T, Lin JC, Hiasa Y. Monoclonal antibody against rat renal cell tumor-associated antigen as a new tool for the analysis of renal tumorigenesis. Jpn J Cancer Res. 1989 Aug;80(8):771–777. [PubMed]
  • Samloff IM, Varis K, Ihamaki T, Siurala M, Rotter JI. Relationships among serum pepsinogen I, serum pepsinogen II, and gastric mucosal histology. A study in relatives of patients with pernicious anemia. Gastroenterology. 1982 Jul;83(1 Pt 2):204–209. [PubMed]
  • Samloff IM, Varis K, Ihamaki T, Siurala M, Rotter JI. Relationships among serum pepsinogen I, serum pepsinogen II, and gastric mucosal histology. A study in relatives of patients with pernicious anemia. Gastroenterology. 1982 Jul;83(1 Pt 2):204–209. [PubMed]
  • Biasco G, Paganelli GM, Vaira D, Holton J, Di Febo G, Brillanti S, Miglioli M, Barbara L, Samloff IM. Serum pepsinogen I and II concentrations and IgG antibody to Helicobacter pylori in dyspeptic patients. J Clin Pathol. 1993 Sep;46(9):826–828. [PMC free article] [PubMed]
  • Busby-Earle RM, Williams AR, Piris J. Pepsinogens in gastric carcinomas. Hum Pathol. 1986 Oct;17(10):1031–1035. [PubMed]
  • Stemmermann GN, Samloff IM, Hayashi T. Pepsinogens I and II in carcinoma of the stomach: an immunohistochemical study. Appl Pathol. 1985;3(3):159–163. [PubMed]
  • Samloff IM, Liebman WM. Cellular localization of the group II pepsinogens in human stomach and duodenum by immunofluorescence. Gastroenterology. 1973 Jul;65(1):36–42. [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Group


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...