• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jclinpathJournal of Clinical PathologyCurrent TOCInstructions for authors
J Clin Pathol. Mar 1994; 47(3): 209–213.
PMCID: PMC501896

Assessment of proliferative activity in leukaemic bone marrow using the monoclonal antibody Ki-67.

Abstract

AIMS--To investigate proliferative activity in leukaemic and lymphomatous bone marrow infiltrates and to assess the feasibility of transport of specimens among institutions. METHODS--Proliferative activity in bone marrow trephine cryosections from 99 patients with non-Hodgkin's lymphoma (NHL), 23 patients with acute myeloid leukaemia (AML), 11 with acute lymphoblastic leukaemia (ALL), and two with acute undifferentiated leukaemia (AUL) was investigated. Infiltration was seen in 52 out of 99 cases of NHL on bone marrow cryosections. A score was devised to assess pathological infiltrates in bone marrow trephine cryosections using the monoclonal antibody Ki-67. This method of scoring gave a measure of non-erythroid proliferative activity. RESULTS--Mean Ki-67 positivity in bone marrow infiltrates in 31 low grade B cell lymphomas (Kiel classification) was 0.3% before and 4.7% after treatment, 16.4% in seven high grade B cell lymphomas, and 17.8% in 12 peripheral T cell lymphomas. In 48 cases of NHL, bone marrow cryosections had not been infiltrated, and in all but one case the percentage of Ki-67 positive cells in normal marrow was less than 3%; the remaining case showed coexistent myelodysplasia and 8% bone marrow Ki-67 positivity. In eight cases of common ALL at diagnosis, the mean Ki-67 positivity in marrow cryosections was 24.9%, significantly higher than the 2.4% Ki-67 positivity seen in AML (p < 0.05). One of the two cases of common ALL with less than 1% Ki-67 positivity was refractory to treatment. CONCLUSIONS--Proliferative activity of erythroid elements in the bone marrow varies greatly. Immunostaining of bone marrow cryosections using Ki-67 permits accurate assessment of non-erythroid proliferative activity in lymphomas and leukaemia. High grade B cell lymphomas and peripheral T cell lymphomas invading the marrow have very similar mean proliferative activities. Such levels of proliferation are of the same order as those seen in common ALL, but much higher than those seen in AML.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Hart JS, George SL, Frei E, 3rd, Bodey GP, Nickerson RC, Freireich EJ. Prognotic significance of pretreatment proliferative activity in adult acute leukemia. Cancer. 1977 Apr;39(4):1603–1617. [PubMed]
  • Riccardi A, Danova M, Montecucco C, Ucci G, Cassano E, Giordano M, Mazzini G, Giordano P. Adult acute non-lymphoblastic leukaemia: reliability and prognostic significance of pretreatment bone marrow S-phase size determined by flow cytofluorometry. Scand J Haematol. 1986 Jan;36(1):11–17. [PubMed]
  • Gerdes J, Stein H, Pileri S, Rivano MT, Gobbi M, Ralfkiaer E, Nielsen KM, Pallesen G, Bartels H, Palestro G, et al. Prognostic relevance of tumour-cell growth fraction in malignant non-Hodgkin's lymphomas. Lancet. 1987 Aug 22;2(8556):448–449. [PubMed]
  • Neckers LM, Funkhouser WK, Trepel JB, Cossman J, Gratzner HG. Significant non-S-phase DNA synthesis visualized by flow cytometry in activated and in malignant human lymphoid cells. Exp Cell Res. 1985 Feb;156(2):429–438. [PubMed]
  • Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710–1715. [PubMed]
  • Pileri S, Gerdes J, Rivano M, Tazzari PL, Magnani M, Gobbi M, Stein H. Immunohistochemical determination of growth fractions in human permanent cell lines and lymphoid tumours: a critical comparison of the monoclonal antibodies OKT9 and Ki-67. Br J Haematol. 1987 Mar;65(3):271–276. [PubMed]
  • Hiddemann W, Büchner T, Andreeff M, Wörmann B, Melamed MR, Clarkson BD. Cell kinetics in acute leukemia: a critical reevaluation based on new data. Cancer. 1982 Jul 15;50(2):250–258. [PubMed]
  • Preisler HD, Raza A, Larson RA. Alteration of the proliferative rate of acute myelogenous leukemia cells in vivo in patients. Blood. 1992 Nov 15;80(10):2600–2603. [PubMed]
  • White DM, Smith AG, Whitehouse JM, Smith JL. Peripheral T cell lymphoma: value of bone marrow trephine immunophenotyping. J Clin Pathol. 1989 Apr;42(4):403–408. [PMC free article] [PubMed]
  • Campana D, Janossy G. Proliferation of normal and malignant human immature lymphoid cells. Blood. 1988 May;71(5):1201–1210. [PubMed]
  • Falini B, Canino S, Sacchi S, Ciani C, Martelli MF, Gerdes J, Stein H, Pileri S, Gobbi M, Fagioli M, et al. Immunocytochemical evaluation of the percentage of proliferating cells in pathological bone marrow and peripheral blood samples with the Ki-67 and anti-bromo-deoxyuridine monoclonal antibodies. Br J Haematol. 1988 Jul;69(3):311–320. [PubMed]
  • Scarffe JH, Hann IM, Evans DI, Morris Jones P, Palmer MK, Lilleyman JS, Crowther D. Relationship between the pretreatment proliferative activity of marrow blast cells and prognosis of acute lymphoblastic leukaemia of childhood. Br J Cancer. 1980 May;41(5):764–771. [PMC free article] [PubMed]
  • Dow LW, Chang LJ, Tsiatis AA, Melvin SL, Bowman WP. Relationship of pretreatment lymphoblast proliferative activity and prognosis in 97 children with acute lymphoblastic leukemia. Blood. 1982 Jun;59(6):1197–1202. [PubMed]
  • Chatelain C, Burstein SA, Harker LA. Lithium enhancement of megakaryocytopoiesis in culture: mediation via accessory marrow cells. Blood. 1983 Jul;62(1):172–176. [PubMed]
  • Murphy SB, Dahl GV, George SL, Karas J, Look AT, Simone JV, Mauer AM. Determination of the significance of in vitro blast cell [3H]thymidine labelling indices obtained initially and serially during induction therapy of acute non-lymphocytic leukemia. Leuk Res. 1982;6(5):639–648. [PubMed]
  • Preisler HD, Azarnia N, Raza A, Grunwald H, Vogler R, Browman G, Goldberg J, Chervenick P, Miller K, Brennan J, et al. Relationship between the per cent of marrow cells in S phase and the outcome of remission-induction therapy for acute nonlymphocytic leukaemia. Br J Haematol. 1984 Mar;56(3):399–407. [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...