|
|
Proc Natl Acad Sci U S A. 1977 June; 74(6): 2389–2393. | PMCID: PMC432177 |
Terminally repeated sequences in the avian sarcoma virus RNA genome. M S Collett, P Dierks, J F Cahill, A J Faras, and J T Parsons Abstract The initiation of DNA synthesis in vitro by RNA-directed DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) of avian oncornaviruses requires a tRNAtrp primer molecule located close to the 5' end of the viral RNA genome. DNA transcripts, 100 nucleotides in length, initiated on the tRNAtrp primer molecule contain nucleotide sequences complementary to a large (25 nucleotides) RNase T1 oligonucleotide, T-13, located at the 5' terminus of the avian sarcoma virus RNA genome. tRNAtrp-initiated DNA transcripts with a length of about 70 nucleotides contain substantially fewer nucleotide sequences complementary to this 5'-terminal oligonucleotide, suggesting that the tRNAtrp primer associated with the avian sarcoma virus RNA is located approximately 100 nucleotides from the 5' end of the RNA. In addition, we present evidence to demonstrate that DNA transcribed from avian sarcoma virus RNA sequences located at the 3' end, immediately adjacent to the poly(A), contains nucleotide sequences that are complementary to the 5'-terminal T1 oligonucleotide T-13. These data indicate that the 5' end of the viral genome contains nucleotide sequences that are repeated at the 3' end of the genome. We conclude that the avian oncornavirus RNA genome is terminally redundant. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Varmus HE, Guntaka RV, Deng CT, Bishop JM. Synthesis, structure and function of avian sarcoma virus-specific DNA in permissive and nonpermissive cells. Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:987–996. [PubMed]
- Guntaka RV, Mahy BW, Bishop JM, Varmus HE. Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus. Nature. 1975 Feb 13;253(5492):507–511. [PubMed]
- Gianni AM, Smotkin D, Weinberg RA. Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus. Proc Natl Acad Sci U S A. 1975 Feb;72(2):447–451. [PubMed]
- Taylor JM, Illmensee R. Site on the RNA of an avian sarcoma virus at which primer is bound. J Virol. 1975 Sep;16(3):553–558. [PubMed]
- Staskus KA, Collett MS, Faras AJ. Initiation of DNA synthesis by the avian oncornavirus RNA-directed DNA polymerase: structural and functional localization of the major species of primer RNA on the oncornavirus genome. Virology. 1976 May;71(1):162–168. [PubMed]
- Dahlberg JE, Sawyer RC, Taylor JM, Faras AJ, Levinson WE, Goodman HM, Bishop JM. Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer. J Virol. 1974 May;13(5):1126–1133. [PubMed]
- Faras AJ, Taylor JM, Levinson WE, Goodman HM, Bishop JM. RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template. J Mol Biol. 1973 Sep 5;79(1):163–183. [PubMed]
- Faras AJ, Dahlberg JE, Sawyer RC, Harada F, Taylor JM, Levinson WE, Bishop JM, Goodman HM. Transcription of DNA from the 70S RNA of Rous sarcoma virus. II. Structure of a 4S RNA primer. J Virol. 1974 May;13(5):1134–1142. [PubMed]
- Collett MS, Faras AJ. Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1329–1332. [PubMed]
- Cashion LM, Joho RH, Planitz MA, Billeter MA, Weissmann C. Initiation sites of Rous sarcoma virus RNA-directed DNA synthesis in vitro. Nature. 1976 Jul 15;262(5565):186–190. [PubMed]
- Collett MS, Faras AJ. In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions. J Virol. 1977 Apr;22(1):86–96. [PubMed]
- Faras AJ, Taylor JM, McDonnell JP, Levinson WE, Bishop JM. Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus. Biochemistry. 1972 Jun 6;11(12):2334–2342. [PubMed]
- Billeter MA, Parsons JT, Coffin JM. The nucleotide sequence complexity of avian tumor virus RNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560–3564. [PubMed]
- Faras AJ, Levinson WE, Bishop JM, Goodman HM. Identification of a tRNA nucleotidyltransferase and its substrates in virions of avian RNA tumor viruses. Virology. 1974 Mar;58(1):126–135. [PubMed]
- Faras AJ, Dibble NA. RNA-directed DNA synthesis by the DNA polymerase of Rous sarcoma virus: structural and functional identification of 4S primer RNA in uninfected cells. Proc Natl Acad Sci U S A. 1975 Mar;72(3):859–863. [PubMed]
- Adams JM, Jeppesen PG, Sanger F, Barrell BG. Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA. Nature. 1969 Sep 6;223(5210):1009–1014. [PubMed]
- Coffin JM, Billeter MA. A physical map of the Rous sarcoma virus genome. J Mol Biol. 1976 Jan 25;100(3):293–318. [PubMed]
- Furuichi Y, Shatkin AJ, Stavnezer E, Bishop JM. Blocked, methylated 5'-terminal sequence in avian sarcoma virus RNA. Nature. 1975 Oct 16;257(5527):618–620. [PubMed]
- Rhodes DP, Banerjee AK. 5'-terminal sequence of vesicular stomatitis virus mRNA's synthesized in vitro. J Virol. 1975 Jan;17(1):33–42. [PubMed]
- Shine J, Czernilofsky AP, Friedrich R, Bishop JM, Goodman HM. Nucleotide sequence at the 5' terminus of the avian sarcoma virus genome. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1473–1477. [PubMed]
- Pérez-Bercoff R, Billeter MA. Characterization of the 3'-terminal region of the large molecular weight RNA subunits from normal and transformation-defective Rous sarcoma virus. Biochim Biophys Acta. 1976 Dec 1;454(2):383–388. [PubMed]
- Haseltine WA, Maxam AM, Gilbert W. Rous sarcoma virus genome is terminally redundant: the 5' sequence. Proc Natl Acad Sci U S A. 1977 Mar;74(3):989–993. [PubMed]
- Schwartz DE, Zamecnik PC, Weith HL. Rous sarcoma virus genome is terminally redundant: the 3' sequence. Proc Natl Acad Sci U S A. 1977 Mar;74(3):994–998. [PubMed]
|
This article has been 
