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Proc Natl Acad Sci U S A. Aug 1, 1993; 90(15): 7181–7185.
PMCID: PMC47100

Hepsin, a putative cell-surface serine protease, is required for mammalian cell growth.

Abstract

Hepsin was previously identified as a putative cell-surface serine protease. When hepatoma cells were treated with anti-hepsin antibodies, their growth was substantially arrested, suggesting the requirement of hepsin molecules present at the cell surface for normal cell growth. This was further supported by a gross inhibition of cell growth with hepsin-specific antisense oligonucleotides. Upon treatment of cells with antisense oligonucleotides, rapid reduction in cellular hepsin was observed. This reduction in cellular hepsin levels was accompanied by drastic morphological changes. Various tissues in the developing mouse embryo showed greatly elevated hepsin levels in regions of active proliferation. These results indicate that hepsin plays an essential role in cell growth and maintenance of cell morphology.

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  • Bond JS. Plasma membrane proteases: introductory remarks. Biomed Biochim Acta. 1991;50(4-6):775–780. [PubMed]
  • Tsuji A, Torres-Rosado A, Arai T, Le Beau MM, Lemons RS, Chou SH, Kurachi K. Hepsin, a cell membrane-associated protease. Characterization, tissue distribution, and gene localization. J Biol Chem. 1991 Sep 5;266(25):16948–16953. [PubMed]
  • Leytus SP, Loeb KR, Hagen FS, Kurachi K, Davie EW. A novel trypsin-like serine protease (hepsin) with a putative transmembrane domain expressed by human liver and hepatoma cells. Biochemistry. 1988 Feb 9;27(3):1067–1074. [PubMed]
  • Yoshitake S, Schach BG, Foster DC, Davie EW, Kurachi K. Nucleotide sequence of the gene for human factor IX (antihemophilic factor B). Biochemistry. 1985 Jul 2;24(14):3736–3750. [PubMed]
  • MacNab GM, Alexander JJ, Lecatsas G, Bey EM, Urbanowicz JM. Hepatitis B surface antigen produced by a human hepatoma cell line. Br J Cancer. 1976 Nov;34(5):509–515. [PMC free article] [PubMed]
  • Stein CA, Cohen JS. Oligodeoxynucleotides as inhibitors of gene expression: a review. Cancer Res. 1988 May 15;48(10):2659–2668. [PubMed]
  • Reed JC, Stein C, Subasinghe C, Haldar S, Croce CM, Yum S, Cohen J. Antisense-mediated inhibition of BCL2 protooncogene expression and leukemic cell growth and survival: comparisons of phosphodiester and phosphorothioate oligodeoxynucleotides. Cancer Res. 1990 Oct 15;50(20):6565–6570. [PubMed]
  • Scott GK, Seow HF, Tse CA. Investigations into the nature of growth-related proteolysis in human fibroblasts. Biochim Biophys Acta. 1989 Feb 9;1010(2):160–165. [PubMed]
  • Steven FS, Griffin MM. Inhibitors of guanidinobenzoatase and their possible role in cell migration. Biol Chem Hoppe Seyler. 1988 May;369 (Suppl):137–143. [PubMed]
  • Tanaka K, Nakamura T, Ichihara A. A unique trypsin-like protease associated with plasma membranes of rat liver. J Biol Chem. 1986 Feb 25;261(6):2610–2615. [PubMed]
  • Satoh M, Yokosawa H, Ishii S. Membrane-bound trypsin-like enzyme functioning in degradation of dynorphin in neuroblastoma cells. Purification and characterization. J Biochem. 1988 Mar;103(3):493–498. [PubMed]
  • Scott GK, Seow HF. Further evidence for a cell surface proteinase essential to the growth of cultured fibroblasts. Exp Cell Res. 1985 May;158(1):41–52. [PubMed]
  • Chen JM, Chen WT. Fibronectin-degrading proteases from the membranes of transformed cells. Cell. 1987 Jan 30;48(2):193–203. [PubMed]
  • Aoyama A, Chen WT. A 170-kDa membrane-bound protease is associated with the expression of invasiveness by human malignant melanoma cells. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8296–8300. [PMC free article] [PubMed]
  • Valinsky JE, Le Douarin NM. Production of plasminogen activator by migrating cephalic neural crest cells. EMBO J. 1985 Jun;4(6):1403–1406. [PMC free article] [PubMed]
  • Kido H, Fukutomi A, Katunuma N. A novel membrane-bound serine esterase in human T4(+)-lymphocytes is a binding protein of envelope glycoprotein gp120 of HIV-1. Biomed Biochim Acta. 1991;50(4-6):781–789. [PubMed]
  • Delmas B, Gelfi J, L'Haridon R, Vogel LK, Sjöström H, Norén O, Laude H. Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV. Nature. 1992 Jun 4;357(6377):417–420. [PubMed]
  • Yeager CL, Ashmun RA, Williams RK, Cardellichio CB, Shapiro LH, Look AT, Holmes KV. Human aminopeptidase N is a receptor for human coronavirus 229E. Nature. 1992 Jun 4;357(6377):420–422. [PubMed]

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