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Journal List > Antimicrob Agents Chemother > v.4(1); Jul 1973

Formats:
Antimicrob Agents Chemother. 1973 July; 4(1): 11–15.
PMCID: PMC444497
Copyright notice
Separation and Detection of Tetracyclines by High-Speed Liquid Chromatography
A. G. Butterfield, D. W. Hughes, N. J. Pound, and W. L. Wilson
Drug Research Laboratories, Health Protection Branch, Department of National Health and Welfare, Ottawa, Ontario, Canada
Abstract
The high-speed liquid chromatographic behavior of 15 tetracyclines on anion- and cation-exchange columns is described and illustrated by typical separations of a number of tetracycline derivatives on several systems, as well as by the resolution of tetracycline from its degradation products epi-, epianhydro-, and anhydrotetracycline. These serve as a basis for a rapid, convenient, and precise method for the direct detection of the tetracycline antibiotics and their degradation products.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Ascione PP, Chrekian GP. Separation and determination of anhydrotetracycline, 4-epianhydrotetracycline, tetracycline, and 4-epitetracycline in a tetracycline mixture. J Pharm Sci. 1970 Oct;59(10):1480–1482. [PubMed]
  • Ascione PP, Zagar JB, Chrekian GP. An automated liquid chromatographic method for the determination of tetracycline antibiotics. J Chromatogr. 1972 Feb 23;65(2):377–386. [PubMed]
  • Ascione PP, Zagar JB, Chrekian GP. Tetracyclines I. Separation and examination by thin-layer chromatography. J Pharm Sci. 1967 Nov;56(11):1393–1404. [PubMed]
  • Caplis ME, Ragheb HS, Schall ED. Determination of tetracycline antibiotics by alternating-current polarography. J Pharm Sci. 1965 May;54(5):694–698. [PubMed]
  • Chatten LG, Krause SI. Colorimetric assay of tetracycline antibiotics. J Pharm Sci. 1971 Jan;60(1):107–110. [PubMed]
  • CHICCARELLI FS, WOOLFORD MH, Jr, AVERY ME. The spectrophotometric assay for chlortetracycline HCI and tetracycline HCI in pharmaceuticals. J Am Pharm Assoc Am Pharm Assoc (Baltim). 1959 May;48(5):263–268. [PubMed]
  • Comer JP, Comer I. Applications of thin-layer chromatography in pharmaceutical analyses. J Pharm Sci. 1967 Apr;56(4):413–436. [PubMed]
  • Dijkhuis IC, Brommet MR. Determination of epitetracycline and chlortetracycline in tetracycline by quantitative thin-layer chromatography. J Pharm Sci. 1970 Apr;59(4):558–560. [PubMed]
  • Alvarez Fernandez A, Torre Noceda V, Sanchez Carrera E. Simultaneous separation and quantitative determination of tetracycline, anhydrotetracycline, 4-epitetracycline, and 4-epi-anhydrotetracycline in degraded tetracyclines by thin-layer chromatography. J Pharm Sci. 1969 Apr;58(4):443–446. [PubMed]
  • Fike WW, Brake NW. Modified method for determining tetracycline, 4-epitetracycline, and anhydrotetracyclines in tetracycline base or hydrochloride. J Pharm Sci. 1972 Apr;61(4):615–617. [PubMed]
  • KELLY RG. DETERMINATION OF ANHYDROTETRACYCLINE AND 4-EPIANHYDROTETRACYCLINE IN A TETRACYCLINE MIXTURE. J Pharm Sci. 1964 Dec;53:1551–1552. [PubMed]
  • Kelly RG, Peets LM, Hoyt KD. A fluorometric method of analysis for tetracycline. Anal Biochem. 1969 Apr 4;28(1):222–229. [PubMed]
  • Lever M. Improved fluorometric determination of tetracyclines. Biochem Med. 1972 Jun;6(3):216–222. [PubMed]
  • Lloyd PB, Corford CC. A thin-layer chromatographic limit test for the detection of anhydrotetracycline and 4-epi-anhydrotetracycline in tetracycline. J Chromatogr. 1970 Dec;53(2):403–405. [PubMed]
  • Lodi L, Meinardi G, Rossi E. Chromatographic methods for the determination of tetracycline by-products. Farmaco Prat. 1969 Dec;24(12):759–765. [PubMed]
  • Pernarowski M, Searl RO, Naylor J. Application of absorbance ratios to analysis of pharmaceuticals. V. Analysis of tetracycline hydrochloride in presence of anhydrotetracycline and epianhydrotetracycline. J Pharm Sci. 1969 Apr;58(4):470–472. [PubMed]
  • Radecka C, Wilson WL. Direct densitometric determination of tetracycline in pharmaceutical preparations. J Chromatogr. 1971 May 6;57(2):297–302. [PubMed]
  • Simmons DL, Koorengevel CK, Kubelka R, Seers P. Quantitative analysis of anhydrotetracycline on microcrystalline cellulose. J Pharm Sci. 1966 Feb;55(2):219–220. [PubMed]
  • Simmons DL, Woo HS, Koorengevel CM, Seers P. Quantitative determination by thin-layer chromatography of anhydrotetracyclines in degraded tetracycline tablets. J Pharm Sci. 1966 Nov;55(11):1313–1315. [PubMed]
  • van Hoeck G, Kapétanidis I, Mirimanoff A. Séparation par chromatographie sur couche mince de la tétracycline, de l'anhydrotétracycline et de leurs épim'eres. Dosage fluorimétrique direct sur chromatoplaque. (Note préliminaire. Pharm Acta Helv. 1972 May;47(5):316–320. [PubMed]
  • Walton VC, Howlett MR, Selzer GB. Anhydrotetracycline and 4-epianhydrotetracycline in market tetracyclines and aged tetracycline products. J Pharm Sci. 1970 Aug;59(8):1160–1164. [PubMed]
  • Wilson DM, Lever M, Brosnan EA, Stillwell A. A simplified tetracycline assay. Clin Chim Acta. 1972 Jan;36(1):260–261. [PubMed]
  • YOKOYAMA F, CHATTEN LG. Nonaqueous titration of the tetracycline antibiotics and some commercial preparations. J Am Pharm Assoc Am Pharm Assoc (Baltim). 1958 Aug;47(8):548–553. [PubMed]
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