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J Clin Invest. Jul 15, 2004; 114(2): 250–259.
PMCID: PMC449747

Hepatitis C virus mutation affects proteasomal epitope processing

Abstract

The high incidence of hepatitis C virus (HCV) persistence raises the question of how HCV interferes with host immune responses. Studying a single-source HCV outbreak, we identified an HCV mutation that impaired correct carboxyterminal cleavage of an immunodominant HLA-A2–restricted CD8 cell epitope that is frequently recognized by recovered patients. The mutation, a conservative HCV nonstructural protein 3 (NS3) tyrosine to phenylalanine substitution, was absent in 54 clones of the infectious source, but present in 15/21 (71%) HLA-A2–positive and in 11/24 (46%) HLA-A2–negative patients with chronic hepatitis C. In order to analyze whether the mutation affected the processing of the HLA-A2–restricted CD8 cell epitope, mutant and wild-type NS3 polypeptides were digested in vitro with 20S constitutive proteasomes and with immunoproteasomes. The presence of the mutation resulted in impaired carboxyterminal cleavage of the epitope. In order to analyze whether impaired epitope processing affected T cell priming in vivo, HLA-A2–transgenic mice were infected with vaccinia viruses encoding either wild-type or mutant HCV NS3. The mutant induced fewer epitope-specific, IFN-γ;–producing and fewer tetramer+ cells than the wild type. These data demonstrate how a conservative mutation in the flanking region of an HCV epitope impairs the induction of epitope-specific CD8+ T cells and reveal a mechanism that may contribute to viral sequence evolution in infected patients.

Introduction

Hepatitis C virus (HCV) is a 9.6 kb positive-stranded RNA virus of the flavivirus family and the leading cause of chronic hepatitis worldwide. Whereas recovery from acute HCV infection has been associated with multispecific T cell responses that protect upon reexposure to the virus (1, 2), these responses are either not induced or not maintained in the large number of patients who develop chronic infection (35).

We have therefore asked whether HCV interferes with the induction of antigen-specific T cells. Induction of CD8+ T cells depends on the generation of MHC class I ligands by the proteasome, the major cytosolic proteinase. The proteasome cleaves short peptides from longer polypeptide precursors that are then translocated into the endoplasmic reticulum and bind to newly synthesized MHC class I molecules (6). 26S proteasomes contain a 20S catalytic core, arranged as 2 heptameric outer rings with 7 β-subunits each and 2 heptameric inner rings with 7 α-subunits each. Proteasome activity is closely regulated by cytokines that are produced in viral infections (712). In response to IFN-γ;, for example, the constitutive catalytic subunits β1, β2, and β5 are replaced by low molecular weight protein 2 (LMP2) (iβ1), LMP7 (iβ5), and multicatalytic endopeptidase complex-like–1 (MECL-1) (iβ2) to form immunoproteasomes with altered cleavage properties (13).

HCV circulates in an abundant number of quasispecies because of its high replication rate (14) and its lack of polymerase proofreading capacity. Individual HCV sequences have been described as abolishing recognition by T cell receptors (TCRs) and antibodies, thus interfering with the effector arms of the cellular (2, 15, 16) and humoral immune responses (17). In contrast, the possibility that HCV mutations affect the induction of T cell responses has not been investigated. Indirect evidence for this hypothesis stems from descriptive reports that HCV isolates from persistently infected patients often encode less immunogenic sequences than prototype peptides used for in vitro analysis (18, 19). Whether the decreased immunogenicity results from viral mutations or from infection with less immunogenic strains has not been analyzed because the sequence of the infecting virus is not known in most human studies and also because the route of infection and inoculum size differ among the studied individuals.

Having studied a cohort of patients accidentally infected by a single-source HCV with known sequence, we here demonstrate that an HCV mutation located in the flanking region of a frequently recognized HCV epitope (4, 2025) impairs the induction of HCV-specific CD8+ T cells by affecting the sophisticated proteasomal antigen-processing machinery.

Results

The tyrosine/phenylalanine mutation at residue HCV nonstructural protein 31082 is common in patients who develop persistent infection after a single-source outbreak of HCV.

In order to study viral mutations during the natural course of HCV infection in humans, we analyzed a cohort of patients that had accidentally been infected with HCV in 1978/1979 during a single-source outbreak due to a contaminated anti–D immunoglobulin (26). Because the precise time of infection as well as the genotype and the sequence of the original infectious virus were known and identical for all patients, this cohort was suitable for studying candidate mechanisms of viral persistence. As previously described for this and other cohorts, the strength of the cellular immune response correlated with the outcome of infection, and HCV nonstructural protein 3 (NS3) peptides were among the most frequently recognized (3, 5, 20, 27, 28).

When we compared the HCV NS3987–1133 sequence of the infectious source with sequences that we isolated from the sera of persistently infected, HLA-A2–positive patients 18 years after the single-source outbreak, we found the highest ratio of nonsynonymous to synonymous HCV mutations at amino acid position NS31082 (Figure (Figure1).1). Position NS31082 was located directly adjacent to the carboxyterminus of an HLA-A2–restricted CD8+ T cell epitope recognized by recovered patients of this (20) and other cohorts (4, 2025). Whereas 54 molecular clones from 3 independent PCRs of the infectious source encoded a tyrosine (Y) in position NS31082, serum isolates from 15 of 21 (71%) HLA-A2–positive, persistently infected patients encoded a phenylalanine (F) at position NS31082 (Table (Table1).1). Because the Y/F mutant was also observed in a substantial number of HLA-A2–negative, persistently infected patients (11 of 24 [46%]; P = 0.07; data not shown), the epidemiological data alone did not indicate whether HLA-A2–restricted T cell selection pressure could have contributed to the viral sequence evolution in this patient cohort. We therefore decided to analyze the molecular and immunological effects of the Y/F mutation in vitro by studying proteasomal processing of antigen and in vivo by studying its effect on T cell priming in an HLA-transgenic mouse model.

