• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Dec 6, 1994; 91(25): 12258–12262.
PMCID: PMC45416

Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage.

Abstract

In an effort to study the molecular basis of the determination processes of the mammalian germ cell lineage, we have tried to isolate a mouse gene homolog to vasa, which plays an essential role as a maternal determining factor for the formation of Drosophila germ cell precursors. By reverse transcriptase PCRs of mouse primordial germ cell cDNAs using family-specific primers, we obtained a gene (Mvh) encoding a DEAD-family protein that showed a much higher degree of similarity with the product of the Drosophila vasa gene (vas) than previously reported mouse genes. In adult tissues, Mvh transcripts were exclusively detected in testicular germ cells, in which Mvh protein was found to be localized in cytoplasm of spermatocytes and round spermatids including a perinuclear granule. The protein was also expressed in germ cells colonized in embryonic gonads but was not detected in pluripotential embryonic cells such as stem cells and germ cells. These results suggest the possibility that the Mvh protein may play an important role in the determination events of mouse germ cells as in the case of Drosophila vasa.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ginsburg M, Snow MH, McLaren A. Primordial germ cells in the mouse embryo during gastrulation. Development. 1990 Oct;110(2):521–528. [PubMed]
  • Mahowald AP. Germ plasm revisited and illuminated. Science. 1992 Mar 6;255(5049):1216–1217. [PubMed]
  • Strome S. Developmental biology. The germ of the issue. Nature. 1992 Jul 30;358(6385):368–369. [PubMed]
  • Lasko PF, Ashburner M. The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A. Nature. 1988 Oct 13;335(6191):611–617. [PubMed]
  • Hay B, Jan LY, Jan YN. A protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases. Cell. 1988 Nov 18;55(4):577–587. [PubMed]
  • Linder P, Lasko PF, Ashburner M, Leroy P, Nielsen PJ, Nishi K, Schnier J, Slonimski PP. Birth of the D-E-A-D box. Nature. 1989 Jan 12;337(6203):121–122. [PubMed]
  • Hay B, Jan LY, Jan YN. Localization of vasa, a component of Drosophila polar granules, in maternal-effect mutants that alter embryonic anteroposterior polarity. Development. 1990 Jun;109(2):425–433. [PubMed]
  • Liang L, Diehl-Jones W, Lasko P. Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities. Development. 1994 May;120(5):1201–1211. [PubMed]
  • Ephrussi A, Lehmann R. Induction of germ cell formation by oskar. Nature. 1992 Jul 30;358(6385):387–392. [PubMed]
  • Hirling H, Scheffner M, Restle T, Stahl H. RNA helicase activity associated with the human p68 protein. Nature. 1989 Jun 15;339(6225):562–564. [PubMed]
  • Lemaire L, Heinlein UA. High-level expression in male germ cells of murine P68 RNA helicase mRNA. Life Sci. 1993;52(11):917–926. [PubMed]
  • Leroy P, Alzari P, Sassoon D, Wolgemuth D, Fellous M. The protein encoded by a murine male germ cell-specific transcript is a putative ATP-dependent RNA helicase. Cell. 1989 May 19;57(4):549–559. [PubMed]
  • Nielsen PJ, Trachsel H. The mouse protein synthesis initiation factor 4A gene family includes two related functional genes which are differentially expressed. EMBO J. 1988 Jul;7(7):2097–2105. [PMC free article] [PubMed]
  • Abe K. Rapid isolation of desired sequences from lone linker PCR amplified cDNA mixtures: application to identification and recovery of expressed sequences in cloned genomic DNA. Mamm Genome. 1992;2(4):252–259. [PubMed]
  • Noce T, Fujiwara Y, Sezaki M, Fujimoto H, Higashinakagawa T. Expression of a mouse zinc finger protein gene in both spermatocytes and oocytes during meiosis. Dev Biol. 1992 Oct;153(2):356–367. [PubMed]
  • Matsui Y, Zsebo K, Hogan BL. Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture. Cell. 1992 Sep 4;70(5):841–847. [PubMed]
  • Komiya T, Itoh K, Ikenishi K, Furusawa M. Isolation and characterization of a novel gene of the DEAD box protein family which is specifically expressed in germ cells of Xenopus laevis. Dev Biol. 1994 Apr;162(2):354–363. [PubMed]
  • de Valoir T, Tucker MA, Belikoff EJ, Camp LA, Bolduc C, Beckingham K. A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2113–2117. [PMC free article] [PubMed]
  • Patterson LF, Harvey M, Lasko PF. Dbp73D, a Drosophila gene expressed in ovary, encodes a novel D-E-A-D box protein. Nucleic Acids Res. 1992 Jun 25;20(12):3063–3067. [PMC free article] [PubMed]
  • Fawcett DW, Eddy EM, Phillips DM. Observations on the fine structure and relationships of the chromatoid body in mammalian spermatogenesis. Biol Reprod. 1970 Feb;2(1):129–153. [PubMed]
  • Parvinen M, Parvinen LM. Active movements of the chromatoid body. A possible transport mechanism for haploid gene products. J Cell Biol. 1979 Mar;80(3):621–628. [PMC free article] [PubMed]
  • Stevens LC. Origin of testicular teratomas from primordial germ cells in mice. J Natl Cancer Inst. 1967 Apr;38(4):549–552. [PubMed]
  • Solter D, Knowles BB. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565–5569. [PMC free article] [PubMed]
  • Schöler HR, Dressler GR, Balling R, Rohdewohld H, Gruss P. Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J. 1990 Jul;9(7):2185–2195. [PMC free article] [PubMed]
  • Noce T, Fujiwara Y, Ito M, Takeuchi T, Hashimoto N, Yamanouchi M, Higashinakagawa T, Fujimoto H. A novel murine zinc finger gene mapped within the tw18 deletion region expresses in germ cells and embryonic nervous system. Dev Biol. 1993 Feb;155(2):409–422. [PubMed]
  • Eddy EM. Germ plasm and the differentiation of the germ cell line. Int Rev Cytol. 1975;43:229–280. [PubMed]
  • Beams HW, Kessel RG. The problem of germ cell determinants. Int Rev Cytol. 1974;39:413–479. [PubMed]
  • Spiegelman M, Bennett D. A light- and electron-microscopic study of primordial germ cells in the early mouse embryo. J Embryol Exp Morphol. 1973 Aug;30(1):97–118. [PubMed]
  • Kobayashi S, Amikura R, Okada M. Presence of mitochondrial large ribosomal RNA outside mitochondria in germ plasm of Drosophila melanogaster. Science. 1993 Jun 4;260(5113):1521–1524. [PubMed]
  • Geremia R, d'Agostino A, Monesi V. Biochemical evidence of haploid gene activity in spermatogenesis of the mouse. Exp Cell Res. 1978 Jan;111(1):23–30. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links