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Proc Natl Acad Sci U S A. 1992 Aug 1; 89(15): 7080–7084.
PMCID: PMC49649

dOct2, a Drosophila Oct transcription factor that functions in yeast.

Abstract

Oct factors are members of the POU family of transcription factors that are shown to play important roles during development in mammals. Here we report the cDNA cloning and expression of a Drosophila Oct transcription factor. Whole mount in situ hybridization experiments revealed that the spatial expression patterns of this gene during embryonic development have not yet been observed for any other gene. In early embryogenesis, its transcripts are transiently expressed as a wide uniform band from 20% to 40% of the egg length, very similar to that of gap genes. This pattern progressively resolves into a series of narrower stripes followed by expression in 14 stripes. Subsequently, transcripts from this gene are expressed in the central nervous system and the brain. When expressed in the yeast Saccharomyces cerevisiae, this Drosophila factor functions as a strong, octamer-dependent activator of transcription. Our data strongly suggest possible functions for the Oct factor in pattern formation in Drosophila that might transcend the boundaries of genetically defined segmentation genes.

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  • Holland PW, Hogan BL. Expression of homeo box genes during mouse development: a review. Genes Dev. 1988 Jul;2(7):773–782. [PubMed]
  • Wright CV. Vertebrate homeobox genes. Curr Opin Cell Biol. 1991 Dec;3(6):976–982. [PubMed]
  • Herr W, Sturm RA, Clerc RG, Corcoran LM, Baltimore D, Sharp PA, Ingraham HA, Rosenfeld MG, Finney M, Ruvkun G, et al. The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes Dev. 1988 Dec;2(12A):1513–1516. [PubMed]
  • Schöler HR. Octamania: the POU factors in murine development. Trends Genet. 1991 Oct;7(10):323–329. [PubMed]
  • Sturm RA, Das G, Herr W. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain. Genes Dev. 1988 Dec;2(12A):1582–1599. [PubMed]
  • Schöler HR, Hatzopoulos AK, Balling R, Suzuki N, Gruss P. A family of octamer-specific proteins present during mouse embryogenesis: evidence for germline-specific expression of an Oct factor. EMBO J. 1989 Sep;8(9):2543–2550. [PMC free article] [PubMed]
  • Müller MM, Ruppert S, Schaffner W, Matthias P. A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells. Nature. 1988 Dec 8;336(6199):544–551. [PubMed]
  • Scheidereit C, Cromlish JA, Gerster T, Kawakami K, Balmaceda CG, Currie RA, Roeder RG. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein. Nature. 1988 Dec 8;336(6199):551–557. [PubMed]
  • Clerc RG, Corcoran LM, LeBowitz JH, Baltimore D, Sharp PA. The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. Genes Dev. 1988 Dec;2(12A):1570–1581. [PubMed]
  • Suzuki N, Rohdewohld H, Neuman T, Gruss P, Schöler HR. Oct-6: a POU transcription factor expressed in embryonal stem cells and in the developing brain. EMBO J. 1990 Nov;9(11):3723–3732. [PMC free article] [PubMed]
  • Monuki ES, Kuhn R, Weinmaster G, Trapp BD, Lemke G. Expression and activity of the POU transcription factor SCIP. Science. 1990 Sep 14;249(4974):1300–1303. [PubMed]
  • Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990 Feb 9;60(3):461–472. [PubMed]
  • Rosner MH, Vigano MA, Ozato K, Timmons PM, Poirier F, Rigby PW, Staudt LM. A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature. 1990 Jun 21;345(6277):686–692. [PubMed]
  • Schöler HR, Ruppert S, Suzuki N, Chowdhury K, Gruss P. New type of POU domain in germ line-specific protein Oct-4. Nature. 1990 Mar 29;344(6265):435–439. [PubMed]
  • Petryniak B, Staudt LM, Postema CE, McCormack WT, Thompson CB. Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1099–1103. [PMC free article] [PubMed]
  • Smith DP, Old RW. Nucleotide sequence of Xenopus laevis Oct-1 cDNA. Nucleic Acids Res. 1990 Jan 25;18(2):369–369. [PMC free article] [PubMed]
  • Calzone FJ, Thézé N, Thiebaud P, Hill RL, Britten RJ, Davidson EH. Developmental appearance of factors that bind specifically to cis-regulatory sequences of a gene expressed in the sea urchin embryo. Genes Dev. 1988 Sep;2(9):1074–1088. [PubMed]
  • Staudt LM, Clerc RG, Singh H, LeBowitz JH, Sharp PA, Baltimore D. Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif. Science. 1988 Jul 29;241(4865):577–580. [PubMed]
  • Vinson CR, LaMarco KL, Johnson PF, Landschulz WH, McKnight SL. In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage. Genes Dev. 1988 Jul;2(7):801–806. [PubMed]
  • Tautz D, Pfeifle C. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma. 1989 Aug;98(2):81–85. [PubMed]
  • Harshman KD, Moye-Rowley WS, Parker CS. Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4. Cell. 1988 Apr 22;53(2):321–330. [PubMed]
