• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Mar 14, 1995; 92(6): 2106–2110.
PMCID: PMC42432

Bcp1, a gene required for male fertility in Arabidopsis.

Abstract

Male fertility in flowering plants is dependent on production of viable pollen grains within the anther. Genes expressed exclusively in the anther are likely to include those that control male fertility. On the basis of their tissue specificity, such genes have been isolated, yet in none of them has this function been demonstrated. Here we report that one such gene, Bcp1, is active in both diploid tapetum and haploid microspores and is required for pollen fertility. Perturbation of this gene in either tapetum or microspores prevents production of fertile pollen in transgenic Arabidopsis plants. When tapetum expression of this gene is perturbed, mature anthers contain dead shriveled pollen. On the other hand, when microspore expression is perturbed, anthers show 1:1 segregation of viable/aborted pollen. These findings identify a class of sporophytic/gametophytic genes controlling male fertility and, hence, reproduction in flowering plants.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Regan SM, Moffatt BA. Cytochemical Analysis of Pollen Development in Wild-Type Arabidopsis and a Male-Sterile Mutant. Plant Cell. 1990 Sep;2(9):877–889. [PMC free article] [PubMed]
  • Preuss D, Lemieux B, Yen G, Davis RW. A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization. Genes Dev. 1993 Jun;7(6):974–985. [PubMed]
  • Theerakulpisut P, Xu H, Singh MB, Pettitt JM, Knox RB. Isolation and developmental expression of Bcp1, an anther-specific cDNA clone in Brassica campestris. Plant Cell. 1991 Oct;3(10):1073–1084. [PMC free article] [PubMed]
  • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
  • Xu H, Davies SP, Kwan BY, O'Brien AP, Singh M, Knox RB. Haploid and diploid expression of a Brassica campestris anther-specific gene promoter in Arabidopsis and tobacco. Mol Gen Genet. 1993 May;239(1-2):58–65. [PubMed]
  • Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. [PMC free article] [PubMed]
  • Ditta G, Stanfield S, Corbin D, Helinski DR. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. [PMC free article] [PubMed]
  • Valvekens D, Montagu MV, Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. [PMC free article] [PubMed]
  • Heslop-Harrison J, Heslop-Harrison Y. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain Technol. 1970 May;45(3):115–120. [PubMed]
  • Alexander MP. Differential staining of aborted and nonaborted pollen. Stain Technol. 1969 May;44(3):117–122. [PubMed]
  • Aarts MG, Dirkse WG, Stiekema WJ, Pereira A. Transposon tagging of a male sterility gene in Arabidopsis. Nature. 1993 Jun 24;363(6431):715–717. [PubMed]
  • Twell D, Yamaguchi J, McCormick S. Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. Development. 1990 Jul;109(3):705–713. [PubMed]
  • McCormick S. Male Gametophyte Development. Plant Cell. 1993 Oct;5(10):1265–1275. [PMC free article] [PubMed]
  • Fray RG, Grierson D. Molecular genetics of tomato fruit ripening. Trends Genet. 1993 Dec;9(12):438–443. [PubMed]
  • Vergne P, Delvallee I, Dumas C. Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization. Stain Technol. 1987 Sep;62(5):299–304. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • MedGen
    MedGen
    Related information in MedGen
  • Nucleotide
    Nucleotide
    Published Nucleotide sequences
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...