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J Clin Pathol. Aug 1981; 34(8): 851–858.
PMCID: PMC493957

Human intestinal mucosal mast cells: evaluation of fixation and staining techniques.

Abstract

The staining properties of tissue mast cells are influenced by the method of fixation. Differences in fixation and staining techniques may explain the contradictory results in the published reports on the number of human mucosal mast cells (MMC) in the gastrointestinal mucosa in health and disease. We have examined the influence of fixatives on the staining properties of human MMC in operative biopsy specimens of human jejunum. Specimens were divided into pieces, each of which was fixed in one of the following fixatives: Carnoy's, basic lead acetate (BLA), Baker's, Bouin's, isotonic formol-acetic-acid (IFAA), 10% neutral buffered formalin, formol sublimate, and formol saline. Thereafter, tissues were paraffin-embedded and 5 micron sections were cut and stained with either astra-blue/safranin pH 0.3, or toluidine blue pH 0.5. Counts of the number of MMC/mm2 were obtained for each fixation method. The results show a critical influence of the fixative on the number of mast cells identified after staining. For example with astra-blue/safranin the mean MMC/mm2 count was 40 in formol-saline-fixed specimens, and 268 in Carnoy's-fixed specimens. In biopsies fixed with formalin-based fixatives, mast cells were more readily stained with toluidine blue. It is recommended that Carnoy's or BLA be used as the fixative for any light microscopic study of human MMC.

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Selected References

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  • Miller HR, Walshaw R. Immune reactions in mucous membranes. IV. Histochemistry of intestinal mast cells during helminth expulsion in the rat. Am J Pathol. 1972 Oct;69(1):195–208. [PMC free article] [PubMed]
  • Enerbäck L. Mast cells in rat gastrointestinal mucosa. 2. Dye-binding and metachromatic properties. Acta Pathol Microbiol Scand. 1966;66(3):303–312. [PubMed]
  • BLOOM G, KELLY JW. The copper phthalocyanin dye "Astrablau" and its staining properties, especially the staining of mast cells. Z Zellforch Microsk Anat Histochem. 1960;2:48–57. [PubMed]
  • Mayrhofer G. Thymus-dependent and thymus-independent subpopulations of intestinal intraepithelial lymphocytes: a granular subpopulation of probable bone marrow origin and relationship to mucosal mast cells. Blood. 1980 Mar;55(3):532–535. [PubMed]
  • Guy-Grand D, Griscelli C, Vassalli P. The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978 Dec 1;148(6):1661–1677. [PMC free article] [PubMed]
  • Brandtzaeg P, Baklien K, Fausa O, Hoel PS. Immunohistochemical characterization of local immunoglobulin formation in ulcerative colitis. Gastroenterology. 1974 Jun;66(6):1123–1136. [PubMed]
  • Batcup G, Spitz L. A histopathological study of gastric mucosal biopsies in infantile hypertrophic pyloric stenosis. J Clin Pathol. 1979 Jun;32(6):625–628. [PMC free article] [PubMed]
  • Takahashi T, Shimazu H, Yamagishi T, Tani M. G-cell populations in resected stomachs from gastric and duodenal ulcer patients. Gastroenterology. 1980 Mar;78(3):498–504. [PubMed]
  • Sjölund K, Alumets J, Berg NO, Håkanson R, Sundler F. Duodenal endocrine cells in adult coeliac disease. Gut. 1979 Jul;20(7):547–552. [PMC free article] [PubMed]
  • Selbekk BH, Flaten O, Hanssen LE. The in vitro effect of neurotensin on human jejunal mast cells. Scand J Gastroenterol. 1980;15(4):457–460. [PubMed]
  • Dollberg L, Gurevitz M, Freier S. Gastrointestinal mast cells in health, and in coeliac disease and other conditions. Arch Dis Child. 1980 Sep;55(9):702–705. [PMC free article] [PubMed]
  • Woodbury RG, Gruzenski GM, Lagunoff D. Immunofluorescent localization of a serine protease in rat small intestine. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2785–2789. [PMC free article] [PubMed]
  • Tas J, Berndsen RG. Does heparin occur in mucosal mast cells of the rat small intestine? J Histochem Cytochem. 1977 Sep;25(9):1058–1062. [PubMed]
  • Piris J, Thomas ND. A quantitative study of the influence of fixation on immunoperoxidase staining of rectal mucosal plasma cells. J Clin Pathol. 1980 Apr;33(4):361–364. [PMC free article] [PubMed]

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