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Proc Natl Acad Sci U S A. Mar 14, 1995; 92(6): 1836–1840.
PMCID: PMC42377

Transposon-induced promoter scrambling: a mechanism for the evolution of new alleles.

Abstract

We have studied a germinal revertant of the Mutator (Mu3)-induced mutation (Adh1-3F1124) of the maize alcohol dehydrogenase 1 gene (adh1). Transposon Mu3 was inserted at the TATA box of the promoter. The excision of Mu3 caused a complex, multibreakpoint DNA rearrangement with deletion, inverted duplication, and inversions affecting 430 nucleotides in the promoter region. These changes led to an unusual pattern of adh1 gene expression: increased levels of enzyme activity in one organ, decreased levels in another, and almost unchanged levels in a third organ. The evolutionary impact of transposon-induced promoter scrambling on generation of allelic diversity is discussed. We present a fragmentation model to help explain how transposon excision could induce multiple breakpoint aberrations without involving a homologous chromosome.

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  • Gould SJ. Ontogeny and phylogeny--revisited and reunited. Bioessays. 1992 Apr;14(4):275–279. [PubMed]
  • McClintock B. The significance of responses of the genome to challenge. Science. 1984 Nov 16;226(4676):792–801. [PubMed]
  • Wessler SR. Phenotypic diversity mediated by the maize transposable elements Ac and Spm. Science. 1988 Oct 21;242(4877):399–405. [PubMed]
  • Gierl A, Saedler H. The En/Spm transposable element of Zea mays. Plant Mol Biol. 1989 Sep;13(3):261–266. [PubMed]
  • Chandler VL, Hardeman KJ. The Mu elements of Zea mays. Adv Genet. 1992;30:77–122. [PubMed]
  • Dawe RK, Lachmansingh AR, Freeling M. Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific gene expression. Plant Cell. 1993 Mar;5(3):311–319. [PMC free article] [PubMed]
  • Saedler H, Nevers P. Transposition in plants: a molecular model. EMBO J. 1985 Mar;4(3):585–590. [PMC free article] [PubMed]
  • Doseff A, Martienssen R, Sundaresan V. Somatic excision of the Mu1 transposable element of maize. Nucleic Acids Res. 1991 Feb 11;19(3):579–584. [PMC free article] [PubMed]
  • Chen CH, Oishi KK, Kloeckener-Gruissem B, Freeling M. Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics. 1987 Jul;116(3):469–477. [PMC free article] [PubMed]
  • Kloeckener-Gruissem B, Vogel JM, Freeling M. The TATA box promoter region of maize Adh1 affects its organ-specific expression. EMBO J. 1992 Jan;11(1):157–166. [PMC free article] [PubMed]
  • Gordenin DA, Lobachev KS, Degtyareva NP, Malkova AL, Perkins E, Resnick MA. Inverted DNA repeats: a source of eukaryotic genomic instability. Mol Cell Biol. 1993 Sep;13(9):5315–5322. [PMC free article] [PubMed]
  • Walker JC, Howard EA, Dennis ES, Peacock WJ. DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6624–6628. [PMC free article] [PubMed]
  • Sachs MM, Freeling M, Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. [PubMed]
  • Springer B, Werr W, Starlinger P, Bennett DC, Zokolica M, Freeling M. The Shrunken gene on chromosome 9 of Zea mays L is expressed in various plant tissues and encodes an anaerobic protein. Mol Gen Genet. 1986 Dec;205(3):461–468. [PubMed]
  • Britt AB, Walbot V. Germinal and somatic products of Mu1 excision from the Bronze-1 gene of Zea mays. Mol Gen Genet. 1991 Jun;227(2):267–276. [PubMed]
  • Plasterk RH. The origin of footprints of the Tc1 transposon of Caenorhabditis elegans. EMBO J. 1991 Jul;10(7):1919–1925. [PMC free article] [PubMed]
  • Coen ES, Carpenter R. A semi-dominant allele, niv-525, acts in trans to inhibit expression of its wild-type homologue in Antirrhinum majus. EMBO J. 1988 Apr;7(4):877–883. [PMC free article] [PubMed]
  • Raghuraman MK, Brewer BJ, Fangman WL. Activation of a yeast replication origin near a double-stranded DNA break. Genes Dev. 1994 Mar 1;8(5):554–562. [PubMed]
  • Damiani RD, Jr, Wessler SR. An upstream open reading frame represses expression of Lc, a member of the R/B family of maize transcriptional activators. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8244–8248. [PMC free article] [PubMed]
  • Stavenhagen JB, Robins DM. An ancient provirus has imposed androgen regulation on the adjacent mouse sex-limited protein gene. Cell. 1988 Oct 21;55(2):247–254. [PubMed]
  • Finnegan DJ. Eukaryotic transposable elements and genome evolution. Trends Genet. 1989 Apr;5(4):103–107. [PubMed]
  • Sandmeyer SB, Hansen LJ, Chalker DL. Integration specificity of retrotransposons and retroviruses. Annu Rev Genet. 1990;24:491–518. [PubMed]
  • Lister C, Jackson D, Martin C. Transposon-induced inversion in Antirrhinum modifies nivea gene expression to give a novel flower color pattern under the control of cycloidearadialis. Plant Cell. 1993 Nov;5(11):1541–1553. [PMC free article] [PubMed]
  • Schneuwly S, Kuroiwa A, Gehring WJ. Molecular analysis of the dominant homeotic Antennapedia phenotype. EMBO J. 1987 Jan;6(1):201–206. [PMC free article] [PubMed]

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