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EMBO J. Oct 15, 1996; 15(20): 5679–5689.
PMCID: PMC452312

Activation of the CaMV as-1 cis-element by salicylic acid: differential DNA-binding of a factor related to TGA1a.


Transcription from the as-1 element of the cauliflower mosaic virus is induced by salicylic acid (SA), an endogenous signal involved in plant defence responses. Electrophoretic mobility shift assays demonstrated that the binding of a tobacco cellular factor, named SARP, correlates with the SA-induced activation of transcription. SARP was shown to contain proteins immunologically related to TGA1a, a transcription factor previously cloned for its ability to bind to the as-1 element. The molecular mass of SARP was estimated to be 40 kDa by South-Western experiments. Treatment of the extracts with dissociating agents led to an increase in the DNA binding activity, suggesting the presence of an inhibitor that sequesters SARP. The DNA binding activity appeared sensitive to phosphatase treatment, suggesting a role for phosphorylation in the SA-induced gene activation. These results represent an analysis of immediate early response to SA and potentially elucidate the events of the SA signal transduction pathway.

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  • Abel S, Theologis A. Early genes and auxin action. Plant Physiol. 1996 May;111(1):9–17. [PMC free article] [PubMed]
  • Auwerx J, Sassone-Corsi P. IP-1: a dominant inhibitor of Fos/Jun whose activity is modulated by phosphorylation. Cell. 1991 Mar 8;64(5):983–993. [PubMed]
  • Baeuerle PA, Baltimore D. I kappa B: a specific inhibitor of the NF-kappa B transcription factor. Science. 1988 Oct 28;242(4878):540–546. [PubMed]
  • Baeuerle PA, Baltimore D. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor. Cell. 1988 Apr 22;53(2):211–217. [PubMed]
  • Benfey PN, Ren L, Chua NH. The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J. 1989 Aug;8(8):2195–2202. [PMC free article] [PubMed]
  • Benfey PN, Ren L, Chua NH. Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J. 1990 Jun;9(6):1677–1684. [PMC free article] [PubMed]
  • Bi YM, Kenton P, Mur L, Darby R, Draper J. Hydrogen peroxide does not function downstream of salicylic acid in the induction of PR protein expression. Plant J. 1995 Aug;8(2):235–245. [PubMed]
  • Boot KJM, Van Der Zaal BJ, Velterop J, Quint A, Mennes AM, Hooykaas PJJ, Libbenga KR. Further Characterization of Expression of Auxin-Induced Genes in Tobacco (Nicotiana tabacum) Cell-Suspension Cultures. Plant Physiol. 1993 Jun;102(2):513–520. [PMC free article] [PubMed]
  • Cao H, Bowling SA, Gordon AS, Dong X. Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance. Plant Cell. 1994 Nov;6(11):1583–1592. [PMC free article] [PubMed]
  • Chen Z, Klessig DF. Identification of a soluble salicylic acid-binding protein that may function in signal transduction in the plant disease-resistance response. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8179–8183. [PMC free article] [PubMed]
  • Chen Z, Ricigliano JW, Klessig DF. Purification and characterization of a soluble salicylic acid-binding protein from tobacco. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9533–9537. [PMC free article] [PubMed]
  • Chen Z, Silva H, Klessig DF. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science. 1993 Dec 17;262(5141):1883–1886. [PubMed]
  • Daniel V. Glutathione S-transferases: gene structure and regulation of expression. Crit Rev Biochem Mol Biol. 1993;28(3):173–207. [PubMed]
  • Delaney TP, Uknes S, Vernooij B, Friedrich L, Weymann K, Negrotto D, Gaffney T, Gut-Rella M, Kessmann H, Ward E, Ryals J. A central role of salicylic Acid in plant disease resistance. Science. 1994 Nov 18;266(5188):1247–1250. [PubMed]
  • Delaney TP, Friedrich L, Ryals JA. Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6602–6606. [PMC free article] [PubMed]
  • Dietrich RA, Delaney TP, Uknes SJ, Ward ER, Ryals JA, Dangl JL. Arabidopsis mutants simulating disease resistance response. Cell. 1994 May 20;77(4):565–577. [PubMed]
