Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. 1992 Sep 15; 89(18): 8591–8595.
PMCID: PMC49966

De novo generation of neuronal cells from the adult mouse brain.

Abstract

Cells of neuronal morphology, expressing the 150- and 200-kDa neurofilament proteins, were generated in vitro from populations of neural cells dissociated from adult (greater than 60-day-old) mouse brain. Most of these neurons arose from dividing precursors, as demonstrated by the incorporation of [3H]thymidine during the culture period and autoradiography. Neuronal production was optimal under the conditions in which precursors were initially stimulated with basic fibroblast growth factor and then exposed to medium conditioned by an astrocytic cell line, Ast-1, in serum-free medium. Few, if any, neurons arose in control cultures or in cultures kept in serum and fibroblast growth factor. These results suggest that neuronal precursors exist in the adult mammalian brain, but they require discrete epigenetic signals for their proliferation and differentiation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abney ER, Bartlett PP, Raff MC. Astrocytes, ependymal cells, and oligodendrocytes develop on schedule in dissociated cell cultures of embryonic rat brain. Dev Biol. 1981 Apr 30;83(2):301–310. [PubMed]
  • Altman J, Bayer SA. Migration and distribution of two populations of hippocampal granule cell precursors during the perinatal and postnatal periods. J Comp Neurol. 1990 Nov 15;301(3):365–381. [PubMed]
  • Wolswijk G, Noble M. Identification of an adult-specific glial progenitor cell. Development. 1989 Feb;105(2):387–400. [PubMed]
  • Pruss RM, Bartlett PF, Gavrilovic J, Lisak RP, Rattray S. Mitogens for glial cells: a comparison of the response of cultured astrocytes, oligodendrocytes and Schwann cells. Brain Res. 1981 Aug;254(1):19–35. [PubMed]
  • Graziadei GA, Graziadei PP. Neurogenesis and neuron regeneration in the olfactory system of mammals. II. Degeneration and reconstitution of the olfactory sensory neurons after axotomy. J Neurocytol. 1979 Apr;8(2):197–213. [PubMed]
  • Alvarez-Buylla A, Theelen M, Nottebohm F. Birth of projection neurons in the higher vocal center of the canary forebrain before, during, and after song learning. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8722–8726. [PMC free article] [PubMed]
  • Nordeen EJ, Nordeen KW. Estrogen stimulates the incorporation of new neurons into avian song nuclei during adolescence. Brain Res Dev Brain Res. 1989 Sep 1;49(1):27–32. [PubMed]
  • Murphy M, Drago J, Bartlett PF. Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro. J Neurosci Res. 1990 Apr;25(4):463–475. [PubMed]
  • Bartlett PF, Reid HH, Bailey KA, Bernard O. Immortalization of mouse neural precursor cells by the c-myc oncogene. Proc Natl Acad Sci U S A. 1988 May;85(9):3255–3259. [PMC free article] [PubMed]
  • Bernard O, Reid HH, Bartlett PF. Role of the c-myc and the N-myc proto-oncogenes in the immortalization of neural precursors. J Neurosci Res. 1989 Sep;24(1):9–20. [PubMed]
  • Ziller C, Dupin E, Brazeau P, Paulin D, Le Douarin NM. Early segregation of a neuronal precursor cell line in the neural crest as revealed by culture in a chemically defined medium. Cell. 1983 Feb;32(2):627–638. [PubMed]
  • Walsh C, Cepko CL. Widespread dispersion of neuronal clones across functional regions of the cerebral cortex. Science. 1992 Jan 24;255(5043):434–440. [PubMed]
  • Temple S. Division and differentiation of isolated CNS blast cells in microculture. Nature. 1989 Aug 10;340(6233):471–473. [PubMed]
  • Reynolds BA, Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 1992 Mar 27;255(5052):1707–1710. [PubMed]
  • Price J, Thurlow L. Cell lineage in the rat cerebral cortex: a study using retroviral-mediated gene transfer. Development. 1988 Nov;104(3):473–482. [PubMed]
  • Williams BP, Read J, Price J. The generation of neurons and oligodendrocytes from a common precursor cell. Neuron. 1991 Oct;7(4):685–693. [PubMed]
  • Drago J, Murphy M, Carroll SM, Harvey RP, Bartlett PF. Fibroblast growth factor-mediated proliferation of central nervous system precursors depends on endogenous production of insulin-like growth factor I. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2199–2203. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...