Figure 1
Prevalence of synonymous (open circles) and nonsynonymous (filled circles) HCV mutations within the HCV NS3987–1133 sequence isolated from HLA-A2 positive, persistently infected HCV patients 18 years after a single-source outbreak of hepatitis ...
Table 1
HCV NS31062–1095 sequence of the infectious source and HLA-A2+ HCV-infected patients

The Y/F mutation at residue N31082 impairs carboxyterminal processing of the NS31073–1081 epitope.

Most MHC class I–restricted peptides are liberated from antigenic precursor sequences by the 20S core particle of the proteasome. To analyze whether the NS31082 Y/F substitution affected proteasomal processing of the NS31073–1081 epitope, we built on our previous demonstration that proteasome-dependent in vitro processing of epitope-harboring polypeptides reflects the in vivo situation with high fidelity (10, 12, 29, 30) and compared proteasome-dependent in vitro processing of the NS3 wild-type polypeptide, designated NS3(Wt)1062–1095, with processing of the NS3 mutant polypeptide, designated NS3(Mut)1062–1095. For this purpose, immunoproteasomes were purified from IFN-γ;–stimulated HepG2 human hepatoma cells and from murine mouse embryonal cells–217 (MEC-217) transfected with the immunoproteasome subunits LMP2, LMP7 and MECL-1 (10). Constitutive proteasomes were purified from unstimulated HepG2 cells and from murine MEC-18 cells.

Synthetic NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptides (Figure (Figure2a)2a) were then incubated with 20S immunoproteasomes. The digestion products were separated and analyzed by reversed phase–HPLC and mass spectrometry. NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptide substrates were digested with the same kinetics with nearly 40–50% turn-over of each substrate within 4–8 hours (Figure (Figure2B).2B). Because longer digestion times resulted in secondary cleavage of processing intermediates, 4-hour and 8-hour digestion times were considered optimal for further biochemical analyses. Processing with constitutive proteasomes yielded the same qualitative results but lower amounts of digest product than processing with immunoproteasomes (not shown).

Figure 2
Digestion of NS3(Wt)1062–1095 polypeptide and NS3(Mut)1062–1095 polypeptide with purified 20S immunoproteasomes. (A) The amino acid sequences of the 34-mer NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptides are shown in ...

In contrast to the substrates, the relative abundance of the NS31073–1081 epitope could not be precisely determined because the epitope’s 2 cysteine residues formed aggregates via disulfide bonds. These aggregates interfered with reliable identification of the NS31073–1081 epitope and its immediate precursors by mass spectrometry. As an indirect measure of the generation of the NS31073–1081 epitope, we therefore determined the relative abundance of those cleavage products that flanked its amino- and carboxyterminus. Cleavage product NS31062–1070, for example, flanked the amino-terminus of the NS31073–1081 epitope. Cleavage product NS31062–1070 was generated from both the NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptide substrates with comparable efficiency (Figure (Figure2C).2C). Generation of cleavage product NS31062–1070 was associated with the generation of the complementary cleavage product NS31071–1095 (Figure (Figure2D),2D), consistent with a proteasomal cut between amino acid positions 1070 and 1071 in both the NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptide substrates (see dotted line in Figure Figure2A).2A). This proteasomal cut resulted in an amino-terminal elongation of the NS31073–1081 epitope by 2 amino acids.

A second cleavage product that flanked the amino-terminus of the NS31073–1081 epitope was peptide NS31062–1072. As indicated in Figure Figure2E,2E, cleavage product NS31062–1072 was also liberated from both the NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptide substrates in comparable amounts (Figure (Figure2E),2E), indicating that the proteasome cut both the NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptide between amino acid positions 1072 and 1073 as indicated by the dotted line in Figure Figure2A.2A. This proteasomal cut resulted in the generation of the correct amino-terminus of the NS31073–1081 epitope, irrespective of the presence or absence of the NS31082 mutation. The demonstration that the NS31073–1081 epitope and its amino-terminally elongated forms were generated at the same time is consistent with previous findings for other epitopes (31, 32). In cases of amino-terminally elongated peptides, the precise amino-terminus has been shown to be further defined by postproteasomal trimming by aminoexopeptidases (31, 32).

In contrast to the amino-terminus, the correct carboxyterminus of the NS31073–1081 epitope was generated only from the wild-type polypeptide. Figure Figure22 (F, G, and H) shows the relative abundance of those cleavage products whose generation defined the epitope’s carboxyterminus at amino acid position NS31082. The generation of cleavage product NS31082–1095 indicated the correct processing of the epitope’s carboxyterminus with a proteasomal cut between amino acid positions 1081 and 1082 (Figure (Figure2F).2F). Importantly, cleavage product NS31082–1095 was only liberated from the NS3(Wt)1062–1095and not from the NS3(Mut)1062–1095 polypeptide substrate (Figure (Figure2F).2F). In contrast, cleavage product NS31083–1095 was liberated from the NS3(Mut)1062–1095 more efficiently than from the NS3(Wt)1062–1095 polypeptide substrate (Figure (Figure2G).2G). This result indicated that, in the presence of the NS31082 mutation, the proteasome cut between amino acid positions 1082 and 1083 rather than between amino acid positions 1081 and 1082 and thus generated a carboxyterminally elongated NS3 epitope. Finally, cleavage product NS31084–1095 was liberated from both the NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptide substrates in comparable amounts (Figure (Figure2H),2H), indicating that the proteasomal cut between amino acid 1083 and 1084 was not affected by the NS31082 mutation.

In summary, an incorrect carboxyterminus of the NS31073–1081 epitope appeared to be generated from the NS3(Mut)1062–1095 but not from the NS3(Wt)1062–1095 polypeptide substrate.

NS31073–1081-specific CD8+ T cells recognize aminoterminally, but not carboxyterminally elongated forms of the minimal optimal epitope.

To study recognition of the processing products by CD8+ T cells, we generated NS31073–1081-specific CD8+ T cell lines from the blood of HCV-recovered patients. These T cell lines recognized target cells pulsed with the minimal optimal NS31073–1081-epitope in a standard cytotoxicity assay (Figure (Figure3).3). Epitope-specific T cell lines also recognized the corresponding aminoterminally elongated peptides, but not the carboxyterminally elongated mutant and wild-type peptides (Figure (Figure3),3), thereby confirming that correct carboxyterminal cleavage by the proteasome was indispensable (33, 34).

