• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jclinpathJournal of Clinical PathologyCurrent TOCInstructions for authors
J Clin Pathol. Jul 1991; 44(7): 592–595.
PMCID: PMC496801

Microbiological assay for vitamin B12 performed in 96-well microtitre plates.

Abstract

A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (595K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ANDERSON BB. INVESTIGATIONS INTO THE EUGLENA METHOD FOR THE ASSAY OF THE VITAMIN B12 IN SERUM. J Clin Pathol. 1964 Jan;17:14–26. [PMC free article] [PubMed]
  • SPRAY GH. An improved method for the rapid estimation of vitamin B12 in serum. Clin Sci (Lond) 1955 Nov;14(4):661–667. [PubMed]
  • Temperley IJ, Collery D. The significance of the serum vitamin B 12 estimation in clinical practice. Ir J Med Sci. 1965 Jul-Oct;6(475):317–325. [PubMed]
  • Lindenbaum J. Status of laboratory testing in the diagnosis of megaloblastic anemia. Blood. 1983 Apr;61(4):624–627. [PubMed]
  • Kelleher BP, Scott JM, O'Broin SD. Use of beta-lactamase to hydrolyse interfering antibiotics in vitamin B12 microbiological assay using Lactobacillus leichmannii. Clin Lab Haematol. 1990;12(1):87–95. [PubMed]
  • Mollin DL, Anderson BB, Burman JF. The serum vitamin B12 level: its assay and significance. Clin Haematol. 1976 Oct;5(3):521–546. [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...