Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. 1994 Jun 21; 91(13): 5922–5926.
PMCID: PMC44109

Resistance-associated point mutations in insecticide-insensitive acetylcholinesterase.

Abstract

Extensive utilization of pesticides against insects provides us with a good model for studying the adaptation of a eukaryotic genome to a strong selective pressure. One mechanism of resistance is the alteration of acetylcholinesterase (EC 3.1.1.7), the molecular target for organophosphates and carbamates. Here, we report the sequence analysis of the Ace gene in several resistant field strains of Drosophila melanogaster. This analysis resulted in the identification of five point mutations associated with reduced sensitivities to insecticides. In some cases, several of these mutations were found to be combined in the same protein, leading to different resistance patterns. Our results suggest that recombination between resistant alleles preexisting in natural populations is a mechanism by which insects rapidly adapt to new selective pressures.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (947K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Mouchès C, Pasteur N, Bergé JB, Hyrien O, Raymond M, de Saint Vincent BR, de Silvestri M, Georghiou GP. Amplification of an esterase gene is responsible for insecticide resistance in a California Culex mosquito. Science. 1986 Aug 15;233(4765):778–780. [PubMed]
  • Field LM, Devonshire AL, Forde BG. Molecular evidence that insecticide resistance in peach-potato aphids (Myzus persicae Sulz.) results from amplification of an esterase gene. Biochem J. 1988 Apr 1;251(1):309–312. [PMC free article] [PubMed]
  • Wang JY, McCommas S, Syvanen M. Molecular cloning of a glutathione S-transferase overproduced in an insecticide-resistant strain of the housefly (Musca domestica). Mol Gen Genet. 1991 Jun;227(2):260–266. [PubMed]
  • Fournier D, Bride JM, Poirie M, Bergé JB, Plapp FW., Jr Insect glutathione S-transferases. Biochemical characteristics of the major forms from houseflies susceptible and resistant to insecticides. J Biol Chem. 1992 Jan 25;267(3):1840–1845. [PubMed]
  • Fournier D, Bride JM, Hoffmann F, Karch F. Acetylcholinesterase. Two types of modifications confer resistance to insecticide. J Biol Chem. 1992 Jul 15;267(20):14270–14274. [PubMed]
  • Ffrench-Constant RH, Rocheleau TA, Steichen JC, Chalmers AE. A point mutation in a Drosophila GABA receptor confers insecticide resistance. Nature. 1993 Jun 3;363(6428):449–451. [PubMed]
  • ALDRIDGE WN. Some properties of specific cholinesterase with particular reference to the mechanism of inhibition by diethyl p-nitrophenyl thiophosphate (E 605) and analogues. Biochem J. 1950 Apr;46(4):451–460. [PMC free article] [PubMed]
  • Pralavorio M, Fournier D. Drosophila acetylcholinesterase: characterization of different mutants resistant to insecticides. Biochem Genet. 1992 Feb;30(1-2):77–83. [PubMed]
  • Fournier D, Karch F, Bride JM, Hall LM, Bergé JB, Spierer P. Drosophila melanogaster acetylcholinesterase gene. Structure, evolution and mutations. J Mol Biol. 1989 Nov 5;210(1):15–22. [PubMed]
  • Hall LM, Spierer P. The Ace locus of Drosophila melanogaster: structural gene for acetylcholinesterase with an unusual 5' leader. EMBO J. 1986 Nov;5(11):2949–2954. [PMC free article] [PubMed]
  • Mutero A, Fournier D. Post-translational modifications of Drosophila acetylcholinesterase. In vitro mutagenesis and expression in Xenopus oocytes. J Biol Chem. 1992 Jan 25;267(3):1695–1700. [PubMed]
  • Nakamaye KL, Eckstein F. Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis. Nucleic Acids Res. 1986 Dec 22;14(24):9679–9698. [PMC free article] [PubMed]
  • Fournier D, Mutero A, Rungger D. Drosophila acetylcholinesterase. Expression of a functional precursor in Xenopus oocytes. Eur J Biochem. 1992 Feb 1;203(3):513–519. [PubMed]
  • Sussman JL, Harel M, Frolow F, Oefner C, Goldman A, Toker L, Silman I. Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein. Science. 1991 Aug 23;253(5022):872–879. [PubMed]
  • Vellom DC, Radić Z, Li Y, Pickering NA, Camp S, Taylor P. Amino acid residues controlling acetylcholinesterase and butyrylcholinesterase specificity. Biochemistry. 1993 Jan 12;32(1):12–17. [PubMed]
  • Mutero A, Pralavorio M, Simeon V, Fournier D. Catalytic properties of cholinesterases: importance of tyrosine 109 in Drosophila protein. Neuroreport. 1992 Jan;3(1):39–42. [PubMed]
  • Radić Z, Gibney G, Kawamoto S, MacPhee-Quigley K, Bongiorno C, Taylor P. Expression of recombinant acetylcholinesterase in a baculovirus system: kinetic properties of glutamate 199 mutants. Biochemistry. 1992 Oct 13;31(40):9760–9767. [PubMed]
  • King JL, Jukes TH. Non-Darwinian evolution. Science. 1969 May 16;164(3881):788–798. [PubMed]
  • Nagoshi RN, Gelbart WM. Molecular and recombinational mapping of mutations in the Ace locus of Drosophila melanogaster. Genetics. 1987 Nov;117(3):487–502. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • Gene
    Gene
    Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
  • GEO Profiles
    GEO Profiles
    Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
  • HomoloGene
    HomoloGene
    HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...