Figure 3
NS31073–1081 epitope–specific CD8+ T cells recognize aminoterminally extended, but not carboxyterminally extended, peptides of the minimal optimal NS31073–1081 epitope. NS31073–1081–specific CD8+ ...

Direct biochemical and immunological detection of the NS31073–1081 epitope in proteasomal digests.

To directly assess and quantitate the in vitro generation of the NS31073–1081 epitope, it was necessary to prevent the formation of disulfide bonds between the cysteine residues. We therefore synthesized a serine variant of the epitope with a cysteine to serine exchange at position NS31073. This NS31073–1081 serine variant was equally well recognized by cytotoxic T cells as the original NS31073–1081 epitope (Figure (Figure44A).

Figure 4
Biochemical and immunological analysis of proteasomal digests. (A) NS31073–1081–specific CD8+ T cells lyse T2 cells loaded with either the NS31073–1081 serine variant SVNGVCWTV or the NS31073–1081 epitope CVNGVCWTV. ...

When the corresponding NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptides with a serine in position NS31073 were subjected to digestion by constitutive proteasomes, the production of the NS31073–1081 serine variant could be directly assessed by mass spectrometry. As demonstrated in Figure Figure4B,4B, a significantly larger amount of the NS31073–1081 SVNGVCWTV epitope was generated from the NS3(Wt)1062–1095 polypeptide than from the NS3(Mut)1062–1095 polypeptide. For immunological analysis, the complete 8-hour proteasomal digests were then loaded onto transporter associated with antigen processing–deficient (TAP-deficient), T2-target cells and tested for recognition by NS31073–1081-specific, cytotoxic T cell lines. Only the wild-type and not the mutant polypeptide digests were recognized by NS31073–1081-specific cytotoxic T cells (Figure (Figure4D),4D), thus confirming the biochemical data in Figure Figure22.

The same qualitative results were obtained when NS3(Wt)1062–1095 and NS3(Mut)1062–1095 polypeptides were digested with immunoproteasome instead of constitutive proteasome. Again, a significantly larger amount of the NS31073–1081 SVNGVCWTV epitope was generated from NS3(Wt)1062–1095 than from NS3(Mut)1062–1095 polypeptide (Figure (Figure4,4, C and E). Overall, the immunoproteasome appeared to digest the wild-type polypeptide more rapidly than the constitutive proteasome did, as indicated by a plateau-phase of epitope liberation from the wild-type polypeptide in the biochemical analysis (Figure (Figure4C)4C) and by the higher cytotoxicity in the immunological analysis (Figure (Figure44E).

The HCV NS31082 Y/F mutant reduces the induction of HCV NS31073–1081-specific CD8+ T cells in HLA-A2 transgenic mice.

To analyze whether the altered proteasomal cleavage of the NS31082 Y/F mutant affected the generation of the NS31073–1081 epitope in vivo, we employed a humanized mouse model. Specifically, we used transgenic mice that expressed the α1 and α2 chains of the human HLA-A2 molecule and the α3 chain of the murine Kd molecule (35). Upon immunization, these mice generate T cells against the same HLA-A2–restricted epitopes as HLA-A2–positive humans (36). In contrast to what occurs in human studies, however, this mouse model allows the induction of T cells in the context of a single, defined HLA molecule and a single, defined viral sequence. Therefore, the HLA-A2–transgenic mouse model is not subject to the influences of additional factors such as selection pressure in the context of other HLA molecules and epitopes and/or preservation of viral replication fitness as may be operating in vivo in infected humans.

To take advantage of this model, we immunized HLA-A2–transgenic mice with vaccinia viruses that encoded full-length HCV NS3 sequences with either the Y wild type or the F mutant at amino acid position NS31082. Two weeks after immunization, spleen cells were isolated, and the frequency of NS31073–1081-specific T cells was assessed by ex vivo intracellular IFN-γ;–staining and by tetramer analysis. As shown in Figure Figure5A5A for individual mice and in Figure Figure5,5, B and C, for all mice, the frequency of NS31073–1081-specific, IFN-γ;–producing and tetramer+ cells was significantly higher in mice infected with wild-type than in those infected with mutant NS3 encoding virus. In contrast, the frequency of CD8+ cells that recognized either a vaccinia virus epitope or an unrelated HCV NS3 epitope did not differ between both groups of mice, thus confirming the specificity of the observation.

Figure 5
Immunization of HLA-A2 transgenic mice with the wild-type HCV NS3 sequence induces more IFN-γ;–secreting and cytotoxic HCV NS31073–1081–specific CD8 T cells than immunization with the mutant HCV NS3 sequence. (A and B) ...

In separate experiments, additional immunizations with recombinant DNA-expression vectors were performed to increase the number of NS31073–1081-specific T cells and to establish T cell lines suitable for cytotoxicity analyses. At all effector/target ratios, cytotoxic T cell responses of mice that had been immunized with wild-type NS3 sequences were significantly stronger than cytotoxic T cell responses of mice immunized with mutant NS3 sequences (Figure (Figure5D).5D). Collectively, these results demonstrate that the NS31082 Y/F substitution reduced the generation of the NS31073–1081 epitope in vitro when polypeptides were digested with purified 20S proteasomes. In addition, the NS31082 substitution impaired the induction of epitope-specific T cells in vivo when full-length NS3 protein was endogenously expressed, ubiquitinylated, and processed by the 26S proteasome in the presence of additional cytosolic proteases.

Discussion

Most MHC class I ligands are liberated from strings of polypeptides and ubiquitinylated proteins by the proteasome, the main cytosolic protease (13). Whereas the amino-terminus of each epitope can be further defined by postproteasomal aminoexopeptidases (31, 32, 37), the carboxyterminus needs to be defined precisely with the first cut (33, 34). Studying a single source outbreak of HCV, we identified an HCV mutation that interfered with the correct carboxyterminal cleavage of an immunodominant, HLA-A2 restricted HCV epitope from its mutated polypeptide precursor.

