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Proc Natl Acad Sci U S A. May 1973; 70(5): 1316–1320.
PMCID: PMC433487

Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis

Abstract

A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.

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Selected References

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  • Teng CS, Teng CT, Allfrey VG. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription. J Biol Chem. 1971 Jun 10;246(11):3597–3609. [PubMed]
  • Elgin SC, Bonner J. Limited heterogeneity of the major nonhistone chromosomal proteins. Biochemistry. 1970 Oct 27;9(22):4440–4447. [PubMed]
  • Knecht ME, Busch H. Isolation of an electrophoretically homogeneous nonhistone nucleolar protein. Life Sci II. 1971 Nov 22;10(22):1297–1309. [PubMed]
  • Martini OH, Gould HJ. Enumeration of rabbit reticulocyte ribosomal proteins. J Mol Biol. 1971 Dec 14;62(2):403–405. [PubMed]
  • Kaltschmidt E, Wittmann HG. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. [PubMed]
  • Sherton CC, Wool IG. Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem. 1972 Jul 25;247(14):4460–4467. [PubMed]
  • Balhorn R, Balhorn M, Morris HP, Chalkley R. Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate. Cancer Res. 1972 Aug;32(8):1775–1784. [PubMed]

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