  • Ellis JG, Tokuhisa JG, Llewellyn DJ, Bouchez D, Singh K, Dennis ES, Peacock WJ. Does the ocs-element occur as a functional component of the promoters of plant genes? Plant J. 1993 Sep;4(3):433–443. [PubMed]
  • Fan CM, Maniatis T. Generation of p50 subunit of NF-kappa B by processing of p105 through an ATP-dependent pathway. Nature. 1991 Dec 5;354(6352):395–398. [PubMed]
  • Foulkes NS, Borrelli E, Sassone-Corsi P. CREM gene: use of alternative DNA-binding domains generates multiple antagonists of cAMP-induced transcription. Cell. 1991 Feb 22;64(4):739–749. [PubMed]
  • Fromm H, Katagiri F, Chua NH. An octopine synthase enhancer element directs tissue-specific expression and binds ASF-1, a factor from tobacco nuclear extracts. Plant Cell. 1989 Oct;1(10):977–984. [PMC free article] [PubMed]
  • Fromm H, Katagiri F, Chua NH. The tobacco transcription activator TGA1a binds to a sequence in the 5' upstream region of a gene encoding a TGA1a-related protein. Mol Gen Genet. 1991 Oct;229(2):181–188. [PubMed]
  • Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, Uknes S, Ward E, Kessmann H, Ryals J. Requirement of salicylic Acid for the induction of systemic acquired resistance. Science. 1993 Aug 6;261(5122):754–756. [PubMed]
  • Ghosh S, Baltimore D. Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B. Nature. 1990 Apr 12;344(6267):678–682. [PubMed]
  • Goldsbrough AP, Albrecht H, Stratford R. Salicylic acid-inducible binding of a tobacco nuclear protein to a 10 bp sequence which is highly conserved amongst stress-inducible genes. Plant J. 1993 Apr;3(4):563–571. [PubMed]
  • Green PJ, Kay SA, Chua NH. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J. 1987 Sep;6(9):2543–2549. [PMC free article] [PubMed]
  • Greenberg JT, Guo A, Klessig DF, Ausubel FM. Programmed cell death in plants: a pathogen-triggered response activated coordinately with multiple defense functions. Cell. 1994 May 20;77(4):551–563. [PubMed]
  • Hai T, Curran T. Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity. Proc Natl Acad Sci U S A. 1991 May 1;88(9):3720–3724. [PMC free article] [PubMed]
  • Hiratsuka K, Wu X, Fukuzawa H, Chua NH. Molecular dissection of GT-1 from Arabidopsis. Plant Cell. 1994 Dec;6(12):1805–1813. [PMC free article] [PubMed]
  • Hunter T, Karin M. The regulation of transcription by phosphorylation. Cell. 1992 Aug 7;70(3):375–387. [PubMed]
  • Katagiri F, Chua NH. Plant transcription factors: present knowledge and future challenges. Trends Genet. 1992 Jan;8(1):22–27. [PubMed]
  • Katagiri F, Lam E, Chua NH. Two tobacco DNA-binding proteins with homology to the nuclear factor CREB. Nature. 1989 Aug 31;340(6236):727–730. [PubMed]
  • Katagiri F, Yamazaki K, Horikoshi M, Roeder RG, Chua NH. A plant DNA-binding protein increases the number of active preinitiation complexes in a human in vitro transcription system. Genes Dev. 1990 Nov;4(11):1899–1909. [PubMed]
  • Katagiri F, Seipel K, Chua NH. Identification of a novel dimer stabilization region in a plant bZIP transcription activator. Mol Cell Biol. 1992 Nov;12(11):4809–4816. [PMC free article] [PubMed]
  • Kawata T, Imada T, Shiraishi H, Okada K, Shimura Y, Iwabuchi M. A cDNA clone encoding HBP-1b homologue in Arabidopsis thaliana. Nucleic Acids Res. 1992 Mar 11;20(5):1141–1141. [PMC free article] [PubMed]
  • Kim SR, Kim Y, An G. Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter. Plant Physiol. 1993 Sep;103(1):97–103. [PMC free article] [PubMed]
  • Lam E, Benfey PN, Gilmartin PM, Fang RX, Chua NH. Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7890–7894. [PMC free article] [PubMed]
  • Lam E, Katagiri F, Chua NH. Plant nuclear factor ASF-1 binds to an essential region of the nopaline synthase promoter. J Biol Chem. 1990 Jun 15;265(17):9909–9913. [PubMed]
  • Levine A, Tenhaken R, Dixon R, Lamb C. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell. 1994 Nov 18;79(4):583–593. [PubMed]
  • Liu X, Lam E. Two binding sites for the plant transcription factor ASF-1 can respond to auxin treatments in transgenic tobacco. J Biol Chem. 1994 Jan 7;269(1):668–675. [PubMed]
  • Malamy J, Carr JP, Klessig DF, Raskin I. Salicylic Acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science. 1990 Nov 16;250(4983):1002–1004. [PubMed]
