• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jclinpathJournal of Clinical PathologyVisit this articleSubmit a manuscriptReceive email alertsContact usBMJ
J Clin Pathol. Mar 1992; 45(3): 228–231.
PMCID: PMC495480

Effect of physical environment on survival of Helicobacter pylori.

Abstract

AIMS: To determine the effects of physical conditions on survival of Helicobacter pylori in aquatic environments. Survival for prolonged time intervals would implicate environmental water as a possible source of infection. METHODS: The effect of ionic strength, pH, urea, protein and composition of incubation atmosphere on the survival of H pylori NCTC 11637 and two clinical isolates (CI 82 and 92) was investigated. RESULTS: H pylori strains survived for longer periods in physiological (0.15M) saline than in 0.05M or 0.6M saline solution. Optimal pH range for survival was between pH 5.8 and 6.9. Addition of urea (final concentration 100 microM/l-1 and 5 mM/l-1) to neutral unbuffered 0.15M saline resulted in a reduction in survival; addition of bovine serum albumin (1%) or gelatin (1%) resulted in variable survival times compared with saline alone. Incubation in a microaerobic gas mixture prolonged survival compared with incubation in air. CONCLUSION: H pylori survival in water over a prolonged period is possible for a range of physical variables. The results indicate that H pylori could survive in environmental water which may thus act as a potential reservoir of infection.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (641K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Berkowicz J, Lee A. Person-to-person transmission of Campylobacter pylori. Lancet. 1987 Sep 19;2(8560):680–681. [PubMed]
  • Klein PD, Graham DY, Gaillour A, Opekun AR, Smith EO. Water source as risk factor for Helicobacter pylori infection in Peruvian children. Gastrointestinal Physiology Working Group. Lancet. 1991 Jun 22;337(8756):1503–1506. [PubMed]
  • West AP, Millar MR, Tompkins DS. Survival of Helicobacter pylori in water and saline. J Clin Pathol. 1990 Jul;43(7):609–609. [PMC free article] [PubMed]
  • Tompkins DS, Nehaul BB, Smith CA, Cooke EM. Evaluation of the Phadebact Gonococcus Test in the identification of Neisseria gonorrhoeae in a routine diagnostic laboratory. J Clin Pathol. 1981 Oct;34(10):1106–1109. [PMC free article] [PubMed]
  • Tompkins DS, West AP. Campylobacter pylori, acid, and bile. J Clin Pathol. 1987 Nov;40(11):1387–1387. [PMC free article] [PubMed]
  • Morgan DR, Freedman R, Depew CE, Kraft WG. Growth of Campylobacter pylori in liquid media. J Clin Microbiol. 1987 Nov;25(11):2123–2125. [PMC free article] [PubMed]
  • Dick JD. Helicobacter (Campylobacter) pylori: a new twist to an old disease. Annu Rev Microbiol. 1990;44:249–269. [PubMed]
  • Mooney C, Munster DJ, Bagshaw PF, Allardyce RA. Helicobacter pylori acid resistance. Lancet. 1990 May 19;335(8699):1232–1232. [PubMed]
  • Rathbone BJ, West AP, Wyatt JI, Johnson AW, Tompkins DS, Heatley RV. Campylobacter pyloridis, urease, and gastric ulcers. Lancet. 1986 Aug 16;2(8503):400–401. [PubMed]
  • Coudron PE, Kirby DF. Comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of Campylobacter pylori. J Clin Microbiol. 1989 Jul;27(7):1527–1530. [PMC free article] [PubMed]
  • Oudbier JH, Langenberg W, Rauws EA, Bruin-Mosch C. Genotypical variation of Campylobacter pylori from gastric mucosa. J Clin Microbiol. 1990 Mar;28(3):559–565. [PMC free article] [PubMed]
  • Hazell SL, Graham DY. Unsaturated fatty acids and viability of Helicobacter (Campylobacter) pylori. J Clin Microbiol. 1990 May;28(5):1060–1061. [PMC free article] [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...