• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jan 1975; 72(1): 306–309.
PMCID: PMC432293

Breaking of seed dormancy by catalase inhibition.

Abstract

Germination of some dormant seeds is promoted by solutions of thiourea, sodium nitrite, and hydroxylamine salts. The promotions are accompanied by irreversible inhibition of catalase (EC 1.11.1.6) in extracts from the seeds. The seeds are also promoted in germination by catechol and pyrogallol solutions. These effects are recorded for lettuce (Lactuca sativa L. cv. Grand Rapids) and pigweed (Amaranthus albus L.) seeds. The results indicae that metabolically derived hydrogen peroxide, spared from decomposition by catalase inhibition, oxidizes reduced NADPH required as the oxidant in the pentose pathway of glucose use. The metabolic system for such use of H2O2 involves the enzymes, peroxidase (EC 1.11.1.7) and pyridine nucleotide quinone oxidoreductase (EC 1.6.99.2), which are present in the dormant seed prior to imbibition of water.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (748K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Borthwick HA, Hendricks SB, Parker MW. The Reaction Controlling Floral Initiation. Proc Natl Acad Sci U S A. 1952 Nov;38(11):929–934. [PMC free article] [PubMed]
  • Hendricks SB, Taylorson RB. Promotion of seed germination by nitrate, nitrite, hydroxylamine, and ammonium salts. Plant Physiol. 1974 Sep;54(3):304–309. [PMC free article] [PubMed]
  • Böhlen P, Stein S, Dairman W, Udenfriend S. Fluorometric assay of proteins in the nanogram range. Arch Biochem Biophys. 1973 Mar;155(1):213–220. [PubMed]
  • Thompson RC, Kosar WF. STIMULATION OF GERMINATION OF DORMANT LETTUCE SEED BY SULPHUR COMPOUNDS. Plant Physiol. 1939 Jul;14(3):567–573. [PMC free article] [PubMed]
  • Longo GP, Dragonetti C, Longo CP. Cytochemical localization of catalase in glyoxysomes isolated from maize scutella. Plant Physiol. 1972 Oct;50(4):463–468. [PMC free article] [PubMed]
  • WOSILAIT WD, NASON A. Pyridine nucleotide-quinone reductase. I. Purification and properties of the enzyme from pea seeds. J Biol Chem. 1954 Jan;206(1):255–270. [PubMed]
  • CONN EE, KRAEMER LM, LIU PN, VENNESLAND B. The aerobic oxidation of reduced triphosphopyridine nucleotide by a wheat germ enzyme system. J Biol Chem. 1952 Jan;194(1):143–151. [PubMed]
  • Tezuka T, Yamamoto Y. Photoregulation of Nicotinamide Adenine Dinucleotide Kinase Activity in Cell-free Extracts. Plant Physiol. 1972 Oct;50(4):458–462. [PMC free article] [PubMed]
  • Frosch S, Wagner E, Mohr H. Control by phytochrome of the level of nicotinamide nucleotides in the cotyledons of the mustard seedling. Z Naturforsch C. 1974 Jul-Aug;29(7-8):392–398. [PubMed]
  • KEILIN D, HARTREE EF. Reactions of methaemoglobin and catalase with peroxides and hydrogen donors. Nature. 1954 Apr 17;173(4407):720–723. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...