• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Nov 8, 1994; 91(23): 11212–11216.
PMCID: PMC45197

Identification of genes involved in DNA replication of the Autographa californica baculovirus.

Abstract

By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-1, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the AcMNPV pcna gene, encoding a protein with sequence homology to proliferating-cell nuclear antigen, was observed. A pattern of amino acids found in a number of single-stranded-DNA-binding proteins was identified in the carboxyl-terminal region of IE-1.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Blissard GW, Rohrmann GF. Baculovirus diversity and molecular biology. Annu Rev Entomol. 1990;35:127–155. [PubMed]
  • Kool M, Vlak JM. The structural and functional organization of the Autographa californica nuclear polyhedrosis virus genome. Arch Virol. 1993;130(1-2):1–16. [PubMed]
  • Pearson M, Bjornson R, Pearson G, Rohrmann G. The Autographa californica baculovirus genome: evidence for multiple replication origins. Science. 1992 Sep 4;257(5075):1382–1384. [PubMed]
  • Kool M, van den Berg PM, Tramper J, Goldbach RW, Vlak JM. Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. Virology. 1993 Jan;192(1):94–101. [PubMed]
  • Kool M, Voeten JT, Goldbach RW, Tramper J, Vlak JM. Identification of seven putative origins of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA replication. J Gen Virol. 1993 Dec;74(Pt 12):2661–2668. [PubMed]
  • Leisy DJ, Rohrmann GF. Characterization of the replication of plasmids containing hr sequences in baculovirus-infected Spodoptera frugiperda cells. Virology. 1993 Oct;196(2):722–730. [PubMed]
  • Kool M, Voeten JT, Goldbach RW, Vlak JM. Functional mapping of regions of the Autographa californica nuclear polyhedrosis viral genome required for DNA replication. Virology. 1994 Feb;198(2):680–689. [PubMed]
  • Guarino LA, Gonzalez MA, Summers MD. Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus. J Virol. 1986 Oct;60(1):224–229. [PMC free article] [PubMed]
  • Lu A, Carstens EB. Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. Virology. 1991 Mar;181(1):336–347. [PubMed]
  • Pearson MN, Bjornson RM, Ahrens C, Rohrmann GF. Identification and characterization of a putative origin of DNA replication in the genome of a baculovirus pathogenic for Orgyia pseudotsugata. Virology. 1993 Dec;197(2):715–725. [PubMed]
  • Tomalski MD, Wu JG, Miller LK. The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. Virology. 1988 Dec;167(2):591–600. [PubMed]
  • O'Reilly DR, Crawford AM, Miller LK. Viral proliferating cell nuclear antigen. Nature. 1989 Feb 16;337(6208):606–606. [PubMed]
  • Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 1977 Apr;13(4):213–217. [PubMed]
  • Hink WF. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature. 1970 May 2;226(5244):466–467. [PubMed]
  • Passarelli AL, Miller LK. Identification and characterization of lef-1, a baculovirus gene involved in late and very late gene expression. J Virol. 1993 Jun;67(6):3481–3488. [PMC free article] [PubMed]
  • Possee RD, Sun TP, Howard SC, Ayres MD, Hill-Perkins M, Gearing KL. Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp EcoRI-I and -R (polyhedrin gene) region. Virology. 1991 Nov;185(1):229–241. [PubMed]
  • Passarelli AL, Miller LK. Three baculovirus genes involved in late and very late gene expression: ie-1, ie-n, and lef-2. J Virol. 1993 Apr;67(4):2149–2158. [PMC free article] [PubMed]
  • Li Y, Passarelli AL, Miller LK. Identification, sequence, and transcriptional mapping of lef-3, a baculovirus gene involved in late and very late gene expression. J Virol. 1993 Sep;67(9):5260–5268. [PMC free article] [PubMed]
  • Hoheisel JD. A cassette with seven unique restriction sites, including octanucleotide sequences: extension of multiple-cloning-site plasmids. Gene. 1989 Aug 1;80(1):151–154. [PubMed]
  • Friesen PD, Miller LK. Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1987 Jul;61(7):2264–2272. [PMC free article] [PubMed]
  • Guarino LA, Summers MD. Nucleotide sequence and temporal expression of a baculovirus regulatory gene. J Virol. 1987 Jul;61(7):2091–2099. [PMC free article] [PubMed]
  • Carson DD, Guarino LA, Summers MD. Functional mapping of an AcNPV immediately early gene which augments expression of the IE-1 trans-activated 39K gene. Virology. 1988 Feb;162(2):444–451. [PubMed]
  • Carson DD, Summers MD, Guarino LA. Molecular analysis of a baculovirus regulatory gene. Virology. 1991 May;182(1):279–286. [PubMed]
