• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aacPermissionsJournals.ASM.orgJournalAAC ArticleJournal InfoAuthorsReviewers
Antimicrob Agents Chemother. Apr 1974; 5(4): 420–425.
PMCID: PMC428986

Studies on the Mechanism of Action of Miconazole: Effect of Miconazole on Respiration and Cell Permeability of Candida albicans

Abstract

The antifungal drug, miconazole nitrate, inhibits the growth of several species of Candida. Candida albicans, one of the pathogenic species, was totally inhibited at a concentration of approximately 10 μg/ml. Endogenous respiration was unaffected by the drug at a concentration as high as 100 μg/ml, whereas exogenous respiration was markedly sensitive and inhibited to an extent of 85%. The permeability of the cell membrane was changed as evidenced by the leakage of 260-nm absorbing materials, amino acids, proteins, and inorganic cations. The results we present clearly show that the drug alters the cellular permeability, and thus the exogenous respiration becomes sensitive to the drug.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (778K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ANAND N, DAVIS BD. Damage by streptomycin to the cell membrane of Escherichia coli. Nature. 1960 Jan 2;185:22–23. [PubMed]
  • Brugmans JP, Van Cutsem JM, Thienpont DC. Treatment of long-term tinea pedis with miconazole. Double-blind clinical evaluation. Arch Dermatol. 1970 Oct;102(4):428–432. [PubMed]
  • FEW AV, SCHULMAN JH. The absorption of polymyxin E by bacteria and bacterial cell walls and its bactericidal action. J Gen Microbiol. 1953 Dec;9(3):454–466. [PubMed]
  • Godefroi EF, Heeres J, Van Cutsem J, Janssen PA. The preparation and antimycotic properties of derivatives of 1-phenethylimidazole. J Med Chem. 1969 Sep;12(5):784–791. [PubMed]
  • Gottlieb D, Nicolas G. Mode of action of lomofungin. Appl Microbiol. 1969 Jul;18(1):35–40. [PMC free article] [PubMed]
  • Hugo WB, Bloomfield SF. Studies on the mode of action of the phenolic antibacterial agent fentichlor against Staphylococcus aureus and Escherichia coli. II. The effects of fentichlor on the bacterial membrane and the cytoplasmic constituents of the cell. J Appl Bacteriol. 1971 Sep;34(3):569–578. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • NEWTON BA. The release of soluble constituents from washed cells of Pseudomonas aeruginosa by the action of polymyxin. J Gen Microbiol. 1953 Aug;9(1):54–64. [PubMed]
  • Nose M, Arima K. On the mode of action of a new antifungal antibiotic, pyrrolnitrin. J Antibiot (Tokyo) 1969 Apr;22(4):135–143. [PubMed]
  • ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. [PubMed]
  • SALTON MRJ. The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects. J Gen Microbiol. 1951 May;5(2):391–404. [PubMed]
  • Sugawara S. Mode of action of azalomycin F. Effect of azalomycin F on Candida albicans. J Antibiot (Tokyo) 1967 Mar;20(2):93–108. [PubMed]
  • Thiery M, Mrozowski BJ, Van Kets H. Miconazole, a new broad-spectrum antimycotic, in the treatment of vaginal candidosis. Mykosen. 1972 Jan 1;15(1):35–37. [PubMed]
  • Vandaele R, Uyttendaele K. Miconazole nitrate in the topical treatment of dermatomycoses. A clinical evaluation. Arzneimittelforschung. 1972 Jul;22(7):1221–1223. [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...