• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. May 15, 1993; 90(10): 4557–4561.
PMCID: PMC46551

Methylation induced premeiotically in Ascobolus: coextension with DNA repeat lengths and effect on transcript elongation.

Abstract

The effect of duplications of gene fragments on the triggering of DNA methylation induced premeiotically (MIP) was studied in the sexual progeny of strains harboring, in addition to the resident met2 gene, a fragment of this gene inserted at an ectopic position. Cytosine methylation of the resident gene was checked for each of the eight duplications tested. Methylation was always found and it was coextensive with the length of the duplications. Silencing of the resident gene was triggered by duplications of segments corresponding to the region 5' to the open reading frame, to only the open reading frame, or to segments beginning 0.87-1.2 kb downstream from the transcription start. Silencing was accompanied by either the absence of transcripts or the presence of truncated transcripts, which suggests that methylation acts on transcript elongation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Faugeron G, Goyon C, Grégoire A. Stable allele replacement and unstable non-homologous integration events during transformation of Ascobolus immersus. Gene. 1989 Mar 15;76(1):109–119. [PubMed]
  • Goyon C, Faugeron G. Targeted transformation of Ascobolus immersus and de novo methylation of the resulting duplicated DNA sequences. Mol Cell Biol. 1989 Jul;9(7):2818–2827. [PMC free article] [PubMed]
  • Faugeron G, Rhounim L, Rossignol JL. How does the cell count the number of ectopic copies of a gene in the premeiotic inactivation process acting in Ascobolus immersus? Genetics. 1990 Mar;124(3):585–591. [PMC free article] [PubMed]
  • Rhounim L, Rossignol JL, Faugeron G. Epimutation of repeated genes in Ascobolus immersus. EMBO J. 1992 Dec;11(12):4451–4457. [PMC free article] [PubMed]
  • Selker EU, Cambareri EB, Jensen BC, Haack KR. Rearrangement of duplicated DNA in specialized cells of Neurospora. Cell. 1987 Dec 4;51(5):741–752. [PubMed]
  • Selker EU, Garrett PW. DNA sequence duplications trigger gene inactivation in Neurospora crassa. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6870–6874. [PMC free article] [PubMed]
  • Cambareri EB, Jensen BC, Schabtach E, Selker EU. Repeat-induced G-C to A-T mutations in Neurospora. Science. 1989 Jun 30;244(4912):1571–1575. [PubMed]
  • Fincham JR. Generation of new functional mutant alleles by premeiotic disruption of the Neurospora crassa am gene. Curr Genet. 1990 Dec;18(5):441–445. [PubMed]
  • RIZET G, ENGELMANN N, LEFORT C, LISSOUBA P, MOUSSEAU J. [On an Ascomycete of interest for the study of certain aspects of the problem of gene structure]. C R Hebd Seances Acad Sci. 1960 Mar 14;250:2050–2052. [PubMed]
  • Goyon C, Faugeron G, Rossignol JL. Molecular cloning and characterization of the met2 gene from Ascobolus immersus. Gene. 1988 Mar 31;63(2):297–308. [PubMed]
  • Struhl K, Davis RW. Transcription of the his3 gene region in Saccharomyces cerevisiae. J Mol Biol. 1981 Nov 5;152(3):535–552. [PubMed]
  • Hynes MJ, Corrick CM, King JA. Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Mol Cell Biol. 1983 Aug;3(8):1430–1439. [PMC free article] [PubMed]
  • Nelson M, McClelland M. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases. Nucleic Acids Res. 1991 Apr 25;19 (Suppl):2045–2071. [PMC free article] [PubMed]
  • Fidel S, Doonan JH, Morris NR. Aspergillus nidulans contains a single actin gene which has unique intron locations and encodes a gamma-actin. Gene. 1988 Oct 30;70(2):283–293. [PubMed]
  • Foss EJ, Garrett PW, Kinsey JA, Selker EU. Specificity of repeat-induced point mutation (RIP) in Neurospora: sensitivity of non-Neurospora sequences, a natural diverged tandem duplication, and unique DNA adjacent to a duplicated region. Genetics. 1991 Apr;127(4):711–717. [PMC free article] [PubMed]
  • Keshet I, Yisraeli J, Cedar H. Effect of regional DNA methylation on gene expression. Proc Natl Acad Sci U S A. 1985 May;82(9):2560–2564. [PMC free article] [PubMed]
  • Deobagkar DD, Liebler M, Graessmann M, Graessmann A. Hemimethylation of DNA prevents chromatin expression. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1691–1695. [PMC free article] [PubMed]
  • Iguchi-Ariga SM, Schaffner W. CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation. Genes Dev. 1989 May;3(5):612–619. [PubMed]
  • Kovesdi I, Reichel R, Nevins JR. Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2180–2184. [PMC free article] [PubMed]
  • Watt F, Molloy PL. Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter. Genes Dev. 1988 Sep;2(9):1136–1143. [PubMed]
  • Boyes J, Bird A. Repression of genes by DNA methylation depends on CpG density and promoter strength: evidence for involvement of a methyl-CpG binding protein. EMBO J. 1992 Jan;11(1):327–333. [PMC free article] [PubMed]
  • Lewis JD, Meehan RR, Henzel WJ, Maurer-Fogy I, Jeppesen P, Klein F, Bird A. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell. 1992 Jun 12;69(6):905–914. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...