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Proc Natl Acad Sci U S A. Mar 1975; 72(3): 1007–1011.
PMCID: PMC432453

Isolation of nuclear pore complexes in association with a lamina.


Nuclear pore complexes have been isolated in association with a 150 A thick lamina by detergent and salt fractionation of nuclear envelopes from rat liver. The pore complexes exhibit characteristic morphology and appear to be attached in a highly specific orientation to the lamina, which extends over relatively large areas. The pore complex-lamina fraction is composed of three major and several minor polypeptides with little or no DNA, RNA, or phospholipid. It is suggested that the association of the pore complexes and the lamina reflects the structural arrangement of the nuclear periphery in vivo.

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Selected References

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  • CALLAN HG, TOMLIN SG. Experimental studies on amphibian oocyte nuclei. I. Investigation of the structure of the nuclear membrane by means of the electron microscope. Proc R Soc Lond B Biol Sci. 1950 Oct 13;137(888):367–378. [PubMed]
  • WATSON ML. The nuclear envelope; its structure and relation to cytoplasmic membranes. J Biophys Biochem Cytol. 1955 May 25;1(3):257–270. [PMC free article] [PubMed]
  • Wischnitzer S. The submicroscopic morphology of the interphase nucleus. Int Rev Cytol. 1973;34:1–48. [PubMed]
  • Kay RR, Johnston IR. The nuclear envelope: current problems of structure and of function. Subcell Biochem. 1973;2(2):127–167. [PubMed]
  • Abelson HT, Smith GH. Nuclear pores: the pore-annulus relationship in thin section. J Ultrastruct Res. 1970 Mar;30(5):558–588. [PubMed]
  • Kessel RG. Fine structure of the pore-annulus complex in the nuclear envelope and annulate lamellae of germ cells. Z Zellforsch Mikrosk Anat. 1969;94(4):441–453. [PubMed]
  • Franke WW. On the universality of nuclear pore complex structure. Z Zellforsch Mikrosk Anat. 1970;105(3):405–429. [PubMed]
  • Roberts K, Northcote DH. Structure of the nuclear pore in higher plants. Nature. 1970 Oct 24;228(5269):385–386. [PubMed]
  • MERRIAM RW. On the fine structure and composition of the nuclear envelope. J Biophys Biochem Cytol. 1961 Dec;11:559–570. [PMC free article] [PubMed]
  • Dupraw EJ. THE ORGANIZATION OF NUCLEI AND CHROMOSOMES IN HONEYBEE EMBRYONIC CELLS. Proc Natl Acad Sci U S A. 1965 Jan;53(1):161–168. [PMC free article] [PubMed]
  • Koshiba K, Smetana K, Busch H. On the ultrastructural cytochemistry of nuclear pores in Novikoff hepatoma cells. Exp Cell Res. 1970 May;60(2):199–209. [PubMed]
  • Beaulaton J. Sur l'action d'enzymes au niveau des pores nucléaires et d'autres structures de cellules sécrétrices prothoraciques incluses en épon. Z Zellforsch Mikrosk Anat. 1968;89(4):453–461. [PubMed]
  • Scheer U. Ultrastructure of the nuclear envelope of amphibian oocytes. IV. Chemical nature of the nuclear pore complex material. Z Zellforsch Mikrosk Anat. 1972;127(1):127–148. [PubMed]
  • Stevens BJ, Swift H. RNA transport from nucleus to cytoplasm in Chironomus salivary glands. J Cell Biol. 1966 Oct;31(1):55–77. [PMC free article] [PubMed]
  • Comings DE, Okada TA. Association of chromatin fibers with the annuli of the nuclear membrane. Exp Cell Res. 1970 Oct;62(2):293–302. [PubMed]
  • Fawcett DW. On the occurrence of a fibrous lamina on the inner aspect of the nuclear envelope in certain cells of vertebrates. Am J Anat. 1966 Jul;119(1):129–145. [PubMed]
  • Patrizi G, Poger M. The ultrastructure of the nuclear periphery. The zonula nucleum limitans. J Ultrastruct Res. 1967 Jan;17(1):127–136. [PubMed]
