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Journal List > J Clin Pathol > v.22(2); Mar 1969

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J Clin Pathol. 1969 March; 22(2): 144–151.
PMCID: PMC474023
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Activation of serum 5′ nucleotidase by magnesium ions and its diagnostic applications
A. Belfield and D. M. Goldberg
Department of Chemical Pathology, The Royal Hospital, Sheffield, 1
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Abstract
Magnesium ions increase the hydrolysis of adenosine 5′ monophosphate (5′ AMP) by human serum at pH 7·9 but not at pH 9·3. The additional hydrolysis at pH 7·9 is predominantly due to increased activity of the specific phosphatase 5′ nucleotidase (5Nase). This increase is proportional to enzyme concentration and has been employed as a measure of 5Nase activity in a sensitive micro-estimation.
The normal range for serum 5Nase activity by this technique was 0 to 20 mIU/ml. In a series of over 200 patients, raised values were found frequently in hepatobiliary disease and infrequently in bone disease. Assay of 5Nase activity gave a more reliable indication of the source of raised serum alkaline phosphatase than isoenzyme electrophoresis in agar gel. The correlation between activities of the two enzymes was low in bone disease generally, and fairly good in hepatobiliary disease. The closest correlation was found in patients with parenchymal liver disease.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • AHMED Z, REIS JL. The activation and inhibition of 5-nucleotidase. Biochem J. 1958 Jul;69(3):386–387. [PubMed]
  • BARDAWILL C, CHANG C. SERUM LACTIC DEHYDROGENASE, LEUCINE AMINOPEPTIDASE AND 5-NUCLEOTIDASE ACTIVITIES: OBSERVATION IN PATIENTS WITH CARCINOMA OF THE PANCREAS AND HEPATOBILIARY DISEASE. Can Med Assoc J. 1963 Oct 12;89:755–761. [PubMed]
  • BARKA T. Studies on acid phosphatases. II. Chromatographic separation of acid phosphatases of rat liver. J Histochem Cytochem. 1961 Sep;9:564–571. [PubMed]
  • Belfield A, Goldberg DM. Inhibition of the nucleotidase effect of alkaline phosphatase by beta-glycerophosphate. Nature. 1968 Jul 6;219(5149):73–75. [PubMed]
  • DIXON TF, PURDOM M. Serum 5-nucleotidase. J Clin Pathol. 1954 Nov;7(4):341–343. [PubMed]
  • Dymling JF. Separation of serum and placental alkaline phosphatase by agarose gel electrophoresis and Sephadex chromatography. Scand J Clin Lab Invest. 1966;18(2):129–133. [PubMed]
  • HAIJE WG, DE JONG M. ISO-ENZYME PATTERNS OF SERUM ALKALINE PHOSPHATASE IN AGAR-GEL ELECTROPHORESIS AND THEIR CLINICAL SIGNIFICANCE. Clin Chim Acta. 1963 Jul;8:620–623. [PubMed]
  • Hardonk MJ, Koudstaal J. 5'-nucleotidase. II. The significance of 5'-nucleotidase in the metabolism of nucleotides studied by histochemical and biochemical methods. Histochemie. 1968;12(1):18–28. [PubMed]
  • KOWLESSAR OD, HAEFFNER LJ, RILEY EM, SLEISENGER MH. Comparative study of serum leucine aminopeptidase, 5-nucleotidase and non-specific alkaline phosphatase in diseases affecting the pancreas, hepatobiliary tree and bone. Am J Med. 1961 Aug;31:231–237. [PubMed]
  • REIS JL. The specificity of phospho-monoesterases in human tissues. Biochem J. 1951 May;48(5):548–551. [PubMed]
  • SCHWARTZ MK, BODANSKY O. PROPERTIES OF ACTIVITY OF 5'-NUCLEOTIDASE IN HUMAN SERUM, AND APPLICATIONS IN DIAGNOSIS. Am J Clin Pathol. 1964 Dec;42:572–580. [PubMed]
  • VINNIK IE, KERN F, Jr, CORLEY WD. SERUM 5-NUCLEOTIDASE AND PERICHOLANGITIS IN PATIENTS WITH CHRONIC ULCERATIVE COLITIS. Gastroenterology. 1963 Oct;45:492–498. [PubMed]
  • YOUNG II. Serum 5-nucleotidase; characterization and evaluation in disease states. Ann N Y Acad Sci. 1958 Oct 13;75(1):357–362. [PubMed]
  • WACHSTEIN M, SIGISMONDI R. 5-Nucleotidase activity and adenosine triphosphatase activity in human serums. Am J Clin Pathol. 1958 Dec;30(6):523–527. [PubMed]
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