• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Nov 1991; 10(11): 3373–3377.
PMCID: PMC453065

Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response.

Abstract

Glucose repression is a global regulatory mechanism in yeast. We have investigated how glucose regulates the GAL genes, which are required for galactose fermentation. We found that the GAL genes are controlled by a transcriptional cascade. Thus, GAL4, which encodes an activator of the GAL genes, is repressed by MIG1, a zinc finger protein that binds to the GAL4 promoter. MIG1 has a dual role in control of the GAL genes, since MIG1 also binds to the promoter of GAL1, a gene regulated by GAL4. A disruption of MIG1 interacts synergistically with a disruption of GAL80, a gene involved in galactose induction. This suggests that the MIG1-dependent response to glucose is amplified by down-regulation of the induction pathway.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bajwa W, Torchia TE, Hopper JE. Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases. Mol Cell Biol. 1988 Aug;8(8):3439–3447. [PMC free article] [PubMed]
  • Carlson M. Regulation of sugar utilization in Saccharomyces species. J Bacteriol. 1987 Nov;169(11):4873–4877. [PMC free article] [PubMed]
  • Citron BA, Donelson JE. Sequence of the Saccharomyces GAL region and its transcription in vivo. J Bacteriol. 1984 Apr;158(1):269–278. [PMC free article] [PubMed]
  • DOUGLAS HC, HAWTHORNE DC. ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. Genetics. 1964 May;49:837–844. [PMC free article] [PubMed]
  • Entian KD. Glucose repression: a complex regulatory system in yeast. Microbiol Sci. 1986 Dec;3(12):366–371. [PubMed]
  • Finley RL, Jr, Chen S, Ma J, Byrne P, West RW., Jr Opposing regulatory functions of positive and negative elements in UASG control transcription of the yeast GAL genes. Mol Cell Biol. 1990 Nov;10(11):5663–5670. [PMC free article] [PubMed]
  • Flick JS, Johnston M. Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae. Mol Cell Biol. 1990 Sep;10(9):4757–4769. [PMC free article] [PubMed]
  • Giniger E, Ptashne M. Cooperative DNA binding of the yeast transcriptional activator GAL4. Proc Natl Acad Sci U S A. 1988 Jan;85(2):382–386. [PMC free article] [PubMed]
  • Guarente L, Yocum RR, Gifford P. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410–7414. [PMC free article] [PubMed]
  • Johnston M. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiol Rev. 1987 Dec;51(4):458–476. [PMC free article] [PubMed]
  • Johnston M, Davis RW. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Aug;4(8):1440–1448. [PMC free article] [PubMed]
  • Johnston SA, Hopper JE. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6971–6975. [PMC free article] [PubMed]
  • Laughon A, Gesteland RF. Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6827–6831. [PMC free article] [PubMed]
  • Laughon A, Gesteland RF. Primary structure of the Saccharomyces cerevisiae GAL4 gene. Mol Cell Biol. 1984 Feb;4(2):260–267. [PMC free article] [PubMed]
  • Matern H, Holzer H. Catabolite inactivation of the galactose uptake system in yeast. J Biol Chem. 1977 Sep 25;252(18):6399–6402. [PubMed]
  • Matsumoto K, Toh-e A, Oshima Y. Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae. Mol Cell Biol. 1981 Feb;1(2):83–93. [PMC free article] [PubMed]
  • Matsumoto K, Yoshimatsu T, Oshima Y. Recessive mutations conferring resistance to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae. J Bacteriol. 1983 Mar;153(3):1405–1414. [PMC free article] [PubMed]
  • Mylin LM, Johnston M, Hopper JE. Phosphorylated forms of GAL4 are correlated with ability to activate transcription. Mol Cell Biol. 1990 Sep;10(9):4623–4629. [PMC free article] [PubMed]
  • Nehlin JO, Ronne H. Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. EMBO J. 1990 Sep;9(9):2891–2898. [PMC free article] [PubMed]
  • Nehlin JO, Carlberg M, Ronne H. Yeast galactose permease is related to yeast and mammalian glucose transporters. Gene. 1989 Dec 28;85(2):313–319. [PubMed]
  • Nogi Y, Fukasawa T. Nucleotide sequence of the yeast regulatory gene GAL80. Nucleic Acids Res. 1984 Dec 21;12(24):9287–9298. [PMC free article] [PubMed]
  • Ptashne M. How eukaryotic transcriptional activators work. Nature. 1988 Oct 20;335(6192):683–689. [PubMed]
  • Thomas BJ, Rothstein R. Elevated recombination rates in transcriptionally active DNA. Cell. 1989 Feb 24;56(4):619–630. [PubMed]
  • Torchia TE, Hamilton RW, Cano CL, Hopper JE. Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes. Mol Cell Biol. 1984 Aug;4(8):1521–1527. [PMC free article] [PubMed]
  • Tschopp JF, Emr SD, Field C, Schekman R. GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J Bacteriol. 1986 Apr;166(1):313–318. [PMC free article] [PubMed]
  • Yocum RR, Hanley S, West R, Jr, Ptashne M. Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Oct;4(10):1985–1998. [PMC free article] [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...