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Proc Natl Acad Sci U S A. Aug 1975; 72(8): 3235–3239.
PMCID: PMC432957

Transient response to chemotactic stimuli in Escherichia coli.

Abstract

We have followed by eye and with the tracking microscope the rotational behavior of E. coli tethered to coverslips by their flagella. The cells change their directions of rotation at random, on the average about once a second. When an attractant is added or a repellent is subtracted, they spin clockwise (as viewed through the coverslip, i.e., along the flagellum toward the body) for many seconds, then counter-clockwise for many seconds, and then gradually resume their normal mode of behavior. The time interval between the onset of the stimulus and the clockwise to counter-clockwise transitiion is a linear function of the change in receptor occupancy. The cells adapt slowly at a constant rate to the addition of an attractant or the subtraction of a repellent. They adapt rapidly to the subtraction of an attractant or the addition of a repellent. Responses to mixed stimuli can be analyzed in terms of one equivalent stimulus.

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Selected References

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  • Berg HC, Brown DA. Chemotaxis in Escherichia coli analysed by three-dimensional tracking. Nature. 1972 Oct 27;239(5374):500–504. [PubMed]
  • Berg HC, Brown DA. Chemotaxis in Escherichia coli analyzed by three-dimensional tracking. Antibiot Chemother (1971) 1974;19:55–78. [PubMed]
  • Brown DA, Berg HC. Temporal stimulation of chemotaxis in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1388–1392. [PMC free article] [PubMed]
  • Macnab RM, Koshland DE., Jr The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509–2512. [PMC free article] [PubMed]
  • Koshland DE., Jr Chemotaxis as a model for sensory systems. FEBS Lett. 1974 Mar 23;40(0):suppl–suppl:S9. [PubMed]
  • Silverman M, Simon M. Flagellar rotation and the mechanism of bacterial motility. Nature. 1974 May 3;249(452):73–74. [PubMed]
  • Larsen SH, Reader RW, Kort EN, Tso WW, Adler J. Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli. Nature. 1974 May 3;249(452):74–77. [PubMed]
  • Berg HC. Dynamic properties of bacterial flagellar motors. Nature. 1974 May 3;249(452):77–79. [PubMed]
  • Mesibov R, Adler J. Chemotaxis toward amino acids in Escherichia coli. J Bacteriol. 1972 Oct;112(1):315–326. [PMC free article] [PubMed]
  • Tso WW, Adler J. Negative chemotaxis in Escherichia coli. J Bacteriol. 1974 May;118(2):560–576. [PMC free article] [PubMed]
  • Hazelbauer GL, Mesibov RE, Adler J. Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1300–1307. [PMC free article] [PubMed]
  • Futrelle RP, Berg HC. Specification of gradients used for studies of chemotaxis. Nature. 1972 Oct 27;239(5374):517–518. [PubMed]
  • Berg HC. Bacterial behaviour. Nature. 1975 Apr 3;254(5499):389–392. [PubMed]
  • Spudich JL, Koshland DE., Jr Quantitation of the sensory response in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975 Feb;72(2):710–713. [PMC free article] [PubMed]
  • Mesibov R, Ordal GW, Adler J. The range of attractant concentrations for bacterial chemotaxis and the threshold and size of response over this range. Weber law and related phenomena. J Gen Physiol. 1973 Aug;62(2):203–223. [PMC free article] [PubMed]
  • Tsang N, Macnab R, Koshland DE., Jr Common mechanism for repellents and attractants in bacterial chemotaxis. Science. 1973 Jul 6;181(4094):60–63. [PubMed]
  • Adler J, Tso WW. "Decision"-making in bacteria: chemotactic response of Escherichia coli to conflicting stimuli. Science. 1974 Jun 21;184(4143):1292–1294. [PubMed]

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