The emergence of viral mutations in immunogenic sequences has long been discussed as a potential immune escape mechanism. As regards HCV, sequences that do not bind to the MHC and/or the T cell receptor and thus are not recognized by HCV-specific CD8+ T cells have been observed in chimpanzees and in humans (15, 16, 18). In addition, mutations that generate partial agonists or antagonists to the T cell receptor and downregulate wild-type–specific T cell responses have been described in HCV (18) as well as in hepatitis B virus (HBV) (38) and HIV infections (39). Perhaps even more efficient mechanisms of viral escape are mutations in epitope-flanking residues that interfere with antigen processing and presentation of MHC class I–restricted epitopes because, in these cases, the induction phase rather than the effector phase of HCV-specific T cell responses can be impaired. Indeed, the literature provides several examples showing that amino acid residues in the flanking regions of T cell epitopes impair proteasomal processing of those epitopes (4043). On the other hand, there are also examples showing that extensive sequence changes in the flanking regions of other immunodominant CD8 T cell epitopes do not influence antigen processing (44). In our study, we observed impairment of antigen processing by an exchange of two very similar amino acids. Although the Y/F substitution is a conservative one, it impaired correct carboxyterminal cleavage of the NS31073–1081 epitope not only by constitutive proteasomes, but also by immunoproteasomes. Importantly, the same effects were observed in vitro when polypeptides were digested with purified 20S proteasomes and in vivo when full length HCV NS3 protein was endogenously expressed in an animal model, ubiquitinylated, and processed by the 26S proteasome in the presence of additional cytosolic proteases. Thus, the data demonstrate a mechanism by which a conservative HCV mutation can interfere with the induction of epitope-specific CD8+ T cells.

The observation that this specific HCV mutation was also found in a substantial number of HLA-A2–negative patients is consistent with a recent report on an HIV mutation in an HLA-B51–restricted epitope, which was also less frequent in HLA-B*51-negative than in HLA-B*51-positive persons, but not completely absent in HLA-B*51-negative persons. Overall, 29% of HLA-B*51-negative persons carried the HIV mutation as compared to 98% of HLA-B*51-positive persons (43). Notably, with more than 400 patients enrolled, the HIV study was much larger than our study, and differences in the prevalence of the HIV mutation between patient subgroups were remarkable and statistically significant (43). In the HIV study as well as in our study, however, the presence of the mutation in a subpopulation of patients without the relevant HLA haplotype is consistent with the influence of multiple selection forces that drive the evolution of viral sequences in humans. These selection forces include pressure on additional, overlapping, or adjacent T cell epitopes that are presented in the context of other MHC class I and II alleles as well as selection pressure to preserve viral replication fitness (45). Because the HCV NS31073–1082 sequence is located directly downstream of the HCV protease domain, it is, for example, possible that the Y/F mutation affects HCV replication and was therefore also found in HLA-A2–negative patients. For these reasons, the HLA-A2–transgenic mouse model provides a valuable tool for analyzing the effect of a single mutation on the induction of epitope-specific CD8+ cells in the context of a single, defined HLA molecule.

Although this study demonstrated a mechanism of altered antigen processing and impaired induction of HCV-specific T cells, significantly larger, population-based studies will be required to analyze the contribution of this mechanism to the overall selection pressure that drives HCV sequence evolution in infected patients. As a starting point for these studies, mutations in the flanking region of this particular HCV NS31073–1081 epitope are intriguing for several reasons. In addition to being the single most vigorously recognized CD8+ T cell epitope in all published studies (4, 2025), the NS31073–1081 epitope is one of only a few described epitopes that are recognized by circulating T cells as well as by nonspecifically expanded intrahepatic T cells (46). Moreover, it is also one of only two epitopes for which a TCR antagonist based on an intraepitope mutation has been described in persistently infected patients (18). The Y/F mutation in the flanking region of the epitope described here should, however, not be regarded as a main cause of HCV persistence. Rather, it should be regarded as an example of a viral escape mechanism that might also occur in the flanking regions of other CD4+ and CD8+ T cell epitopes. If many of these mutations occur throughout the HCV polyprotein, they may collectively contribute to the evolution of HCV quasispecies in persistently infected patients and to the characteristic weakness of the HCV-specific immune response.

Methods

Clinical samples.

Sera of patients with persistent HCV infection were analyzed 18 years after an accidental single-source outbreak (genotype 1b, AJ32996) due to a contaminated anti–D immunoglobulin (20, 26). The patients gave informed consent to this analysis. Patient samples were analyzed at Medizinische Hochschule Hannover (MHH) under a protocol approved by the MHH Ethics Committee. At the time of analysis, none of the patients had developed liver cirrhosis. HCV persistence was defined by detection of serum HCV RNA by RT-PCR, and by detection of HCV antibodies by enzyme immunoassay (HCV Version 3.0; Abbott Diagnostika GmbH, Wiesbaden, Germany). HLA typing was performed with Terasaki HLA-typing trays (One Lambda Inc., Canoga Park, California, USA).

Peptides.

The wild-type NS3(Wt)1062–1095 VSTATQSFLATCVNGVCWTVYHGAGSKTLAGPKG polypeptide, the mutant NS3(Mut)1062–1095 VSTATQSFLATCVNGVCWTVFHGAGSKTLAGPKG polypeptide, the NS31062–1095 serine variants VSTATQSFLATSVNGVCWTVYHGAGSKTLAGPKG and VSTATQSFLATSVNGVCWTVFHGAGSKTLAGPKG, the NS31073–1081 epitope CVNGVCWTV and the corresponding carboxyterminally and/or amino-terminally elongated peptides, the NS31073–1081 serine variant SVNGVCWTV, and the control peptides vaccinia virusH3L SLSAYIIRV (47) and HCV NS31406–1415 KLVALGINAV were synthesized using standard Fmoc methodology on an Applied Biosystems 433A automated synthesizer at >90% purity (Applied Biosystems, Darmstadt, Germany).

