Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. 1974 Sep; 71(9): 3672–3676.
PMCID: PMC433838

Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall Tumors


Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 104-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per three diploid tumor cells).

By the same technique, PS8 bacteriophage DNA is not detected in crown gall tumor DNA under conditions that allow detection of 0.0007-0.001% added phage DNA. Crown gall tumor DNA cannot contain as much as one entire phage genome per diploid tumor cell. The presence of a small fraction of either genome (less than 5%), even if present as multiple copies, would escape detection by this method.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Sacristán MD, Melchers G. The caryological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Mol Gen Genet. 1969;105(4):317–333. [PubMed]
  • Schilperoort RA, Veldstra H, Warnaar SO, Mulder G, Cohen JA. Formation of complexes between DNA isolated from tobacco crown gall tumours and RNA complementary to Agrobacterium tumefaciens DNA. Biochim Biophys Acta. 1967 Sep 26;145(2):523–525. [PubMed]
  • Quétier F, Huguet T, Guillé E. Induction of Crown-gall: partial homology between tumor-cell DNA, bacterial DNA and the G+C--rich DNA of stressed normal cells. Biochem Biophys Res Commun. 1969 Jan 6;34(1):128–133. [PubMed]
  • Srivastava BI. DNA-DNA hybridization studies between bacterial DNA, crown gall tumor cell DNA and the normal cell DNA. Life Sci II. 1970 Aug 8;9(15):889–892. [PubMed]
  • Schilperoort RA, Van Sittert NJ, Schell J. The presence of both phage PS8 and Agrobacterium tumefaciens A 6 DNA base sequences in A 6 -induced sterile crown-gall tissue cultured in vitro. Eur J Biochem. 1973 Feb 15;33(1):1–7. [PubMed]
  • Milo GE, Srivastava BI. RNA-DNA hybridization studies with the crown gall bacteria and the tobacco tumor tissue. Biochem Biophys Res Commun. 1969 Jan 27;34(2):196–199. [PubMed]
  • Schilperoort RA, Meijs WH, Pippel GMW, Veldstra H. Agrobacterium tumefaciens cross-reacting antigens in sterile crown-gall tumors. FEBS Lett. 1969 May;3(3):173–176. [PubMed]
  • Chadha KC, Srivastava BI. Evidence for the Presence of Bacteria-specific Proteins in Sterile Crown Gall Tumor Tissue. Plant Physiol. 1971 Aug;48(2):125–129. [PMC free article] [PubMed]
  • Parsons CL, Beardsley RE. Bacteriphage activity in homogenates of crown gall tissue. J Virol. 1968 Jun;2(6):651–651. [PMC free article] [PubMed]
  • Kurkdjian A. Observations sur la présence des phages dans les plaies infectées par différentes souches d'Agrobacterium tumefaciens (Smith et Town) Conn. Ann Inst Pasteur (Paris) 1970 May;118(5):690–696. [PubMed]
  • Westphal H, Dulbecco R. Viral DNA in polyoma- and SV40-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1158–1165. [PMC free article] [PubMed]
  • Gelb LD, Kohne DE, Martin MA. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971 Apr 14;57(1):129–145. [PubMed]
  • Pettersson U, Sambrook J. Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973 Jan;73(1):125–130. [PubMed]
  • Ozanne B, Sharp PA, Sambrook J. Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA. J Virol. 1973 Jul;12(1):90–98. [PMC free article] [PubMed]
  • Nonoyama M, Pagano JS. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature. 1973 Mar 2;242(5392):44–47. [PubMed]
  • Johnson R, Guderian RH, Eden F, Chilton MD, Gordon MP, Nester EW. Detection and quantitation of octopine in normal plant tissue and in crown gall tumors. Proc Natl Acad Sci U S A. 1974 Feb;71(2):536–539. [PMC free article] [PubMed]
  • Hamilton RH, Künsch U, Temperli A. Simple rapid procedures for isolation of tobacco leaf nuclei. Anal Biochem. 1972 Sep;49(1):48–57. [PubMed]
  • Whiteley AH, McCarthy BJ, Whiteley HR. Changing populations of messenger RNA during sea urchin development. Proc Natl Acad Sci U S A. 1966 Mar;55(3):519–525. [PMC free article] [PubMed]
  • Hill EB, Wayne LG, Gross WM. Purification of mycobacterial deoxyribonucleic acid. J Bacteriol. 1972 Dec;112(3):1033–1039. [PMC free article] [PubMed]
  • BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. [PMC free article] [PubMed]
  • Britten RJ, Kohne DE. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. [PubMed]
  • McConaughy BL, Laird CD, McCarthy BJ. Nucleic acid reassociation in formamide. Biochemistry. 1969 Aug;8(8):3289–3295. [PubMed]
  • Tibbetts C, Johansson K, Philipson L. Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. J Virol. 1973 Aug;12(2):218–225. [PMC free article] [PubMed]
  • Siegel A, Lightfoot D, Ward OG, Keener S. DNA Complementary to Ribosomal RNA: Relation between Genomic Proportion and Ploidy. Science. 1973 Feb 16;179(4074):682–683. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...