• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jan 15, 1993; 90(2): 467–471.

Interaction between a coating-borne peptide of the Brassica pollen grain and stigmatic S (self-incompatibility)-locus-specific glycoproteins.


Methods are described for the removal of the sporophytic pollen grain coating of Brassica oleracea and for the isolation of coat polypeptides. The coat contains a small number of proteins ranging from 6 to 45 kDa. Many of the larger proteins are glycosylated, while all carry high positive charges resulting in pI values from 8.5 to 11. Polypeptides with pI values of 9.5, 9.0, and 8.5 possess strong esterase activity. No major differences could be detected in either pI values or molecular masses of pollen-coating polypeptides from grains carrying different sporophytically expressed S (self-incompatibility) alleles. Mixing pollen coat proteins with stigmatic extracts results in a conspicuous binding interaction involving female S-locus-specific and perhaps S-locus-related glycoproteins. This interaction, which is reversed by heating in the presence of SDS, results in an apparent charge shift of the female glycoprotein(s) of up to 2 pI units. The male participant in this interaction has been isolated by using a combination of fast protein liquid chromatography and reverse-phase HPLC and was shown to be a 7-kDa nonglycosylated peptide. Experiments with whole pollen cultured in vitro show challenge with stigmatic extracts to stimulate the release of gametophytic and sporophytic polypeptides and to result in the formation of a conspicuous interaction product, demonstrating the 7-kDa peptide to be freely available within the coating of pollen in vivo.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • McClure BA, Haring V, Ebert PR, Anderson MA, Simpson RJ, Sakiyama F, Clarke AE. Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature. 1989 Dec 21;342(6252):955–957. [PubMed]
  • Singh A, Ai Y, Kao TH. Characterization of Ribonuclease Activity of Three S-Allele-Associated Proteins of Petunia inflata. Plant Physiol. 1991 May;96(1):61–68. [PMC free article] [PubMed]
  • East EM, Mangelsdorf AJ. A New Interpretation of the Hereditary Behavior of Self-Sterile Plants. Proc Natl Acad Sci U S A. 1925 Feb;11(2):166–171. [PMC free article] [PubMed]
  • Scutt CP, Gates PJ, Gatehouse JA, Boulter D, Croy RR. A cDNA encoding an S-locus specific glycoprotein from Brassica oleracea plants containing the S5 self-incompatibility allele. Mol Gen Genet. 1990 Feb;220(3):409–413. [PubMed]
  • Lalonde BA, Nasrallah ME, Dwyer KG, Chen CH, Barlow B, Nasrallah JB. A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene. Plant Cell. 1989 Feb;1(2):249–258. [PMC free article] [PubMed]
  • Thorsness MK, Kandasamy MK, Nasrallah ME, Nasrallah JB. A Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana. Dev Biol. 1991 Jan;143(1):173–184. [PubMed]
  • Sato T, Thorsness MK, Kandasamy MK, Nishio T, Hirai M, Nasrallah JB, Nasrallah ME. Activity of an S Locus Gene Promoter in Pistils and Anthers of Transgenic Brassica. Plant Cell. 1991 Sep;3(9):867–876. [PMC free article] [PubMed]
  • Toriyama K, Thorsness MK, Nasrallah JB, Nasrallah ME. A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis. Dev Biol. 1991 Feb;143(2):427–431. [PubMed]
  • Elleman CJ, Dickinson HG. Pollen-stigma interactions in Brassica. IV. Structural reorganization in the pollen grains during hydration. J Cell Sci. 1986 Feb;80:141–157. [PubMed]
  • Sarker RH, Elleman CJ, Dickinson HG. Control of pollen hydration in Brassica requires continued protein synthesis, and glycosylation in necessary for intraspecific incompatibility. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4340–4344. [PMC free article] [PubMed]
  • Heslop-Harrison J, Heslop-Harrison Y. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain Technol. 1970 May;45(3):115–120. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Schägger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368–379. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Faye L, Chrispeels MJ. Characterization of N-linked oligosaccharides by affinoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases. Anal Biochem. 1985 Aug 15;149(1):218–224. [PubMed]
  • Fraker PJ, Speck JC., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. [PubMed]
  • Stead AD, Roberts IN, Dickinson HG. Pollen-stigma interaction in Brassica oleracea: the role of stigmatic proteins in pollen grain adhesion. J Cell Sci. 1980 Apr;42:417–423. [PubMed]
  • Stein JC, Howlett B, Boyes DC, Nasrallah ME, Nasrallah JB. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816–8820. [PMC free article] [PubMed]
  • Walker JC, Zhang R. Relationship of a putative receptor protein kinase from maize to the S-locus glycoproteins of Brassica. Nature. 1990 Jun 21;345(6277):743–746. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...