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Thorax. Jul 1983; 38(7): 516–522.
PMCID: PMC459598

Histamine dose-response curves in asthma: reproducibility and sensitivity of different indices to assess response.

Abstract

In 18 clinically stable asthmatic patients histamine inhalation challenges were performed with a Wright's nebuliser and tidal volume breathing for two minutes on two to four occasions for each subject at a maximum interval of two weeks. The response was measured in terms of specific lung conductance (sGL) by the subtraction technique, maximum partial and maximum complete expiratory flow at 40% and 50% of vital capacity respectively (Vmax40p and Vmax50c), and FEV1 from the maximum flow-volume curve. Dose-response curves were analysed for (1) provocative concentration (PC) of histamine causing a 20% fall in FEV1 and a 40% change in the other measurements; (2) threshold concentration (TC)--the concentration at which changes in the measurement exceed 2 SD from control values; (3) reactivity (R)--the slope of the dose-response curve beyond TC. We found that PC20-FEV1 was the most reproducible index, the 95% confidence intervals based on a single determination being +/- 1.6 single two-fold concentration difference. PC20-FEV1 was more reproducible than PC values for other measurements and more reproducible than any of the TC values. The 95% confidence intervals based on a single determination of R varied from +/- 52% to +/- 74% change/log histamine concentration. Both sGL and Vmax40p detected the bronchoconstrictor response assessed by PC and TC at a significantly lower histamine concentration than FEV1 (p less than 0.01 and p less than 0.05 respectively). PC and TC results showed a significant correlation, but neither were correlated with R.

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Selected References

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  • Curry JJ. THE ACTION OF HISTAMINE ON THE RESPIRATORY TRACT IN NORMAL AND ASTHMATIC SUBJECTS. J Clin Invest. 1946 Nov;25(6):785–791. [PMC free article] [PubMed]
  • Chai H, Farr RS, Froehlich LA, Mathison DA, McLean JA, Rosenthal RR, Sheffer AL, Spector SL, Townley RG. Standardization of bronchial inhalation challenge procedures. J Allergy Clin Immunol. 1975 Oct;56(4):323–327. [PubMed]
  • Cockcroft DW, Killian DN, Mellon JJ, Hargreave FE. Bronchial reactivity to inhaled histamine: a method and clinical survey. Clin Allergy. 1977 May;7(3):235–243. [PubMed]
  • Juniper EF, Frith PA, Dunnett C, Cockcroft DW, Hargreave FE. Reproducibility and comparison of responses to inhaled histamine and methacholine. Thorax. 1978 Dec;33(6):705–710. [PMC free article] [PubMed]
  • Ryan G, Dolovich MB, Roberts RS, Frith PA, Juniper EF, Hargreave FE, Newhouse MT. Standardization of inhalation provocation tests: two techniques of aerosol generation and inhalation compared. Am Rev Respir Dis. 1981 Feb;123(2):195–199. [PubMed]
  • Grimaud C, Fondarai J, Aldebert J, Charpin J. Etude de la reproductibilité du test à l'acétylcholine. Poumon Coeur. 1967;23(2):147–153. [PubMed]
  • MILIC-EMILI J, MEAD J, TURNER JM, GLAUSER EM. IMPROVED TECHNIQUE FOR ESTIMATING PLEURAL PRESSURE FROM ESOPHAGEAL BALLOONS. J Appl Physiol. 1964 Mar;19:207–211. [PubMed]
  • GOLDMAN HI, BECKLAKE MR. Respiratory function tests; normal values at median altitudes and the prediction of normal results. Am Rev Tuberc. 1959 Apr;79(4):457–467. [PubMed]
  • Cartier A, Malo JL, Bégin P, Sestier M, Martin RR. Time course of the bronchoconstriction induced by inhaled histamine and methacholine. J Appl Physiol Respir Environ Exerc Physiol. 1983 Mar;54(3):821–826. [PubMed]
  • Orehek J, Kabondo P, Charpin J, Grimaud C. Mesure spirométrique de la réponse bronchique au carbachol dans l'asthme. Bull Eur Physiopathol Respir. 1978 Sep-Oct;14(5):493–502. [PubMed]
  • Beaupré A, Malo JL. Histamine dose-response curves in asthma: relevance of the distinction between PC20 and reactivity in characterising clinical state. Thorax. 1981 Oct;36(10):731–736. [PMC free article] [PubMed]
  • Orehek J, Gayrard P, Smith AP, Grimaud C, Charpin J. Airway response to carbachol in normal and asthmatic subjects: distinction between bronchial sensitivity and reactivity. Am Rev Respir Dis. 1977 Jun;115(6):937–943. [PubMed]
  • Eiser NM, MacRae KD, Guz A. Evaluation and expression of bronchial provocation tests. Bull Eur Physiopathol Respir. 1981;17(3):427–440. [PubMed]
  • Orehek J. Relations dose-reponse et tests pharmacologiques de provocation bronchique. Bull Eur Physiopathol Respir. 1981;17(3):329–332. [PubMed]
  • Bouhuys A, Hunt VR, Kim BM, Zapletal A. Maximum expiratory flow rates in induced bronchoconstriction in man. J Clin Invest. 1969 Jun;48(6):1159–1168. [PMC free article] [PubMed]
  • Fish JE, Kelly JF. Measurements of responsiveness in bronchoprovocation testing. J Allergy Clin Immunol. 1979 Dec;64(6 Pt 2):592–596. [PubMed]
  • Orehek J, Nicoli MM, Delpierre S, Beaupre A. Influence of the previous deep inspiration on the spirometric measurement of provoked bronchoconstriction in asthma. Am Rev Respir Dis. 1981 Mar;123(3):269–272. [PubMed]
  • Juniper EF, Frith PA, Hargreave FE. Airway responsiveness to histamine and methacholine: relationship to minimum treatment to control symptoms of asthma. Thorax. 1981 Aug;36(8):575–579. [PMC free article] [PubMed]
  • Malo JL, Filiatrault S, Martin RR. Bronchial responsiveness to inhaled methacholine in young asymptomatic smokers. J Appl Physiol Respir Environ Exerc Physiol. 1982 Jun;52(6):1464–1470. [PubMed]
  • Ryan G, Dolovich MB, Obminski G, Cockcroft DW, Juniper E, Hargreave FE, Newhouse MT. Standardization of inhalation provocation tests: influence of nebulizer output, particle size, and method of inhalation. J Allergy Clin Immunol. 1981 Feb;67(2):156–161. [PubMed]
  • Orehek J, Gayrard P. Les tests de provocation bronchique non-spécifiques dans l'asthme. Bull Eur Physiopathol Respir. 1976 Jul-Aug;12(4):565–598. [PubMed]
  • Hargreave FE, Ryan G, Thomson NC, O'Byrne PM, Latimer K, Juniper EF, Dolovich J. Bronchial responsiveness to histamine or methacholine in asthma: measurement and clinical significance. J Allergy Clin Immunol. 1981 Nov;68(5):347–355. [PubMed]
  • Michoud MC, Ghezzo H, Amyot R. A comparison of pulmonary function tests used for bronchial challenges. Bull Eur Physiopathol Respir. 1982 Jul-Aug;18(4):609–621. [PubMed]

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