Detection and sequencing of HCV.

Total RNA was extracted from 140 μl sera using QIAamp Viral RNA Kit (QIAGEN GmbH, Hilden, Germany), and reverse transcription was performed with Superscript II Reverse Transcriptase (Gibco BRL, Grand Island, New York, USA) and random hexamers (48, 49). HCV sequences from the highly conserved 5′ untranslated region were amplified with Taq Polymerase (Gibco BRL) in a nested PCR with primers HCV43S (CCCTGTGAGGAACT[AT]CTGTCTTCACGC) and HCV318AS (GGTGCACGGTCTACGAGACCT) and nested primers HCV78S (TCTAGCCATGGCGTTAGTCAG[CT]GA) and HCV288AS (CACTCGCAAGCACCCTATCAGGCAGT) (BioteZ Berlin-Buch GmbH, Berlin, Germany) in 1 cycle with 5 minutes at 93°C, 2 minutes at 52°C, 3 minutes at 72°C; in 35 cycles with 1 minute at 93°C, 1 minute at 52°C, 2 minutes at 72°C; and in 1 cycle with 4 minutes at 72°C. PCR products were visualized on an ethidium bromide–stained 1.5% agarose gel.

For sequencing analysis, reverse transcription of RNA isolated from the inoculum was performed with the HCV-specific primer NS3as (CAGCATGCCTCGTGACCA); reverse transcription of RNA isolated from the serum of persistently infected patients was performed with Superscript II Reverse Transcriptase (Gibco BRL) and random hexamers as previously described (48, 49). The NS3987–1133 region containing the NS31073–1081 epitope was amplified with primers NS3s (GAGGCCACTATGTCCAAATG) and NS3as (CAGCATGCCTCGTGACCA) and nested primers NS3s-nested (GTAGAGCCCGTCGTCTTCTC) and NS3as-nested (GCCTCGTGACCAAGTAAA-GG). PCR conditions for the outer primer pair were 1 cycle with 2 minutes at 94°C; 30 cycles with 30 seconds at 94°C, 90 seconds at 50°C, and 2 minutes at 72°C; and 1 cycle with 4 minutes at 72°C. PCR conditions for the inner primer pair were 1 cycle with 2 minutes at 94°C; 30 cycles with 30 seconds at 94°C, 90 seconds at 59°C, and 2 minutes at 72°C; and 1 cycle with 4 minutes at 72°C. PCR products were either sequenced directly using the ABI PRISM BigDye Terminator Cycle Ready Reaction Kit and the ABI PRISM 310 sequencer (PerkinElmer, Rodgau-Jügesheim, Germany) or cloned into pCRII Vector (Topo TA Cloning Kit; Invitrogen, Carlsbad, California, USA) for sequencing of molecular clones. Sequence analysis was performed with software Factura and Sequence Navigator (PerkinElmer).

Generation of recombinant vaccinia viruses encoding wild-type and mutant HCV NS3.

The HCV clone HCV-AD78P1 (Genbank number AJ132997) (50) was used as a template to amplify the full-length NS3 sequence by PCR using primers NS3FL-s (CCGCTAGCCACCATGGCGCCCATCACGGCCTATTCC; nt 3081–3101) and NS3FL-as (CCGCGGCCGCTTAGGTGACGACCTCCAGGTCAGC; nt 4974–4954) under the following PCR conditions: 1 cycle with 2 minutes at 95°C; 5 cycles with 30 seconds at 94°C, 1 minute at 52°C, and 4 minutes at 72°C; followed by 25 cycles with 30 seconds at 94°C, 1 minute at 60°C, and 4 minutes at 72°C; and 1 cycle with 4 minutes at 72°C. The primer pair included a KpnI (5′) and NotI (3′) restriction site, respectively, for cloning into the pEF1/myc-His vector (Invitrogen). Site-directed mutagenesis was performed with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, California, USA) and primers GCTGGAGTGTCTACCATGGCGCTGGC and GCCAGCGCCATGGTAGACAGTCCAGC to substitute the tyrosine for phenylalanine at position NS31082. NS3-sequences were then subcloned into plasmid p7.5K131 (51) using EcoRI restriction sites to generate the recombinant vaccinia viruses VVNS3-Wt and VVNS3-Mut by homologous recombination with the Copenhagen strain and selection on 143TK cells. Synthesis of HCV-NS3–specific mRNA was confirmed by Northern blot analysis of CV-1 cells infected with vaccinia viruses (MOI 10) using radioactively labeled HCV NS3-cDNA and standard techniques (52). HCV-NS3 expression of wild-type and mutant NS3-encoding vaccinia virus was comparable as determined by flow cytometry (not shown).

Proteasome purification and peptide digestion.

Constitutive 20S proteasomes were isolated from HepG2 human hepatoma cells and murine MEC-18 cells. Immunoproteasomes were isolated from HepG2 that had been cultured with 200 U/l human IFN-γ; (Roche Diagnostics GmbH, Mannheim, Germany) for 72 hours and from MEC-18 cells that had been transfected with LMP2, LMP7, and MECL-1 under a tetracycline-regulated promoter (10). The purity of isolated proteasomes was greater than 90% (11). Twenty micrograms of wild-type and mutant NS31062–1095 peptides were incubated with 2 μg purified proteasomes in 150 μl assay buffer (20 mM Hepes/KOH, pH 7.8, 2 mM MgAc2, 1 mM dithiothreitol) at 37°C. The reaction was terminated by the addition of 0.1% trifluoroacetic acid (TFA). Forty microliters of the digests were separated by reversed-phase chromatography on a μRPC C2/C18 2.1/10 column (Pharmacia Biotech, Freiburg, Germany) and analyzed online with an ion trap mass spectrometer (LCQ; Electron Corp., Dreieich, Germany) with an electrospray ion source. Peptides were identified by tandem mass spectrometry (MS/MS) experiments and the amount of generated NS31073–1081 epitope was calculated by comparison to a defined amount of synthetic NS31073–1081 peptide.

