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Proc Natl Acad Sci U S A. 1993 Aug 15; 90(16): 7598–7602.

Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons.


A diacylglycerol kinase cDNA was isolated from a rat brain cDNA library. This cDNA encoded an 801-amino acid protein of 90,287 Da. This 90-kDa diacylglycerol kinase showed 58% identity in deduced amino acid sequence with a previously isolated rat 80-kDa diacylglycerol kinase. EF-hand motifs, cysteine-rich zinc-finger-like sequences, and putative ATP-binding sites were all conserved between the two kinase species. However, mRNA encoding the 90-kDa kinase was confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle. Further, the 90-kDa kinase was found to exhibit high phosphorylation activity for long-chain diacylglycerols and was mainly associated with the membrane fraction when the cDNA was transfected into COS-7 cells.

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  • Bishop WR, Ganong BR, Bell RM. Attenuation of sn-1,2-diacylglycerol second messengers by diacylglycerol kinase. Inhibition by diacylglycerol analogs in vitro and in human platelets. J Biol Chem. 1986 May 25;261(15):6993–7000. [PubMed]
  • Kanoh H, Yamada K, Sakane F. Diacylglycerol kinase: a key modulator of signal transduction? Trends Biochem Sci. 1990 Feb;15(2):47–50. [PubMed]
  • de Chaffoy de Courcelles DC, Roevens P, Van Belle H. R 59 022, a diacylglycerol kinase inhibitor. Its effect on diacylglycerol and thrombin-induced C kinase activation in the intact platelet. J Biol Chem. 1985 Dec 15;260(29):15762–15770. [PubMed]
  • Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984 Apr 19;308(5961):693–698. [PubMed]
  • Inoue H, Yoshioka T, Hotta Y. Diacylglycerol kinase defect in a Drosophila retinal degeneration mutant rdgA. J Biol Chem. 1989 Apr 5;264(10):5996–6000. [PubMed]
  • Kanoh H, Kondoh H, Ono T. Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies. J Biol Chem. 1983 Feb 10;258(3):1767–1774. [PubMed]
  • Sakane F, Yamada K, Kanoh H, Yokoyama C, Tanabe T. Porcine diacylglycerol kinase sequence has zinc finger and E-F hand motifs. Nature. 1990 Mar 22;344(6264):345–348. [PubMed]
  • Goto K, Watanabe M, Kondo H, Yuasa H, Sakane F, Kanoh H. Gene cloning, sequence, expression and in situ localization of 80 kDa diacylglycerol kinase specific to oligodendrocyte of rat brain. Brain Res Mol Brain Res. 1992 Nov;16(1-2):75–87. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
  • Takebe Y, Seiki M, Fujisawa J, Hoy P, Yokota K, Arai K, Yoshida M, Arai N. SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol Cell Biol. 1988 Jan;8(1):466–472. [PMC free article] [PubMed]
  • Sakane F, Imai S, Yamada K, Kanoh H. The regulatory role of EF-hand motifs of pig 80K diacylglycerol kinase as assessed using truncation and deletion mutants. Biochem Biophys Res Commun. 1991 Dec 31;181(3):1015–1021. [PubMed]
  • Okayama H, Kawaichi M, Brownstein M, Lee F, Yokota T, Arai K. High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells. Methods Enzymol. 1987;154:3–28. [PubMed]
  • Sakane F, Yamada K, Imai S, Kanoh H. Porcine 80-kDa diacylglycerol kinase is a calcium-binding and calcium/phospholipid-dependent enzyme and undergoes calcium-dependent translocation. J Biol Chem. 1991 Apr 15;266(11):7096–7100. [PubMed]
  • Fabiato A, Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 1979;75(5):463–505. [PubMed]
  • MacDonald ML, Mack KF, Williams BW, King WC, Glomset JA. A membrane-bound diacylglycerol kinase that selectively phosphorylates arachidonoyl-diacylglycerol. Distinction from cytosolic diacylglycerol kinase and comparison with the membrane-bound enzyme from Escherichia coli. J Biol Chem. 1988 Jan 25;263(3):1584–1592. [PubMed]
  • Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. [PubMed]
  • Lemaitre RN, King WC, MacDonald ML, Glomset JA. Distribution of distinct arachidonoyl-specific and non-specific isoenzymes of diacylglycerol kinase in baboon (Papio cynocephalus) tissues. Biochem J. 1990 Feb 15;266(1):291–299. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Kaiser ET, Kézdy FJ. Peptides with affinity for membranes. Annu Rev Biophys Biophys Chem. 1987;16:561–581. [PubMed]
  • Kalmar GB, Kay RJ, Lachance A, Aebersold R, Cornell RB. Cloning and expression of rat liver CTP: phosphocholine cytidylyltransferase: an amphipathic protein that controls phosphatidylcholine synthesis. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6029–6033. [PMC free article] [PubMed]
  • MacDonald ML, Mack KF, Richardson CN, Glomset JA. Regulation of diacylglycerol kinase reaction in Swiss 3T3 cells. Increased phosphorylation of endogenous diacylglycerol and decreased phosphorylation of didecanoylglycerol in response to platelet-derived growth factor. J Biol Chem. 1988 Jan 25;263(3):1575–1583. [PubMed]
  • Young WS., 3rd Expression of three (and a putative four) protein kinase C genes in brains of rat and rabbit. J Chem Neuroanat. 1988 Jul-Aug;1(4):177–194. [PubMed]
  • Ross CA, MacCumber MW, Glatt CE, Snyder SH. Brain phospholipase C isozymes: differential mRNA localizations by in situ hybridization. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2923–2927. [PMC free article] [PubMed]
  • Mahan LC, Burch RM, Monsma FJ, Jr, Sibley DR. Expression of striatal D1 dopamine receptors coupled to inositol phosphate production and Ca2+ mobilization in Xenopus oocytes. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2196–2200. [PMC free article] [PubMed]

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