|
|
Proc Natl Acad Sci U S A. 1977 August; 74(8): 3171–3175. | PMCID: PMC431482 |
Spliced segments at the 5' terminus of adenovirus 2 late mRNA. S M Berget, C Moore, and P A Sharp Abstract An mRNA fraction coding for hexon polypeptide, the major virion structural protein, was purified by gel electrophoresis from extracts of adenovirus 2-infected cells late in the lytic cycle. The mRNA sequences in this fraction were mapped between 51.7 and 61.3 units on the genome by visualizing RNA-DNA hybrids in the electron microscope. When hybrids of hexon mRNA and single-stranded restriction endonuclease cleavage fragments of viral DNA were visualized in the electron microscope,branched forms were observed in which 160 nucleotides of RNA from the 5' terminus were not hydrogen bonded to the single-stranded DNA. DNA sequences complementary to the RNA sequences in each 5' tail were found by electron microscopy to be located at 17, 20, and 27 units on the same strand as that coding for the body of the hexon mRNA. Thus, four segments of viral RNA may be joined together during the synthesis of mature hexon mRNA. A model is presented for adenovirus late mRNA synthesis that involves multiple splicing during maturation of a larger precursor nuclear RNA. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Edmonds M, Vaughan MH, Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. [PubMed]
- Lee SY, Mendecki J, Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. [PubMed]
- Darnell JE, Wall R, Tushinski RJ. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. [PubMed]
- Furuichi Y, Morgan M, Muthukrishnan S, Shatkin AJ. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362–366. [PubMed]
- Wei CM, Moss B. Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014–3018. [PubMed]
- Philipson L, Wall R, Glickman G, Darnell JE. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806–2809. [PubMed]
- Hashimoto SI, Green M. Multiple methylated cap sequences in adenovirus type 2 early mRNA. J Virol. 1976 Nov;20(2):425–435. [PubMed]
- Moss B, Koczot F. Sequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA. J Virol. 1976 Feb;17(2):385–392. [PubMed]
- Lewis JB, Atkins JF, Anderson CW, Baum PR, Gesteland RF. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344–1348. [PubMed]
- Bachenheimer S, Darnell JE. Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4445–4449. [PubMed]
- Flint SJ, Sharp PA. Adenovirus transcription. V. Quantitation of viral RNA sequences in adenovirus 2-infected and transformed cells. J Mol Biol. 1976 Sep 25;106(3):749–774. [PubMed]
- Flint SJ, Gallimore PH, Sharp PA. Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cells. J Mol Biol. 1975 Jul 25;96(1):47–68. [PubMed]
- Lindberg U, Persson T, Philipson L. Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection. J Virol. 1972 Nov;10(5):909–919. [PubMed]
- Thomas M, White RL, Davis RW. Hybridization of RNA to double-stranded DNA: formation of R-loops. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2294–2298. [PubMed]
- Duesberg PH, Vogt PK. Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species. J Virol. 1973 Sep;12(3):594–599. [PubMed]
- Casey J, Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4(5):1539–1552. [PubMed]
- Green M. Oncogenic viruses. Annu Rev Biochem. 1970;39:701–756. [PubMed]
- White RL, Hogness DS. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA. Cell. 1977 Feb;10(2):177–192. [PubMed]
- Westphal H, Meyer J, Maizel JV., Jr Mapping of adenovirus messenger RNA by electron microscopy. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2069–2071. [PubMed]
- Lee CS, Davis RW, Davidson N. A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15. J Mol Biol. 1970 Feb 28;48(1):1–22. [PubMed]
- Robberson D, Aloni Y, Attardi G, Davidson N. Expression of the mitochondrial genome in HeLa cells. VI. Size determination of mitochondrial ribosomal RNA by electron microscopy. J Mol Biol. 1971 Sep 28;60(3):473–484. [PubMed]
- Parsons JT, Green M. Biochemical studies on adenovirus multiplication. 18. Resolution of early virus-specific RNA species in Ad 2 infected and transformed cells. Virology. 1971 Jul;45(1):154–162. [PubMed]
- Wall R, Philipson L, Darnell JE. Processing of adenovirus specific nuclear RNA during virus replication. Virology. 1972 Oct;50(1):27–34. [PubMed]
- McGuire PM, Swart C, Hodge LD. Adenovirus messenger RNA in mammalian cells: failure of polyribosome association in the absence of nuclear cleavage. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1578–1582. [PubMed]
- Goldberg S, Weber J, Darnell JE., Jr The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping by effects of ultraviolet irradiation. Cell. 1977 Apr;10(4):617–621. [PubMed]
- Perry RP, Kelley DE. Kinetics of formation of 5' terminal caps in mRNA. Cell. 1976 Jul;8(3):433–442. [PubMed]
|
This article has been 
