• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jan 31, 1995; 92(3): 791–795.
PMCID: PMC42706

Programmed DNA rearrangement of a cyanobacterial hupL gene in heterocysts.

Abstract

Programmed DNA rearrangements that occur during cellular differentiation are uncommon and have been described in only two prokaryotic organisms. Here, we identify the developmentally regulated rearrangement of a hydrogenase gene in heterocysts of the cyanobacterium Anabaena sp. strain PCC 7120. Heterocysts are terminally differentiated cells specialized for nitrogen fixation. Late during heterocyst differentiation, a 10.5-kb DNA element is excised from within the hupL gene by site-specific recombination between 16-bp direct repeats that flank the element. The predicted HupL polypeptide is homologous to the large subunit of [NiFe] uptake hydrogenases. hupL is expressed similarly to the nitrogen-fixation genes; hupL message was detected only during the late stages of heterocyst development. An open reading frame, named xisC, identified near one end of the hupL DNA element is presumed to encode the element's site-specific recombinase. The predicted XisC polypeptide is homologous with the Anabaena sp. strain PCC 7120 site-specific recombinase XisA. Neither XisC nor XisA shows sequence similarity to other proteins, suggesting that they represent a different class of site-specific recombinase.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Gellert M. V(D)J recombination gets a break. Trends Genet. 1992 Dec;8(12):408–412. [PubMed]
  • Oettinger MA. Activation of V(D)J recombination by RAG1 and RAG2. Trends Genet. 1992 Dec;8(12):413–416. [PubMed]
  • Tobler H, Etter A, Müller F. Chromatin diminution in nematode development. Trends Genet. 1992 Dec;8(12):427–432. [PubMed]
  • Haber JE. Mating-type gene switching in Saccharomyces cerevisiae. Trends Genet. 1992 Dec;8(12):446–452. [PubMed]
  • Haselkorn R. Developmentally regulated gene rearrangements in prokaryotes. Annu Rev Genet. 1992;26:113–130. [PubMed]
  • Kunkel B, Losick R, Stragier P. The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene. Genes Dev. 1990 Apr;4(4):525–535. [PubMed]
  • Sato T, Samori Y, Kobayashi Y. The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase. J Bacteriol. 1990 Feb;172(2):1092–1098. [PMC free article] [PubMed]
  • Golden JW, Robinson SJ, Haselkorn R. Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature. 1985 Apr 4;314(6010):419–423. [PubMed]
  • Golden JW, Whorff LL, Wiest DR. Independent regulation of nifHDK operon transcription and DNA rearrangement during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1991 Nov;173(22):7098–7105. [PMC free article] [PubMed]
  • Brusca JS, Chastain CJ, Golden JW. Expression of the Anabaena sp. strain PCC 7120 xisA gene from a heterologous promoter results in excision of the nifD element. J Bacteriol. 1990 Jul;172(7):3925–3931. [PMC free article] [PubMed]
  • Golden JW, Wiest DR. Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science. 1988 Dec 9;242(4884):1421–1423. [PubMed]
  • Lammers PJ, Golden JW, Haselkorn R. Identification and sequence of a gene required for a developmentally regulated DNA excision in Anabaena. Cell. 1986 Mar 28;44(6):905–911. [PubMed]
  • Golden JW, Carrasco CD, Mulligan ME, Schneider GJ, Haselkorn R. Deletion of a 55-kilobase-pair DNA element from the chromosome during heterocyst differentiation of Anabaena sp. strain PCC 7120. J Bacteriol. 1988 Nov;170(11):5034–5041. [PMC free article] [PubMed]
  • Mulligan ME, Haselkorn R. Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120. The nifB-fdxN-nifS-nifU operon. J Biol Chem. 1989 Nov 15;264(32):19200–19207. [PubMed]
  • Golden JW, Mulligan ME, Haselkorn R. Different recombination site specificity of two developmentally regulated genome rearrangements. Nature. 1987 Jun 11;327(6122):526–529. [PubMed]
  • Carrasco CD, Ramaswamy KS, Ramasubramanian TS, Golden JW. Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Genes Dev. 1994 Jan;8(1):74–83. [PubMed]
  • Buikema WJ, Haselkorn R. Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1991 Mar;173(6):1879–1885. [PMC free article] [PubMed]
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Kuritz T, Ernst A, Black TA, Wolk CP. High-resolution mapping of genetic loci of Anabaena PCC 7120 required for photosynthesis and nitrogen fixation. Mol Microbiol. 1993 Apr;8(1):101–110. [PubMed]
  • Przybyla AE, Robbins J, Menon N, Peck HD., Jr Structure-function relationships among the nickel-containing hydrogenases. FEMS Microbiol Rev. 1992 Feb;8(2):109–135. [PubMed]
  • Wu LF, Mandrand MA. Microbial hydrogenases: primary structure, classification, signatures and phylogeny. FEMS Microbiol Rev. 1993 Apr;10(3-4):243–269. [PubMed]
  • Leclerc M, Colbeau A, Cauvin B, Vignais PM. Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus. Mol Gen Genet. 1988 Sep;214(1):97–107. [PubMed]
  • Rousset M, Dermoun Z, Hatchikian CE, Bélaich JP. Cloning and sequencing of the locus encoding the large and small subunit genes of the periplasmic [NiFe]hydrogenase from Desulfovibrio fructosovorans. Gene. 1990 Sep 28;94(1):95–101. [PubMed]
  • Elhai J, Wolk CP. Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena. EMBO J. 1990 Oct;9(10):3379–3388. [PMC free article] [PubMed]
  • Chastain CJ, Brusca JS, Ramasubramanian TS, Wei TF, Golden JW. A sequence-specific DNA-binding factor (VF1) from Anabaena sp. strain PCC 7120 vegetative cells binds to three adjacent sites in the xisA upstream region. J Bacteriol. 1990 Sep;172(9):5044–5051. [PMC free article] [PubMed]
  • Ramasubramanian TS, Wei TF, Golden JW. Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes. J Bacteriol. 1994 Mar;176(5):1214–1223. [PMC free article] [PubMed]
  • Stark WM, Boocock MR, Sherratt DJ. Catalysis by site-specific recombinases. Trends Genet. 1992 Dec;8(12):432–439. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...