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EMBO J. Aug 1991; 10(8): 2055–2061.
PMCID: PMC452888

Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion.


The role of the Campylobacter jejuni flagella in adhesion to, and penetration into, eukaryotic cells was investigated. We used homologous recombination to inactivate the two flagellin genes flaA and flaB of C. jejuni, respectively. Mutants in which flaB but not flaA is inactivated remain motile. In contrast a defective flaA gene leads to immotile bacteria. Invasion studies showed that mutants without motile flagella have lost their potential to adhere to, and penetrate into, human intestinal cells in vitro. Invasive properties could be partially restored by centrifugation of the mutants onto the tissue culture cells, indicating that motility is a major, but not the only, factor involved in invasion.

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Selected References

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  • Aguero-Rosenfeld ME, Yang XH, Nachamkin I. Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni. Infect Immun. 1990 Jul;58(7):2214–2219. [PMC free article] [PubMed]
  • Attridge SR, Rowley D. The role of the flagellum in the adherence of Vibrio cholerae. J Infect Dis. 1983 May;147(5):864–872. [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Caldwell MB, Guerry P, Lee EC, Burans JP, Walker RI. Reversible expression of flagella in Campylobacter jejuni. Infect Immun. 1985 Dec;50(3):941–943. [PMC free article] [PubMed]
  • De Melo MA, Gabbiani G, Pechère JC. Cellular events and intracellular survival of Campylobacter jejuni during infection of HEp-2 cells. Infect Immun. 1989 Jul;57(7):2214–2222. [PMC free article] [PubMed]
  • de Melo MA, Pechère JC. Identification of Campylobacter jejuni surface proteins that bind to Eucaryotic cells in vitro. Infect Immun. 1990 Jun;58(6):1749–1756. [PMC free article] [PubMed]
  • Drake D, Montie TC. Flagella, motility and invasive virulence of Pseudomonas aeruginosa. J Gen Microbiol. 1988 Jan;134(1):43–52. [PubMed]
  • Fauchère JL, Kervella M, Rosenau A, Mohanna K, Véron M. Adhesion to HeLa cells of Campylobacter jejuni and C. coli outer membrane components. Res Microbiol. 1989 Jul-Aug;140(6):379–392. [PubMed]
  • Freter R, Jones GW. Adhesive properties of Vibrio cholerae: nature of the interaction with intact mucosal surfaces. Infect Immun. 1976 Jul;14(1):246–256. [PMC free article] [PubMed]
  • Guentzel MN, Berry LJ. Motility as a virulence factor for Vibrio cholerae. Infect Immun. 1975 May;11(5):890–897. [PMC free article] [PubMed]
  • Guerry P, Logan SM, Thornton S, Trust TJ. Genomic organization and expression of Campylobacter flagellin genes. J Bacteriol. 1990 Apr;172(4):1853–1860. [PMC free article] [PubMed]
  • Konkel ME, Joens LA. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. Infect Immun. 1989 Oct;57(10):2984–2990. [PMC free article] [PubMed]
  • Konkel ME, Joens LA. Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates. Infect Immun. 1990 Apr;58(4):1101–1105. [PMC free article] [PubMed]
  • Labigne-Roussel A, Harel J, Tompkins L. Gene transfer from Escherichia coli to Campylobacter species: development of shuttle vectors for genetic analysis of Campylobacter jejuni. J Bacteriol. 1987 Nov;169(11):5320–5323. [PMC free article] [PubMed]
  • Labigne-Roussel A, Courcoux P, Tompkins L. Gene disruption and replacement as a feasible approach for mutagenesis of Campylobacter jejuni. J Bacteriol. 1988 Apr;170(4):1704–1708. [PMC free article] [PubMed]
  • Logan SM, Trust TJ, Guerry P. Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J Bacteriol. 1989 Jun;171(6):3031–3038. [PMC free article] [PubMed]
  • Manninen KI, Prescott JF, Dohoo IR. Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infect Immun. 1982 Oct;38(1):46–52. [PMC free article] [PubMed]
  • McSweegan E, Walker RI. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun. 1986 Jul;53(1):141–148. [PMC free article] [PubMed]
  • Miller JF, Dower WJ, Tompkins LS. High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc Natl Acad Sci U S A. 1988 Feb;85(3):856–860. [PMC free article] [PubMed]
  • Morooka T, Umeda A, Amako K. Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol. 1985 Aug;131(8):1973–1980. [PubMed]
  • Newell DG. Monoclonal antibodies directed against the flagella of Campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice. J Hyg (Lond) 1986 Apr;96(2):131–141. [PMC free article] [PubMed]
  • Newell DG, Pearson A. The invasion of epithelial cell lines and the intestinal epithelium of infant mice by Campylobacter jejuni/coli. J Diarrhoeal Dis Res. 1984 Mar;2(1):19–26. [PubMed]
  • Nuijten PJ, Bleumink-Pluym NM, Gaastra W, van der Zeijst BA. Flagellin expression in Campylobacter jejuni is regulated at the transcriptional level. Infect Immun. 1989 Apr;57(4):1084–1088. [PMC free article] [PubMed]
  • Nuijten PJ, van Asten FJ, Gaastra W, van der Zeijst BA. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem. 1990 Oct 15;265(29):17798–17804. [PubMed]
  • Nuijten PJ, van der Zeijst BA, Newell DG. Localization of immunogenic regions on the flagellin proteins of Campylobacter jejuni 81116. Infect Immun. 1991 Mar;59(3):1100–1105. [PMC free article] [PubMed]
  • Rottier PJ, Spaan WJ, Horzinek MC, van der Zeijst BA. Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes. J Virol. 1981 Apr;38(1):20–26. [PMC free article] [PubMed]
  • Seifert HS, Ajioka RS, Marchal C, Sparling PF, So M. DNA transformation leads to pilin antigenic variation in Neisseria gonorrhoeae. Nature. 1988 Nov 24;336(6197):392–395. [PubMed]
  • Skirrow MB. Campylobacter. Lancet. 1990 Oct 13;336(8720):921–923. [PubMed]
  • Snyder JD, Merson MH. The magnitude of the global problem of acute diarrhoeal disease: a review of active surveillance data. Bull World Health Organ. 1982;60(4):605–613. [PMC free article] [PubMed]
  • Wang Y, Taylor DE. Natural transformation in Campylobacter species. J Bacteriol. 1990 Feb;172(2):949–955. [PMC free article] [PubMed]

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