In vivo induction of HCV-specific CD8+ T cells in HLA-A2–transgenic mice.

AAD mice, which express the α1 and α2 domains of the HLA-A2.1 molecule and the α3 domain of the murine H-2Dd molecule (35), were used to test the in vivo effect of the HCV mutation on the induction of NS31073–1081-specific T cells. The animal protocol was approved by the National Institute for Diabetes, Digestive and Kidney Diseases (NIDDK) Animal Care and Use Committee.

For ex vivo analysis of NS31073–1081-specific T cells, 6- to 8-week-old AAD mice were intraperitoneally injected with 107 PFU recombinant vaccinia virus (VVNS3-Wt and VV NS3-Mut respectively) in 200 μl PBS. Spleens were isolated two weeks after immunization, injected with 400 μg/ml of Liberase CI (Roche Diagnostics, Indianapolis, Indiana, USA), incubated at 37°C for 30 min and forced through a cell strainer (Falcon; BD Biosciences, Franklin Lakes, New Jersey). Single-cell suspensions were subsequently incubated with purified anti-mouse CD16/CD32 (FcgIII/IIR; BD Pharmingen, San Diego, California, USA) for 15 minutes at 4°C, then washed with PBS/2% FBS and stained with the HLA-A2/HCV NS31073 tetramer (NIAID Tetramer Facility, Atlanta, Georgia, USA) for 30 minutes at room temperature. After two washes, cells were stained with FITC-conjugated anti-CD8 for an additional 30 minutes at 4°C, washed again, and resuspended in 500 ml PBS/0.5% paraformaldehyde (PFA) for flow cytometry.

For analysis of cytokine production, CD8+ T cells were isolated from spleen cells using MACS CD8+ T Cell Isolation Kit and Columns (Miltenyi Biotec, Auburn, California, USA), according to the manufacturer’s instructions. C1R-AAD cells (106) (35) that had been pulsed overnight with 10 mg/ml of either HCV NS31073–1081, VV H3L35–43, or HCV NS31406–1415 peptide were used to stimulate 2 × 106 purified CD8+ T cells for 12 hours in 1 ml RPMI1640 containing 10% fetal bovine serum (BioWhittaker, Walkersville, Maryland, USA), 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 50 μM 2-mercaptoethanol (standard medium), additionally supplemented with 50 U/ml IL-2, 10 mg/ml brefeldin A (BD Pharmingen), 2 ml anti-CD28, and 2 ml anti-CD49d. Cells were then pelleted, washed with PBS/2% FBS, and stained at 4°C for 30 minutes with FITC-conjugated anti-CD8 (BD Pharmingen). After two washes in PBS, cells were fixed with Cytofix/Cytoperm (BD Pharmingen) at 4°C for 30 minutes. Thereafter, cells were washed twice with 1× Perm/Wash buffer (BD Pharmingen) and stained at 4°C for 30 minutes with PE-conjugated anti–IFN-γ; or PE-conjugated IgG1 isotype control. After one additional wash in 1× Perm/Wash buffer, cells were resuspended in PBS and analyzed using a Becton-Dickinson FACSCalibur with CellQuest (BD, San Jose, California, USA) and FlowJo software (Tree Star, San Carlos, California, USA). At least 10,000 events were acquired in a forward and side-scatter gate set to exclude cell debris.

For the generation of T cell lines and for in vitro analysis of cytotoxicity, mice were immunized twice with 107 PFU recombinant vaccinia virus (VVNS3-Wt or VV NS3-Mut) in 200 μl PBS at 4-week intervals. An additional intramuscular immunization with NS3-Wt and NS3-Mut encoding pEF1/myc-His plasmids (Invitrogen) was performed prior to the vaccinia virus immunization in some experiments, but did not further enhance the NS31073–1081-specific T cell response. Spleen cells were harvested 7 days after the last immunization and stimulated in T-25 flasks (3 × 107 cells/flask) in standard medium containing 50 μg/ml synthetic NS31073–1081 peptide. On day 2 of culture, 10% Rat-T-Stim (Collaborative Biomedical Products, Bedford, Massachusetts, USA) was added. On day 7, a standard 51Cr release assay was performed using NS31073–1081-peptide–pulsed, 51Cr-labeled CIR-AAD cells as target cells, and a 40-fold excess of unlabeled CIR-AAD as previously described (36). Percent specific lysis was calculated as (experimental release – spontaneous release) ×100/(maximum release – spontaneous release), in which spontaneous and maximum release reflect target cell lysis in the absence of effector cells and in the presence of 10% Triton X-100 (Sigma-Aldrich, St. Louis, Missouri, USA), respectively. Nonspecific lysis in the absence of peptide was less than 10% in all assays.

Cytotoxicity assay using human T cell lines.

HCV NS31073–1081–specific T cell lines were used to detect the NS31073–1081 epitope in the proteasome digests. For this purpose, HCV NS31073–1081–specific, cytotoxic CD8+ T cell lines were established by repetitive NS31073–1081 peptide stimulation (20) from PBMCs of HCV-recovered patients. HCV-recovered patients were followed in the Liver Diseases Section, NIDDK, NIH, and gave informed consent according to a protocol approved by the NIDDK Institutional Review Board. HCV-specific T cell lines were tested in a serum-free 51Cr-release assay (20) against TAP-deficient T2 target cells that had either been loaded with 1 mM peptide overnight or incubated with 8-hour–proteasome digests for 12 hours in serum-free RPMI 1640 medium prior to labeling with 50 mCi 51Cr. Only NS31073–1081-specific T cell lines with a sensitivity level of at least 0.1 mM peptide were tested against target cells loaded with the proteasomal digests.

Statistical analysis.

Student’s t test (two-tailed) was used to compare the frequency of NS31073–1081-specific cells with their CTL activity in mice immunized with recombinant vaccinia virus expressing wild-type or mutant NS3, respectively. The Fisher exact probability test (two-tailed) was used to compare the frequency of the Y/F mutation in HLA-A2–positive and –negative patients.

