• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jun 1977; 74(6): 2456–2460.
PMCID: PMC432191

Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.

Abstract

The insoluble granular material of the acrosome vesicle of sea urchin sperm has been isolated and shown to be a single 30,500 dalton protein for which the name "bindin" is proposed. The data presented are consistent with the hypothesis that bindin is the adhesive responsible for the attachment of sperm to the vitelline layer of the egg. Experimental results suggest that bindin may act by binding to carbohydrate receptors of vitelline layer glycoproteins. The speculation is made that sperm bindins may be the general mechanism by which animal sperm attach to eggs.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Summers RG, Hylander BL. Species-specificity of acrosome reaction and primary gamete binding in echinoids. Exp Cell Res. 1975 Nov;96(1):63–68. [PubMed]
  • Hartmann JF, Gwatkin RB, Hutchison CF. Early contact interactions between mammalian gametes in vitro: evidence that the vitellus influences adherence between sperm and zona pellucida. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2767–2769. [PMC free article] [PubMed]
  • Hartmann JF, Hutchison CF. Contact between hamster spermatozoa and the zona pellucida releases a factor which influences early binding stages. J Reprod Fertil. 1974 Mar;37(1):61–66. [PubMed]
  • Vacquier VD, Payne JE. Methods for quantitating sea urchin sperm-egg binding. Exp Cell Res. 1973 Nov;82(1):227–235. [PubMed]
  • Weber K, Pringle JR, Osborn M. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol. 1972;26:3–27. [PubMed]
  • Stegink LD, Meyer PD, Chalkley R. Acrylamide gel electrophoresis of hemoglobin polypeptide chains. Anal Biochem. 1971 Jun;41(2):351–359. [PubMed]
  • Ofek I, Mirelman D, Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • DAN J, OHORI Y, KUSHIDA H. STUDIES ON THE ACROSOME. VII. FORMATION OF THE ACROSOMAL PROCESS IN SEA URCHIN SPERMATOZOA. J Ultrastruct Res. 1964 Dec;11:508–524. [PubMed]
  • Longo FJ, Anderson E. Sperm differentiation in the sea urchins Arbacia punctulata and Strongylocentrotus purpuratus. J Ultrastruct Res. 1969 Jun;27(5):486–509. [PubMed]
  • Fairbanks G, Steck TL, Wallach DF. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. [PubMed]
  • Aketa K, Onitake K, Tsuzuki H. Tryptic disruption of sperm-binding site of sea urchin egg surface. Exp Cell Res. 1972 Mar;71(1):27–32. [PubMed]
  • Schmell E, Earles BJ, Breaux C, Lennarz WJ. Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata. J Cell Biol. 1977 Jan;72(1):35–46. [PMC free article] [PubMed]
  • Collins F. A reevaluation of the fertilizin hypothesis of sperm agglutination and the description of a novel form of sperm adhesion. Dev Biol. 1976 Apr;49(2):381–394. [PubMed]
  • Yen PH, Ballou CE. Structure and immunochemistry of Hansenula wingei Y-2340 mannan. Biochemistry. 1974 May 21;13(11):2420–2427. [PubMed]
  • Crandall MA, Brock TD. Molecular basis of mating in the yeast hansenula wingei. Bacteriol Rev. 1968 Sep;32(3):139–163. [PMC free article] [PubMed]
  • Wiese L, Wiese W. On sexual agglutination and mating type substances in isogamous dioecious chlamydomonads. IV. Unilateral inactivation of the sex contact capacity in compatible and incompatible taxa by alpha-mannosidase and snake venom protease. Dev Biol. 1975 Apr;43(2):264–276. [PubMed]
  • Knox RB, Clarke A, Harrison S, Smith P, Marchalonis JJ. Cell recognition in plants: Determinants of the stigma surface and their pollen interactions. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2788–2792. [PMC free article] [PubMed]
  • Turner RS, Burger MM. Involvement of a carbohydrate group in the active site for surface guided reassociation of animal cells. Nature. 1973 Aug 24;244(5417):509–510. [PubMed]
  • Müller WE, Müller I, Zahn RK, Kurelec B. Species-specific aggregation factor in sponges. VI. Aggregation receptor from the cell surface. J Cell Sci. 1976 Jul;21(2):227–241. [PubMed]
  • Rosen SD, Kafka JA, Simpson DL, Barondes SH. Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2554–2557. [PMC free article] [PubMed]
  • Rosen SD, Reitherman RW, Barondes SH. Distinct lectin activities from six species of cellular slime molds. Exp Cell Res. 1975 Oct 1;95(1):159–166. [PubMed]
  • Bowles DJ, Kauss H. Isolation of a lectin from liver plasma membrane and its binding to cellular membrane receptors in vitro. FEBS Lett. 1976 Jul 1;66(1):16–19. [PubMed]
  • Nowak TP, Haywood PL, Barondes SH. Developmentally regulated lectin in embryonic chick muscle and a myogenic cell line. Biochem Biophys Res Commun. 1976 Feb 9;68(3):650–657. [PubMed]
  • Brown GG. Scanning electron-microscopical and other observations of sperm fertilization reactions in Limulus polyphemus L. (Merostomata: Xiphosura). J Cell Sci. 1976 Dec;22(3):547–562. [PubMed]
  • Grey RD, Working PK, Hedrick JL. Evidence that the fertilization envelope blocks sperm entry in eggs of Xenopus laevis: interaction of sperm with isolated envelopes. Dev Biol. 1976 Nov;54(1):52–60. [PubMed]
  • Inoue M, Wolf DP. Sperm binding characteristics of the murine zona pellucida. Biol Reprod. 1975 Oct;13(3):340–346. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...