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J Clin Invest. Mar 1992; 89(3): 723–728.
PMCID: PMC442914

Monocyte chemotactic and activating factor is a potent histamine-releasing factor for basophils.

Abstract

Monocyte chemotactic and activating factor (MCAF) is a recently cloned cytokine that causes chemotaxis of basophils. In our pursuit of cytokines affecting basophil function, we studied the effect of MCAF on histamine secretion from basophils. Leukocytes from 20 donors, 10 allergic and 10 normal subjects, were studied. MCAF caused dose-dependent release of histamine at concentrations of 10(-8) and 10(-7) M, and the mean release was 31.25 +/- 2.9% at the highest concentration. In the same experiments the mean histamine release by anti-IgE and histamine releasing factor (HRF) was 27.05 +/- 4% and 32.70 +/- 2.7%, respectively. All 20 subjects responded to MCAF with significant histamine release. Allergic subjects released significantly more histamine than normals in response to anti-IgE (P less than 0.01) but not to MCAF (P = 0.2) and HRF (P = 0.1). The histamine release was significantly correlated between MCAF and HRF (P less than 0.01), but not between MCAF and anti-IgE (P greater than 0.05). The histamine release by MCAF was complete within the first 3 min. MCAF-induced degranulation was a calcium-dependent process. Leukocytes depleted of monocytes responded equally well to MCAF. Using an anti-MCAF affinity column we determined that greater than 50% of HRF activity of crude PBMC supernatant could be attributed to MCAF. Thus, we established that MCAF is a potent secretagogue for basophils.

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Selected References

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  • Kay AB, Ying S, Varney V, Gaga M, Durham SR, Moqbel R, Wardlaw AJ, Hamid Q. Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects. J Exp Med. 1991 Mar 1;173(3):775–778. [PMC free article] [PubMed]
  • Alam R, Welter JB, Forsythe PA, Lett-Brown MA, Grant JA. Comparative effect of recombinant IL-1, -2, -3, -4, and -6, IFN-gamma, granulocyte-macrophage-colony-stimulating factor, tumor necrosis factor-alpha, and histamine-releasing factors on the secretion of histamine from basophils. J Immunol. 1989 May 15;142(10):3431–3435. [PubMed]
  • Baeza ML, Reddigari SR, Kornfeld D, Ramani N, Smith EM, Hossler PA, Fischer T, Castor CW, Gorevic PG, Kaplan AP. Relationship of one form of human histamine-releasing factor to connective tissue activating peptide-III. J Clin Invest. 1990 May;85(5):1516–1521. [PMC free article] [PubMed]
  • Lett-Brown MA, Boetcher DA, Leonard EJ. Chemotactic responses of normal human basophils to C5a and to lymphocyte-derived chemotactic factor. J Immunol. 1976 Jul;117(1):246–252. [PubMed]
  • Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 1989 Feb 27;244(2):487–493. [PubMed]
  • Furutani Y, Nomura H, Notake M, Oyamada Y, Fukui T, Yamada M, Larsen CG, Oppenheim JJ, Matsushima K. Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF). Biochem Biophys Res Commun. 1989 Feb 28;159(1):249–255. [PubMed]
  • Alam R, Forsythe PA, Lett-Brown MA, Grant JA. Cellular origin of histamine-releasing factor produced by peripheral blood mononuclear cells. J Immunol. 1989 Jun 1;142(11):3951–3956. [PubMed]
  • Pruzansky JJ, Grammer LC, Patterson R, Roberts M. Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release. J Immunol. 1983 Oct;131(4):1949–1953. [PubMed]
  • Leonard EJ, Yoshimura T. Human monocyte chemoattractant protein-1 (MCP-1). Immunol Today. 1990 Mar;11(3):97–101. [PubMed]
  • MacDonald SM, Lichtenstein LM, Proud D, Plaut M, Naclerio RM, MacGlashan DW, Kagey-Sobotka A. Studies of IgE-dependent histamine releasing factors: heterogeneity of IgE. J Immunol. 1987 Jul 15;139(2):506–512. [PubMed]
  • Alam R, Forsythe PA, Rankin JA, Boyars MC, Lett-Brown MA, Grant JA. Sensitivity of basophils to histamine releasing factor(s) of various origin: dependency on allergic phenotype of the donor and surface-bound IgE. J Allergy Clin Immunol. 1990 Jul;86(1):73–81. [PubMed]
  • Baeza ML, Reddigari S, Haak-Frendscho M, Kaplan AP. Purification and further characterization of human mononuclear cell histamine-releasing factor. J Clin Invest. 1989 Apr;83(4):1204–1210. [PMC free article] [PubMed]
  • Haak-Frendscho M, Arai N, Arai K, Baeza ML, Finn A, Kaplan AP. Human recombinant granulocyte-macrophage colony-stimulating factor and interleukin 3 cause basophil histamine release. J Clin Invest. 1988 Jul;82(1):17–20. [PMC free article] [PubMed]
  • MacDonald SM, Schleimer RP, Kagey-Sobotka A, Gillis S, Lichtenstein LM. Recombinant IL-3 induces histamine release from human basophils. J Immunol. 1989 May 15;142(10):3527–3532. [PubMed]
  • Zachariae CO, Anderson AO, Thompson HL, Appella E, Mantovani A, Oppenheim JJ, Matsushima K. Properties of monocyte chemotactic and activating factor (MCAF) purified from a human fibrosarcoma cell line. J Exp Med. 1990 Jun 1;171(6):2177–2182. [PMC free article] [PubMed]
  • Gallin JI, Wright DG, Schiffmann E. Role of secretory events in modulating human neutrophil chemotaxis. J Clin Invest. 1978 Dec;62(6):1364–1374. [PMC free article] [PubMed]
  • Naclerio RM, Proud D, Togias AG, Adkinson NF, Jr, Meyers DA, Kagey-Sobotka A, Plaut M, Norman PS, Lichtenstein LM. Inflammatory mediators in late antigen-induced rhinitis. N Engl J Med. 1985 Jul 11;313(2):65–70. [PubMed]
  • Warner JA, Pienkowski MM, Plaut M, Norman PS, Lichtenstein LM. Identification of histamine releasing factor(s) in the late phase of cutaneous IgE-mediated reactions. J Immunol. 1986 Apr 1;136(7):2583–2587. [PubMed]
  • Alam R, Welter J, Forsythe PA, Lett-Brown MA, Rankin JA, Boyars M, Grant JA. Detection of histamine release inhibitory factor- and histamine releasing factor-like activities in bronchoalveolar lavage fluids. Am Rev Respir Dis. 1990 Mar;141(3):666–671. [PubMed]
  • Alam R, Kuna P, Rozniecki J, Kuzminska B. The magnitude of the spontaneous production of histamine-releasing factor (HRF) by lymphocytes in vitro correlates with the state of bronchial hyperreactivity in patients with asthma. J Allergy Clin Immunol. 1987 Jan;79(1):103–108. [PubMed]
  • Sampson HA, Broadbent KR, Bernhisel-Broadbent J. Spontaneous release of histamine from basophils and histamine-releasing factor in patients with atopic dermatitis and food hypersensitivity. N Engl J Med. 1989 Jul 27;321(4):228–232. [PubMed]

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