Acknowledgments

The authors thank L. Müller and S. Bigl (Landesuntersuchungsanstalt, Chemnitz, Germany) for patient samples; M. Roggendorf (Universitätsklinikum Essen, Germany) for PCR products of the infectious source; and A. Elstner for expert technical assistance. The HLA-A2 tetramer was produced at the National Institute of Allergy and Infectious Diseases Tetramer Facility of the NIH AIDS Research and Reference Reagent Program. This study was supported by grant Se885/1-1 of the Deutsche Forschungsgemeinschaft, Bonn, Germany, to U. Seifert; grant 1297 (01KI 9659/5 Pathogenesis of HCV infection) of the Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (BMBF) to B. Rehermann; Sonderforschungsbereich 421 to P. Kloetzel and H. Hengel; and by the NIDDK, NIH, Department of Health and Human Services (DHHS) intramural research program.

Footnotes

Heiner Wedemeyer’s present address is: Department of Gastroenterology, Hepatology and Endocrinology, Medizinische Hochschule Hannover (MHH), Hannover, Germany.

Thomas Ruppert’s present address is: Zentrum für Molekulare Biologie Heidelberg, University of Heidelberg, Heidelberg, Germany.

Alice Sijts’s present address is: David H. Smith Center for Vaccine Biology and Immunology, Aab Institute for Biomedical Sciences, University of Rochester Medical Center, Rochester, New York, USA.

Nonstandard abbreviations used: hepatitis C virus (HCV); nonstructural protein 3 (NS3); phenylalanine (F); tyrosine (Y).

Conflict of interest: The authors have declared that no conflict of interest exists.

