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Proc Natl Acad Sci U S A. Jan 1972; 69(1): 258–263.
PMCID: PMC427587

Neurotransmitter Synthesis by Neuroblastoma Clones

Abstract

Neuroblastoma clones were examined for choline acetyltransferase (EC 2.3.1.6), tyrosine hydroxylase (EC 1.14.3.a), acetylcholinesterase (EC 3.1.1.7), and also for neurite formation. One clone does not form axons or dendrites. Three types of clones were found with respect to neurotransmitter synthesis: cholinergic, adrenergic, and clones that do not synthesize acetylcholine or catechols. All clones contain acetylcholinesterase. These results show that genes determining neurotransmitter species can be expressed in dividing cells, that the parental programs of gene expression are inherited, and that dividing cells can be programmed with respect to their ability to communicate with other cells.

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Selected References

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  • Augusti-Tocco G, Sato G. Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):311–315. [PMC free article] [PubMed]
  • Schubert D, Humphreys S, Baroni C, Cohn M. In vitro differentiation of a mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):316–323. [PMC free article] [PubMed]
  • Olmsted JB, Carlson K, Klebe R, Ruddle F, Rosenbaum J. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc Natl Acad Sci U S A. 1970 Jan;65(1):129–136. [PMC free article] [PubMed]
  • Nelson P, Ruffner W, Nirenberg M. Neuronal tumor cells with excitable membranes grown in vitro. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1004–1010. [PMC free article] [PubMed]
  • Seeds NW, Gilman AG, Amano T, Nirenberg MW. Regulation of axon formation by clonal lines of a neural tumor. Proc Natl Acad Sci U S A. 1970 May;66(1):160–167. [PMC free article] [PubMed]
  • Blume A, Gilbert F, Wilson S, Farber J, Rosenberg R, Nirenberg M. Regulation of acetylcholinesterase in neuroblastoma cells. Proc Natl Acad Sci U S A. 1970 Oct;67(2):786–792. [PMC free article] [PubMed]
  • Schrier BK, Shuster L. A simplified radiochemical assay for choline acetyltransferase. J Neurochem. 1967 Oct;14(10):977–985. [PubMed]
  • NAGATSU T, LEVITT M, UDENFRIEND S. A RAPID AND SIMPLE RADIOASSAY FOR TYROSINE HYDROXYLASE ACTIVITY. Anal Biochem. 1964 Sep;9:122–126. [PubMed]
  • Shiman R, Akino M, Kaufman S. Solubilization and partial purification of tyrosine hydroxylase from bovine adrenal medulla. J Biol Chem. 1971 Mar 10;246(5):1330–1340. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • NAGATSU T, LEVITT M, UDENFRIEND S. TYROSINE HYDROXYLASE. THE INITIAL STEP IN NOREPINEPHRINE BIOSYNTHESIS. J Biol Chem. 1964 Sep;239:2910–2917. [PubMed]
  • SCHNEIDER FH, GILLIS CN. CATECHOLAMINE BIOSYNTHESIS IN VIVO: AN APPLICATION OF THIN-LAYER CHROMATOGRAPHY. Biochem Pharmacol. 1965 Apr;14:623–626. [PubMed]
  • HAUSCHKA TS, KVEDAR BJ, GRINNELL ST, AMOS DB. Immunoselection of polyploids from predominantly diploid cell populations. Ann N Y Acad Sci. 1956 Mar 14;63(5):683–705. [PubMed]
  • BREGOFF HM, ROBERTS E, DELWICHE CC. Paper chromatography of quaternary ammonium bases and related compounds. J Biol Chem. 1953 Dec;205(2):565–574. [PubMed]

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