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Proc Natl Acad Sci U S A. May 1973; 70(5): 1316–1320.
PMCID: PMC433487

Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis


A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.

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  • Teng CS, Teng CT, Allfrey VG. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription. J Biol Chem. 1971 Jun 10;246(11):3597–3609. [PubMed]
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  • Balhorn R, Balhorn M, Morris HP, Chalkley R. Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate. Cancer Res. 1972 Aug;32(8):1775–1784. [PubMed]

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