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Proc Natl Acad Sci U S A. Oct 11, 1994; 91(21): 9921–9925.
PMCID: PMC44929

A determination of the frequency of gene conversion in unmanipulated mouse sperm.

Abstract

Gene conversion, sometimes also called micro gene conversion or gene conversion-like events, has been proposed to act on a number of genes in higher eukaryotes, such as gamma-globin, beta-tubulin, major urinary protein, and amyloid A genes. In the immune system, immunoglobulin genes and major histocompatibility complex class I and class II genes have been implicated. The notion that integral segments of DNA have been transferred from one gene to another in these cases has, however, met considerable resistance. We have devised a PCR assay detecting only the molecule that results if the E beta d-derived fragment analogous to that introduced in the bm12 mutation is transferred to the A beta k gene. We have proceeded to analyze sperm from the F1 cross C3H/HeJ (haplotype k) x BALB/c (haplotype d). In our assay, we find that the frequency for conversion of this particular DNA segment is 2 x 10(-6). This frequency is relevant only in the germ line; when liver cells were tested as an example of somatic cells, no events were observed, implying a frequency of < 2 x 10(-8) in liver. Fragments > 100 bp seem to be possible to transfer in this conversion.

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Selected References

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  • Radding CM. Genetic recombination: strand transfer and mismatch repair. Annu Rev Biochem. 1978;47:847–880. [PubMed]
  • Slightom JL, Blechl AE, Smithies O. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes. Cell. 1980 Oct;21(3):627–638. [PubMed]
  • Baltimore D. Gene conversion: some implications for immunoglobulin genes. Cell. 1981 Jun;24(3):592–594. [PubMed]
  • Sullivan KF, Lau JT, Cleveland DW. Apparent gene conversion between beta-tubulin genes yields multiple regulatory pathways for a single beta-tubulin polypeptide isotype. Mol Cell Biol. 1985 Sep;5(9):2454–2465. [PMC free article] [PubMed]
  • Clark AJ, Chave-Cox A, Ma X, Bishop JO. Analysis of mouse major urinary protein genes: variation between the exonic sequences of group 1 genes and a comparison with an active gene out with group 1 both suggest that gene conversion has occurred between MUP genes. EMBO J. 1985 Dec 1;4(12):3167–3171. [PMC free article] [PubMed]
  • Lowell CA, Potter DA, Stearman RS, Morrow JF. Structure of the murine serum amyloid A gene family. Gene conversion. J Biol Chem. 1986 Jun 25;261(18):8442–8452. [PubMed]
  • Reynaud CA, Anquez V, Grimal H, Weill JC. A hyperconversion mechanism generates the chicken light chain preimmune repertoire. Cell. 1987 Feb 13;48(3):379–388. [PubMed]
  • Mellor AL, Weiss EH, Ramachandran K, Flavell RA. A potential donor gene for the bm1 gene conversion event in the C57BL mouse. Nature. 1983 Dec 22;306(5945):792–795. [PubMed]
  • Weiss E, Golden L, Zakut R, Mellor A, Fahrner K, Kvist S, Flavell RA. The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens. EMBO J. 1983;2(3):453–462. [PMC free article] [PubMed]
  • Widera G, Flavell RA. The nucleotide sequence of the murine I-E beta b immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex. EMBO J. 1984 Jun;3(6):1221–1225. [PMC free article] [PubMed]
  • Mengle-Gaw L, Conner S, McDevitt HO, Fathman CG. Gene conversion between murine class II major histocompatibility complex loci. Functional and molecular evidence from the bm 12 mutant. J Exp Med. 1984 Oct 1;160(4):1184–1194. [PMC free article] [PubMed]
  • McIntyre KR, Seidman JG. Nucleotide sequence of mutant I-A beta bm12 gene is evidence for genetic exchange between mouse immune response genes. Nature. 1984 Apr 5;308(5959):551–553. [PubMed]
  • Denaro M, Hammerling U, Rask L, Peterson PA. The Eb beta gene may have acted as the donor gene in a gene conversion-like event generating the Abm 12 beta mutant. EMBO J. 1984 Sep;3(9):2029–2032. [PMC free article] [PubMed]
  • Rubnitz J, Subramani S. Extrachromosomal and chromosomal gene conversion in mammalian cells. Mol Cell Biol. 1986 May;6(5):1608–1614. [PMC free article] [PubMed]
  • Letsou A, Liskay RM. Effect of the molecular nature of mutation on the efficiency of intrachromosomal gene conversion in mouse cells. Genetics. 1987 Dec;117(4):759–769. [PMC free article] [PubMed]
  • Murti JR, Bumbulis M, Schimenti JC. High-frequency germ line gene conversion in transgenic mice. Mol Cell Biol. 1992 Jun;12(6):2545–2552. [PMC free article] [PubMed]
  • Ollo R, Rougeon F. Gene conversion and polymorphism: generation of mouse immunoglobulin gamma 2a chain alleles by differential gene conversion by gamma 2b chain gene. Cell. 1983 Feb;32(2):515–523. [PubMed]
  • Maizels N. Might gene conversion be the mechanism of somatic hypermutation of mammalian immunoglobulin genes? Trends Genet. 1989 Jan;5(1):4–8. [PubMed]
  • Pease LR, Schulze DH, Pfaffenbach GM, Nathenson SG. Spontaneous H-2 mutants provide evidence that a copy mechanism analogous to gene conversion generates polymorphism in the major histocompatibility complex. Proc Natl Acad Sci U S A. 1983 Jan;80(1):242–246. [PMC free article] [PubMed]
  • Klein J. Gene conversion in MHC genes. Transplantation. 1984 Oct;38(4):327–329. [PubMed]
  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. [PubMed]
  • Li HH, Gyllensten UB, Cui XF, Saiki RK, Erlich HA, Arnheim N. Amplification and analysis of DNA sequences in single human sperm and diploid cells. Nature. 1988 Sep 29;335(6189):414–417. [PubMed]
  • Päbo S, Higuchi RG, Wilson AC. Ancient DNA and the polymerase chain reaction. The emerging field of molecular archaeology. J Biol Chem. 1989 Jun 15;264(17):9709–9712. [PubMed]
  • Nairn R, Yamaga K, Nathenson SG. Biochemistry of the gene products from murine MHC mutants. Annu Rev Genet. 1980;14:241–277. [PubMed]

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