  • Ballester R, Michaeli T, Ferguson K, Xu HP, McCormick F, Wigler M. Genetic analysis of mammalian GAP expressed in yeast. Cell. 1989 Nov 17;59(4):681–686. [PubMed]
  • Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Billin AN, Cockerill KA, Poole SJ. Isolation of a family of Drosophila POU domain genes expressed in early development. Mech Dev. 1991 Jun;34(2-3):75–84. [PubMed]
  • Dick T, Yang XH, Yeo SL, Chia W. Two closely linked Drosophila POU domain genes are expressed in neuroblasts and sensory elements. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7645–7649. [PMC free article] [PubMed]
  • He X, Treacy MN, Simmons DM, Ingraham HA, Swanson LW, Rosenfeld MG. Expression of a large family of POU-domain regulatory genes in mammalian brain development. Nature. 1989 Jul 6;340(6228):35–41. [PubMed]
  • Treacy MN, He X, Rosenfeld MG. I-POU: a POU-domain protein that inhibits neuron-specific gene activation. Nature. 1991 Apr 18;350(6319):577–584. [PubMed]
  • Johnson WA, Hirsh J. Binding of a Drosophila POU-domain protein to a sequence element regulating gene expression in specific dopaminergic neurons. Nature. 1990 Feb 1;343(6257):467–470. [PubMed]
  • Bürglin TR, Finney M, Coulson A, Ruvkun G. Caenorhabditis elegans has scores of homoeobox-containing genes. Nature. 1989 Sep 21;341(6239):239–243. [PubMed]
  • Ingraham HA, Chen RP, Mangalam HJ, Elsholtz HP, Flynn SE, Lin CR, Simmons DM, Swanson L, Rosenfeld MG. A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype. Cell. 1988 Nov 4;55(3):519–529. [PubMed]
  • Bodner M, Castrillo JL, Theill LE, Deerinck T, Ellisman M, Karin M. The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein. Cell. 1988 Nov 4;55(3):505–518. [PubMed]
  • Schaffner W. How do different transcription factors binding the same DNA sequence sort out their jobs? Trends Genet. 1989 Feb;5(2):37–39. [PubMed]
  • Hoey T, Dynlacht BD, Peterson MG, Pugh BF, Tjian R. Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID. Cell. 1990 Jun 29;61(7):1179–1186. [PubMed]
  • Gaul U, Jäckle H. Role of gap genes in early Drosophila development. Adv Genet. 1990;27:239–275. [PubMed]
  • Carroll SB. Zebra patterns in fly embryos: activation of stripes or repression of interstripes? Cell. 1990 Jan 12;60(1):9–16. [PubMed]
  • Kornberg T, Sidén I, O'Farrell P, Simon M. The engrailed locus of Drosophila: in situ localization of transcripts reveals compartment-specific expression. Cell. 1985 Jan;40(1):45–53. [PubMed]
  • DiNardo S, Kuner JM, Theis J, O'Farrell PH. Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. Cell. 1985 Nov;43(1):59–69. [PMC free article] [PubMed]
  • Knipple DC, Seifert E, Rosenberg UB, Preiss A, Jäckle H. Spatial and temporal patterns of Krüppel gene expression in early Drosophila embryos. Nature. 1985 Sep 5;317(6032):40–44. [PubMed]
  • Bopp D, Burri M, Baumgartner S, Frigerio G, Noll M. Conservation of a large protein domain in the segmentation gene paired and in functionally related genes of Drosophila. Cell. 1986 Dec 26;47(6):1033–1040. [PubMed]
  • Baker NE. Molecular cloning of sequences from wingless, a segment polarity gene in Drosophila: the spatial distribution of a transcript in embryos. EMBO J. 1987 Jun;6(6):1765–1773. [PMC free article] [PubMed]
  • Gaul U, Seifert E, Schuh R, Jäckle H. Analysis of Krüppel protein distribution during early Drosophila development reveals posttranscriptional regulation. Cell. 1987 Aug 14;50(4):639–647. [PubMed]
  • Frasch M, Hoey T, Rushlow C, Doyle H, Levine M. Characterization and localization of the even-skipped protein of Drosophila. EMBO J. 1987 Mar;6(3):749–759. [PMC free article] [PubMed]
  • Doe CQ, Hiromi Y, Gehring WJ, Goodman CS. Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis. Science. 1988 Jan 8;239(4836):170–175. [PubMed]
  • Kamens J, Brent R. A yeast transcription assay defines distinct rel and dorsal DNA recognition sequences. New Biol. 1991 Oct;3(10):1005–1013. [PubMed]
  • Cheng LZ, Workman JL, Kingston RE, Kelly TJ. Regulation of DNA replication in vitro by the transcriptional activation domain of GAL4-VP16. Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):589–593. [PMC free article] [PubMed]
  • O'Neill EA, Fletcher C, Burrow CR, Heintz N, Roeder RG, Kelly TJ. Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III. Science. 1988 Sep 2;241(4870):1210–1213. [PubMed]
  • Verrijzer CP, Kal AJ, Van der Vliet PC. The DNA binding domain (POU domain) of transcription factor oct-1 suffices for stimulation of DNA replication. EMBO J. 1990 Jun;9(6):1883–1888. [PMC free article] [PubMed]
  • Rosner MH, De Santo RJ, Arnheiter H, Staudt LM. Oct-3 is a maternal factor required for the first mouse embryonic division. Cell. 1991 Mar 22;64(6):1103–1110. [PubMed]

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