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Journal List > Proc Natl Acad Sci U S A > v.72(6); Jun 1975

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Proc Natl Acad Sci U S A. 1975 June; 72(6): 2202–2206.
PMCID: PMC432725
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Mismatch repair in heteroduplex DNA.
J Wildenberg and M Meselson
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Abstract
DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one parental chain. (ii) The frequency of repair of a given mismatch to wild type depends on the marker, ranging from 3 to 20%. (iii) Excision tracts may extend several hundred nucleotides but are usually shorter than about 2000 nucleotides. (iv) In Rec-mediated bacteriophage crosses, recombination of markers closer than about 10-3 nucleotide pairs frequently occurs by mismatch repair within heteroduplex DNA. (V) The average amount of heteroduplex DNA formed in a Rec-mediated recombination event is a few thousand nucleotide pairs.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Whitehouse HL. Symposium No. 5: Genetic exchange. Introduction by the Chairman. Advances in recombination research. Genetics. 1974 Sep;78(1):237–245. [PubMed]
  • Hotchkiss RD. Molecular basis for genetic recombination. Genetics. 1974 Sep;78(1):247–257. [PubMed]
  • Holliday R. Molecular aspects of genetic exchange and gene conversion. Genetics. 1974 Sep;78(1):273–287. [PubMed]
  • Meselson MS, Radding CM. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. [PubMed]
  • Doerfler W, Hogness DS. Gene orientation in bacteriophage lambda as determined from the genetic activities of heteroduplex DNA formed in vitro. J Mol Biol. 1968 May 14;33(3):661–678. [PubMed]
  • White RL, Fox MS. On the molecular basis of high negative interference. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1544–1548. [PubMed]
  • Spatz HC, Trautner TA. One way to do experiments on gene conversion? Transfection with heteroduplex SPP1 DNA. Mol Gen Genet. 1970;109(1):84–106. [PubMed]
  • Baas PD, Jansz HS. Asymmetric information transfer during phi X174 DNA replication. J Mol Biol. 1972 Feb 14;63(3):557–568. [PubMed]
  • Roger M. Evidence for conversion of heteroduplex transforming DNAs to homoduplexes by recipient pneumococcal cells (DNA strand resolution-DNA repair-bacterial transformation-genetic recombination). Proc Natl Acad Sci U S A. 1972 Feb;69(2):466–470. [PubMed]
  • Appleyard RK. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. [PubMed]
  • Bachmann BJ. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. [PubMed]
  • Signer ER, Weil J. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination. J Mol Biol. 1968 Jul 14;34(2):261–271. [PubMed]
  • CAMPBELL A. The different kinds of transducing particles in the lambda-gal system. Cold Spring Harb Symp Quant Biol. 1958;23:83–84. [PubMed]
  • Manly KF, Signer ER, Radding CM. Nonessential functions of bacteriophage lambda. Virology. 1969 Feb;37(2):177–188. [PubMed]
  • SUSSMAN R, JACOB F. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage.]. C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517–1519. [PubMed]
  • KAISER AD, JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. [PubMed]
  • CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. [PubMed]
  • KAISER AD, HOGNESS DS. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960 Dec;2:392–415. [PubMed]
  • Russo VE. On the physical structure of lambda recombinant DNA. Mol Gen Genet. 1973 May 28;122(4):353–366. [PubMed]
  • Amati P, Meselson M. Localized Negative Interference in Bacteriophage. Genetics. 1965 Mar;51(3):369–379. [PubMed]
  • Felsenstein J. The evolutionary advantage of recombination. Genetics. 1974 Oct;78(2):737–756. [PubMed]
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