  • Miao ZH, Lam E. Construction of a trans-dominant inhibitor for members of the TGA family of transcription factors conserved in higher plants. Plant J. 1995 Jun;7(6):887–896. [PubMed]
  • Miao ZH, Liu X, Lam E. TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana. Plant Mol Biol. 1994 Apr;25(1):1–11. [PubMed]
  • Miskimins WK, Roberts MP, McClelland A, Ruddle FH. Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6741–6744. [PMC free article] [PubMed]
  • Neuhaus G, Neuhaus-Url G, Katagiri F, Seipel K, Chua NH. Tissue-Specific Expression of as-1 in Transgenic Tobacco. Plant Cell. 1994 Jun;6(6):827–834. [PMC free article] [PubMed]
  • Paine PL, Austerberry CF, Desjarlais LJ, Horowitz SB. Protein loss during nuclear isolation. J Cell Biol. 1983 Oct;97(4):1240–1242. [PMC free article] [PubMed]
  • Papavassiliou AG, Bohmann K, Bohmann D. Determining the effect of inducible protein phosphorylation on the DNA-binding activity of transcription factors. Anal Biochem. 1992 Jun;203(2):302–309. [PubMed]
  • Picard D, Salser SJ, Yamamoto KR. A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptor. Cell. 1988 Sep 23;54(7):1073–1080. [PubMed]
  • Qin XF, Holuigue L, Horvath DM, Chua NH. Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-1 element. Plant Cell. 1994 Jun;6(6):863–874. [PMC free article] [PubMed]
  • Rieping M, Fritz M, Prat S, Gatz C. A dominant negative mutant of PG13 suppresses transcription from a cauliflower mosaic virus 35S truncated promoter in transgenic tobacco plants. Plant Cell. 1994 Aug;6(8):1087–1098. [PMC free article] [PubMed]
  • Ryals J, Uknes S, Ward E. Systemic Acquired Resistance. Plant Physiol. 1994 Apr;104(4):1109–1112. [PMC free article] [PubMed]
  • Schindler U, Beckmann H, Cashmore AR. TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG. Plant Cell. 1992 Oct;4(10):1309–1319. [PMC free article] [PubMed]
  • Shulaev V, Leon J, Raskin I. Is Salicylic Acid a Translocated Signal of Systemic Acquired Resistance in Tobacco? Plant Cell. 1995 Oct;7(10):1691–1701. [PMC free article] [PubMed]
  • Tabata T, Nakayama T, Mikami K, Iwabuchi M. HBP-1a and HBP-1b: leucine zipper-type transcription factors of wheat. EMBO J. 1991 Jun;10(6):1459–1467. [PMC free article] [PubMed]
  • Uknes S, Dincher S, Friedrich L, Negrotto D, Williams S, Thompson-Taylor H, Potter S, Ward E, Ryals J. Regulation of pathogenesis-related protein-1a gene expression in tobacco. Plant Cell. 1993 Feb;5(2):159–169. [PMC free article] [PubMed]
  • Ulmasov T, Hagen G, Guilfoyle T. The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues. Plant Mol Biol. 1994 Nov;26(4):1055–1064. [PubMed]
  • Vernooij B, Uknes S, Ward E, Ryals J. Salicylic acid as a signal molecule in plant-pathogen interactions. Curr Opin Cell Biol. 1994 Apr;6(2):275–279. [PubMed]
  • Vernooij B, Friedrich L, Morse A, Reist R, Kolditz-Jawhar R, Ward E, Uknes S, Kessmann H, Ryals J. Salicylic Acid Is Not the Translocated Signal Responsible for Inducing Systemic Acquired Resistance but Is Required in Signal Transduction. Plant Cell. 1994 Jul;6(7):959–965. [PMC free article] [PubMed]
  • Vinson CR, Hai T, Boyd SM. Dimerization specificity of the leucine zipper-containing bZIP motif on DNA binding: prediction and rational design. Genes Dev. 1993 Jun;7(6):1047–1058. [PubMed]
  • Ward ER, Uknes SJ, Williams SC, Dincher SS, Wiederhold DL, Alexander DC, Ahl-Goy P, Metraux JP, Ryals JA. Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance. Plant Cell. 1991 Oct;3(10):1085–1094. [PMC free article] [PubMed]
  • Zhang B, Singh KB. ocs element promoter sequences are activated by auxin and salicylic acid in Arabidopsis. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2507–2511. [PMC free article] [PubMed]
  • Zhang B, Foley RC, Singh KB. Isolation and characterization of two related Arabidopsis ocs-element bZIP binding proteins. Plant J. 1993 Oct;4(4):711–716. [PubMed]
  • Ziff EB. Transcription factors: a new family gathers at the cAMP response site. Trends Genet. 1990 Mar;6(3):69–72. [PubMed]

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