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J Clin Pathol. 1991 Jul; 44(7): 592–595.
PMCID: PMC496801

Microbiological assay for vitamin B12 performed in 96-well microtitre plates.

Abstract

A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.

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Selected References

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  • ANDERSON BB. INVESTIGATIONS INTO THE EUGLENA METHOD FOR THE ASSAY OF THE VITAMIN B12 IN SERUM. J Clin Pathol. 1964 Jan;17:14–26. [PMC free article] [PubMed]
  • SPRAY GH. An improved method for the rapid estimation of vitamin B12 in serum. Clin Sci (Lond) 1955 Nov;14(4):661–667. [PubMed]
  • Temperley IJ, Collery D. The significance of the serum vitamin B 12 estimation in clinical practice. Ir J Med Sci. 1965 Jul-Oct;6(475):317–325. [PubMed]
  • Lindenbaum J. Status of laboratory testing in the diagnosis of megaloblastic anemia. Blood. 1983 Apr;61(4):624–627. [PubMed]
  • Kelleher BP, Scott JM, O'Broin SD. Use of beta-lactamase to hydrolyse interfering antibiotics in vitamin B12 microbiological assay using Lactobacillus leichmannii. Clin Lab Haematol. 1990;12(1):87–95. [PubMed]
  • Mollin DL, Anderson BB, Burman JF. The serum vitamin B12 level: its assay and significance. Clin Haematol. 1976 Oct;5(3):521–546. [PubMed]

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