References

1. Shoukry N, et al. Memory CD8+ T cells are required for protection from persistent hepatitis C virus infection. J. Exp. Med. 2003;197:1645–1655. [PMC free article] [PubMed]
2. Grakoui A, et al. HCV persistence and immune evasion in the absence of memory T cell help. Science. 2003;302:659–662. [PubMed]
3. Diepolder HM, et al. Possible mechanism involving T lymphocyte response to non-structural protein 3 in viral clearance in acute hepatitis C virus infection. Lancet. 1995;346:1006–1007. [PubMed]
4. Lechner F, et al. CD8+ T lymphocyte responses are induced during acute hepatitis C virus infection but are not sustained. Eur. J. Immunol. 2000;30:2479–2487. [PubMed]
5. Thimme R, et al. Determinants of viral clearance and persistence during acute hepatitis C virus infection. J. Exp. Med. 2001;194:1395–1406. [PMC free article] [PubMed]
6. Pamer E, Cresswell P. Mechanisms of MHC class I-restricted antigen processing. Annu. Rev. Immunol. 1998;16:323–358. [PubMed]
7. Khan S, et al. Immunoproteasomes largely replace constitutive proteasomes during an antiviral and antibacterial immune response in the liver. J. Immunol. 2001;167:6859–6868. [PubMed]
8. Schwarz K, et al. Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope. J. Immunol. 2000;165:768–778. [PubMed]
9. Groettrup M, Khan S, Schwarz K, Schmidtke G. Interferon-gamma inducible exchanges of 20S proteasome active site subunits: why? Biochimie. 2001;83:367–372. [PubMed]
10. Sijts AJ, et al. Efficient generation of a hepatitis B virus cytotoxic T lymphocyte epitope requires the structural features of immunoproteasomes. J. Exp. Med. 2000;191:503–514. [PMC free article] [PubMed]
11. Groettrup M, et al. The interferon-gamma-inducible 11 S regulator (PA28) and the LMP2/LMP7 subunits govern the peptide production by the 20 S proteasome in vitro. J. Biol. Chem. 1995;270:23808–23815. [PubMed]
12. Sijts AJ, et al. MHC class I antigen processing of an adenovirus CTL epitope is linked to the levels of immunoproteasomes in infected cells. J. Immunol. 2000;164:4500–4506. [PubMed]
13. Kloetzel PM. Antigen processing by the proteasome. Nat. Rev. Mol. Cell Biol. 2001;2:179–187. [PubMed]
14. Neumann AU, et al. Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-alpha therapy. Science. 1998;282:103–107. [PubMed]
15. Weiner A, et al. Persistent hepatitis C virus infection in a chimpanzee is associated with emergence of a cytotoxic T lymphocyte escape variant. Proc. Natl. Acad. Sci. U. S. A. 1995;92:2755–2759. [PMC free article] [PubMed]
16. Erickson AL, et al. The outcome of hepatitis C virus infection is predicted by escape mutations in epitopes targeted by cytotoxic T lymphocytes. Immunity. 2001;15:883–895. [PubMed]
17. Farci P, et al. The outcome of acute hepatitis C predicted by the evolution of the viral quasispecies. Science. 2000;288:339–344. [PubMed]
18. Chang KM, et al. Immunological significance of cytotoxic T lymphocyte epitope variants in patients chronically infected by the hepatitis C virus. J. Clin. Invest. 1997;100:2376–2385. [PMC free article] [PubMed]
19. Tsai SL, et al. Hepatitis C virus variants circumventing cytotoxic T lymphocyte activity as a mechanism of chronicity. Gastroenterology. 1998;115:954–965. [PubMed]
20. Takaki A, et al. Cellular immune responses persist, humoral responses decrease two decades after recovery from a single source outbreak of hepatitis C. Nat. Med. 2000;6:578–582. [PubMed]
21. Lechner F, et al. Analysis of successful immune responses in persons infected with hepatitis C virus. J. Exp. Med. 2000;191:1499–1512. [PMC free article] [PubMed]
22. Gruener NH, et al. Association of hepatitis C virus-specific CD8+ T cells with viral clearance in acute hepatitis C. J. Infect. Dis. 2000;181:1528–1536. [PubMed]
23. Cerny A, et al. Cytotoxic T lymphocyte response to hepatitis C virus - derived peptides containing the HLA A2.1 binding motif. J. Clin. Invest. 1995;95:521–530. [PMC free article] [PubMed]
24. Cucchiarini M, et al. Vigorous peripheral blood cytotoxic T cell response during the acute phase of hepatitis C virus infection. Cell Immunol. 2000;203:111–123. [PubMed]
25. Prezzi C, et al. Virus-specific CD8(+) T cells with type 1 or type 2 cytokine profile are related to different disease activity in chronic hepatitis C virus infection. Eur. J. Immunol. 2001;31:894–906. [PubMed]
26. Wiese M, Berr F, Lafrenz M, Porst H, Oesen U. Low frequency of cirrhosis in a hepatitis C (genotype 1b) single-source outbreak in Germany: a 20-year multicenter study. Hepatology. 2000;32:91–96. [PubMed]
27. Wertheimer AM, et al. Novel CD4+ and CD8+ T-cell determinants within the NS3 protein in subjects with spontaneously resolved HCV infection. Hepatology. 2003;37:577–589. [PubMed]
28. Wedemeyer H, et al. Impaired effector function of hepatitis C virus-specific CD8+ T cells in chronic hepatitis C virus infection. J. Immunol. 2002;169:3447–3458. [PubMed]
29. Seifert U, et al. An essential role for tripeptidyl peptidase in the generation of an MHC class I epitope. Nat. Immunol. 2003;4:375–379. [PubMed]
30. Kuckelkorn U, et al. Link between organ-specific antigen processing by 20S proteasomes and CD8(+) T cell-mediated autoimmunity. J. Exp. Med. 2002;195:983–990. [PMC free article] [PubMed]
31. Mo XY, Cascio P, Lemerise K, Goldberg AL, Rock K. Distinct proteolytic processes generate the C and N termini of MHC class I-binding peptides. J. Immunol. 1999;163:5851–5859. [PubMed]
32. Beninga J, Rock KL, Goldberg AL. Interferon-gamma can stimulate post-proteasomal trimming of the N terminus of an antigenic peptide by inducing leucine aminopeptidase. J. Biol. Chem. 1998;273:18734–18742. [PubMed]
33. Niedermann G, et al. Contribution of proteasome-mediated proteolysis to the hierarchy of epitopes presented by major histocompatibility complex class I molecules. Immunity. 1995;2:289–299. [PubMed]
34. Craiu A, Akopian T, Goldberg A, Rock KL. Two distinct proteolytic processes in the generation of a major histocompatibility complex class I-presented peptide. Proc. Natl. Acad. Sci. U. S. A. 1997;94:10850–10855. [PMC free article] [PubMed]
35. Newberg MH, et al. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J. Immunol. 1996;156:2473–2480. [PubMed]
36. Wedemeyer H, et al. Oral immunization with HCV-NS3-transformed salmonella: induction of HCV-specific CTL in a transgenic mouse model. Gastroenterology. 2001;121:1158–1166. [PubMed]
37. Stoltze L, et al. Two new proteases in the MHC class I processing pathway. Nat. Immunol. 2000;1:413–418. [PubMed]
38. Bertoletti A, et al. Natural variants of cytotoxic epitopes are T-cell receptor antagonists for antiviral cytotoxic T cells. Nature. 1994;369:407–410. [PubMed]
39. Klenerman P, et al. Cytotoxic T-cell activity antagonized by naturally occurring HIV-1 Gag variants. Nature. 1994;369:403–407. [PubMed]
40. Del Val M, Schlicht HJ, Ruppert T, Reddehase MJ, Koszinowski UH. Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein. Cell. 1991;66:1145–1153. [PubMed]
41. Theobald M, et al. The sequence alteration associated with a mutational hotspot in p53 protects cells from lysis by cytotoxic T lymphocytes specific for a flanking peptide epitope. J. Exp. Med. 1998;188:1017–1028. [PMC free article] [PubMed]
42. Beekman NJ, et al. Abrogation of CTL epitope processing by single amino acid substitution flanking the C-terminal proteasome cleavage site. J. Immunol. 2000;164:1898–1905. [PubMed]
43. Moore CB, et al. Evidence of HIV-1 adaptation to HLA-restricted immune responses at a population level. Science. 2002;296:1439–1443. [PubMed]
44. Brander C, et al. Efficient processing of the immunodominant, HLA-A*0201-restricted human immunodeficiency virus type 1 cytotoxic T-lymphocyte epitope despite multiple variations in the epitope flanking sequences. J. Virol. 1999;73:10191–10198. [PMC free article] [PubMed]
45. Friedrich TC, et al. Reversion of CTL escape-variant immunodeficiency viruses in vivo. Nat. Med. 2004;10:275–281. [PubMed]
46. Koziel MJ, et al. HLA class I-restricted cytotoxic T lymphocytes specific for hepatitis C virus. Identification of multiple epitopes and characterization of patterns of cytokine release. J. Clin. Invest. 1995;96:2311–2321. [PMC free article] [PubMed]
47. Drexler I, et al. Identification of vaccinia virus epitope-specific HLA-A*0201-restricted T cells and comparative analysis of smallpox vaccines. Proc. Natl. Acad. Sci. U. S. A. 2003;100:217–222. [PMC free article] [PubMed]
48. Rehermann B, et al. Quantitative analysis of the peripheral blood cytotoxic T lymphocyte response, disease activity and viral load in patients with chronic hepatitis C virus infection. J. Clin. Invest. 1996;98:1432–1440. [PMC free article] [PubMed]
49. Rehermann B, et al. Differential cytotoxic T lymphocyte responsiveness to the hepatitis B and C viruses in chronically infected patients. J. Virol. 1996;70:7092–7102. [PMC free article] [PubMed]
50. Rispeter K, Lu M, Lechner S, Zibert A, Roggendorf M. Cloning and characterization of a complete open reading frame of the hepatitis C virus genome in only two cDNA fragments. J. Gen. Virol. 1997;78:2751–2759. [PubMed]
51. Schlicht HJ, Schaller H. The secretory core protein of human hepatitis B virus is expressed on the cell surface. J. Virol. 1989;63:5399–5404. [PMC free article] [PubMed]
52. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. Plainview, New York, USA. 37–84.

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