  • Aaronson RP, Blobel G. On the attachment of the nuclear pore complex. J Cell Biol. 1974 Sep;62(3):746–754. [PMC free article] [PubMed]
  • Blobel G, Potter VR. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. [PubMed]
  • Kay RR, Fraser D, Johnston IR. A method for the rapid isolation of nuclear membranes from rat liver. Characterisation of the membrane preparation and its associated DNA polymerase. Eur J Biochem. 1972 Oct 17;30(1):145–154. [PubMed]
  • BURTON K, PETERSEN GB. The quantitative distribution of pyrimidine nucleotides in calf thymus deoxyribonucleic acid. Biochim Biophys Acta. 1957 Dec;26(3):667–668. [PubMed]
  • Blobel G, Potter VR. Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimethable and ribosome fractions of rat liver after a single injection of lebaled orotic acid. Biochim Biophys Acta. 1968 Aug 23;166(1):48–57. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed]
  • Farquhar MG, Palade GE. Cell junctions in amphibian skin. J Cell Biol. 1965 Jul;26(1):263–291. [PMC free article] [PubMed]
  • LUFT JH. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. [PMC free article] [PubMed]
  • WATSON ML. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. [PMC free article] [PubMed]
  • Kashnig DM, Kasper CB. Isolation, morphology, and composition of the nuclear membrane from rat liver. J Biol Chem. 1969 Jul 25;244(14):3786–3792. [PubMed]
  • Berezney R, Funk LK, Crane FL. The isolation of nuclear membrane from a large-scale preparation of bovine liver nuclei. Biochim Biophys Acta. 1970 Jun 2;203(3):531–546. [PubMed]
  • Franke WW, Deumling B, Baerbelermen, Jarasch ED, Kleinig H. Nuclear membranes from mammalian liver. I. Isolation procedure and general characterization. J Cell Biol. 1970 Aug;46(2):379–395. [PMC free article] [PubMed]
  • Zentgraf H, Deumling B, Jarasch ED, Franke WW. Nuclear membranes and plasma membranes from hen erythrocytes. I. Isolation, characterization, and comparison. J Biol Chem. 1971 May 10;246(9):2986–2995. [PubMed]
  • Agutter PS. The isolation of the envelopes of rat liver nuclei. Biochim Biophys Acta. 1972 Jan 17;255(1):397–401. [PubMed]
  • Monneron A, Blobel G, Palade GE. Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes. J Cell Biol. 1972 Oct;55(1):104–125. [PMC free article] [PubMed]
  • Bornens M. Letter: Action of heparin on nuclei: solubilization of chromatin enabling the isolation of nuclear membranes. Nature. 1973 Jul 6;244(5410):28–30. [PubMed]
  • Hadjiolov AA, Tencheva ZS, Bojadjieva-Mikhailova AG. Isolation and some characteristics of cell nuclei from brain cortex of adult cat. J Cell Biol. 1965 Aug;26(2):383–393. [PMC free article] [PubMed]
  • Holtzman E, Smith I, Penman S. Electron microscopic studies of detergent-treated HeLa cell nuclei. J Mol Biol. 1966 May;17(1):131–135. [PubMed]
  • Sadowski PD, Steiner JW. Electron microscopic and biochemical characteristics of nuclei and nucleoli isolated from rat liver. J Cell Biol. 1968 Apr;37(1):147–161. [PMC free article] [PubMed]
  • Chardonnet Y, Dales S. Early events in the interaction of adenoviruses with HeLa cells. 3. Relationship between an ATPase activity in nuclear envelopes and transfer of core material: a hypothesis. Virology. 1972 May;48(2):342–359. [PubMed]
  • Teigler DJ, Baerwald RJ. A freeze-etch study of clustered nuclear pores. Tissue Cell. 1972;4(3):447–456. [PubMed]
  • Maul GG, Price JW, Lieberman MW. Formation and distribution of nuclear pore complexes in interphase. J Cell Biol. 1971 Nov;51(21):405–418. [PMC free article] [PubMed]
  • Northcote DH, Lewis DR. Freeze-etched surfaces of membranes and organelles in the cells of pea root tips. J Cell Sci. 1968 Jun;3(2):199–206. [PubMed]

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