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Plant Physiol. 1981 Mar; 67(3): 565–569.
PMCID: PMC425726

Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes 1


A series of non-photoautotrophic mutants of Chlamydomonas reinhardii was isolated by replica-plating mutagenized cells which had been grown in the dark. Many of these acetate-requiring mutants are photosensitive, showing poor growth on acetate medium in the light, but normal growth in the dark. Biochemical characterization showed that the photosensitive mutants all had specific lesions in photosynthesis or photosynthetic pigment accumulation. The acetate-requiring mutants which were not photosensitive were all able to fix CO2. Among the light-sensitive mutants are 15 which show uniparental inheritance. These include six with specific lesions in photosystem II and one with an altered large subunit of ribulose-1,5-bisphosphate carboxylase. Since these two classes of uniparental mutants have been rare or not previously reported, it seems likely that photosensitivity is an important factor which limited their detection in previous mutant isolation experiments.

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Selected References

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  • Arnon DI. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. [PMC free article] [PubMed]
  • Boynton JE, Gillham NW, Chabot JF. Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function. J Cell Sci. 1972 Mar;10(2):267–305. [PubMed]
  • Chua NH, Matlin K, Bennoun P. A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii. J Cell Biol. 1975 Nov;67(2PT1):361–377. [PMC free article] [PubMed]
  • Epel BL, Levine RP. Mutant strains of Chlamydomonas reinhardi with lesions on the oxidizing side of photosystem II. Biochim Biophys Acta. 1971 Jan 12;226(1):154–160. [PubMed]
  • Goodenough UW, Levine RP. Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus. Plant Physiol. 1969 Jul;44(7):990–1000. [PMC free article] [PubMed]
  • Goodenough UW, Armstrong JJ, Levine RP. Photosynthetic Properties of ac-31, a Mutant Strain of Chlamydomonas reinhardi Devoid of Chloroplast Membrane Stacking. Plant Physiol. 1969 Jul;44(7):1001–1012. [PMC free article] [PubMed]
  • Harris EH, Boynton JE, Gillham NW. Chloroplast ribosome biogenesis in Chlamydomonas. Selection and characterization of mutants blocked in ribosome formation. J Cell Biol. 1974 Oct;63(1):160–179. [PMC free article] [PubMed]
  • Hudock MO, Togasaki RK, Lien S, Hosek M, San Pietro A. A uniparentally inherited mutation affecting photophosphorylation in Chlamydomonas reinhardi. Biochem Biophys Res Commun. 1979 Mar 15;87(1):66–71. [PubMed]
  • LEVINE RP, EBERSOLD WT. The genetics and cytology of Chlamydomonas. Annu Rev Microbiol. 1960;14:197–216. [PubMed]
  • Levine RP, Goodenough UW. The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet. 1970;4:397–408. [PubMed]
  • Levine RP, Gorman DS. Photosynthetic electron transport chain of Chlamydomonas reinhardi. 3. Light-induced absorbance changes in chloroplast fragments of the wild type and mutant strains. Plant Physiol. 1966 Oct;41(8):1293–1300. [PMC free article] [PubMed]
  • Moll B, Levine RP. Characterization of a Photosynthetic Mutant Strain of Chlamydomonas reinhardi Deficient in Phosphoribulokinase Activity. Plant Physiol. 1970 Oct;46(4):576–580. [PMC free article] [PubMed]
  • SAGER R, ZALOKAR M. Pigments and photosynthesis in a carotenoid-deficient mutant of Chlamydomonas. Nature. 1958 Jul 12;182(4628):98–100. [PubMed]
  • Schmidt GW, Matlin KS, Chua NH. A rapid procedure for selective enrichment of photosynthetic electron transport mutants. Proc Natl Acad Sci U S A. 1977 Feb;74(2):610–614. [PMC free article] [PubMed]
  • Shepherd HS, Boynton JE, Gillham NW. Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1353–1357. [PMC free article] [PubMed]
  • Wang W, Boynton JE, Gillham NW. Genetic control of chlorophyll biosynthesis in chlamydomonas: analysis of a mutant affecting synthesis of delta-aminolevulinic acid. Cell. 1975 Sep;6(1):75–84. [PubMed]
  • Wang WY, Wang WL, Boynton JE, Gillham NW. Genetic control of chlorophyll biosynthesis in Chlamydomonas. Analysis of mutants at two loci mediating the conversion of protoporphyrin-IX to magnesium protoporphyrin. J Cell Biol. 1974 Dec;63(3):806–823. [PMC free article] [PubMed]
  • Wurtz EA, Sears BB, Rabert DK, Shepherd HS, Gillham NW, Boynton JE. A specific increase in chloroplast gene mutations following growth of Chlamydomonas in 5-fluorodeoxyuridine. Mol Gen Genet. 1979 Mar 5;170(3):235–242. [PubMed]

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