  • Krappa R, Knebel-Mörsdorf D. Identification of the very early transcribed baculovirus gene PE-38. J Virol. 1991 Feb;65(2):805–812. [PMC free article] [PubMed]
  • Crawford AM, Miller LK. Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1988 Aug;62(8):2773–2781. [PMC free article] [PubMed]
  • Koetsier PA, Schorr J, Doerfler W. A rapid optimized protocol for downward alkaline Southern blotting of DNA. Biotechniques. 1993 Aug;15(2):260–262. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Lu A, Carstens EB. Immediate-early baculovirus genes transactivate the p143 gene promoter of Autographa californica nuclear polyhedrosis virus. Virology. 1993 Aug;195(2):710–718. [PubMed]
  • Clem RJ, Fechheimer M, Miller LK. Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science. 1991 Nov 29;254(5036):1388–1390. [PubMed]
  • Wang YS, Hall JD. Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8). J Virol. 1990 May;64(5):2082–2089. [PMC free article] [PubMed]
  • Telford EA, Watson MS, McBride K, Davison AJ. The DNA sequence of equine herpesvirus-1. Virology. 1992 Jul;189(1):304–316. [PubMed]
  • Chee MS, Bankier AT, Beck S, Bohni R, Brown CM, Cerny R, Horsnell T, Hutchison CA, 3rd, Kouzarides T, Martignetti JA, et al. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. [PubMed]
  • Messerle M, Keil GM, Schneider K, Koszinowski UH. Characterization of the murine cytomegalovirus genes encoding the major DNA binding protein and the ICP18.5 homolog. Virology. 1992 Nov;191(1):355–367. [PubMed]
  • Davison AJ. Channel catfish virus: a new type of herpesvirus. Virology. 1992 Jan;186(1):9–14. [PubMed]
  • Huybrechts R, Guarino L, Van Brussel M, Vulsteke V. Nucleotide sequence of a transactivating Bombyx mori nuclear polyhedrosis virus immediate early gene. Biochim Biophys Acta. 1992 Feb 11;1129(3):328–330. [PubMed]
  • Theilmann DA, Stewart S. Identification and characterization of the IE-1 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology. 1991 Feb;180(2):492–508. [PubMed]
  • Wu CA, Nelson NJ, McGeoch DJ, Challberg MD. Identification of herpes simplex virus type 1 genes required for origin-dependent DNA synthesis. J Virol. 1988 Feb;62(2):435–443. [PMC free article] [PubMed]
  • Fixman ED, Hayward GS, Hayward SD. trans-acting requirements for replication of Epstein-Barr virus ori-Lyt. J Virol. 1992 Aug;66(8):5030–5039. [PMC free article] [PubMed]
  • Pari GS, Kacica MA, Anders DG. Open reading frames UL44, IRS1/TRS1, and UL36-38 are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA synthesis. J Virol. 1993 May;67(5):2575–2582. [PMC free article] [PubMed]
  • Pari GS, Anders DG. Eleven loci encoding trans-acting factors are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA replication. J Virol. 1993 Dec;67(12):6979–6988. [PMC free article] [PubMed]
  • Hershberger PA, Dickson JA, Friesen PD. Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication. J Virol. 1992 Sep;66(9):5525–5533. [PMC free article] [PubMed]
  • Gombart AF, Blissard GW, Rohrmann GF. Characterization of the genetic organization of the HindIII M region of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata reveals major differences among baculoviruses. J Gen Virol. 1989 Jul;70(Pt 7):1815–1828. [PubMed]
  • White E, Stillman B. Expression of adenovirus E1B mutant phenotypes is dependent on the host cell and on synthesis of E1A proteins. J Virol. 1987 Feb;61(2):426–435. [PMC free article] [PubMed]
  • Rice WC, Miller LK. Baculovirus transcription in the presence of inhibitors and in nonpermissive Drosophila cells. Virus Res. 1986 Nov;6(2):155–172. [PubMed]
  • Guarino LA, Summers MD. Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene. J Virol. 1986 Feb;57(2):563–571. [PMC free article] [PubMed]
  • Gao M, Knipe DM. Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression. J Virol. 1991 May;65(5):2666–2675. [PMC free article] [PubMed]
  • Gauss P, Krassa KB, McPheeters DS, Nelson MA, Gold L. Zinc (II) and the single-stranded DNA binding protein of bacteriophage T4. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8515–8519. [PMC free article] [PubMed]
  • Chang LS, Shenk T. The adenovirus DNA-binding protein stimulates the rate of transcription directed by adenovirus and adeno-associated virus promoters. J Virol. 1990 May;64(5):2103–2109. [PMC free article] [PubMed]
  • Haynes LL, Rothman-Denes LB. N4 virion RNA polymerase sites of transcription initiation. Cell. 1985 Jun;41(2):597–605. [PubMed]
  • Challberg MD. A method for identifying the viral genes required for herpesvirus DNA replication. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9094–9098. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...